cell culture Methods, Protocols and Troubleshootings

Top : Forum Archives: : Indexed by Topic

Cell culture Related Discussions

What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 5)
Starting a cell line - (reply: 3)
what cell line to use for visualizing membrane localized protein - (reply: 1)
what cell line to use for visualizing membrane localized protein - (reply: 1)
Define cell passage number - (reply: 1)
THP-1 cells from Sigma - (reply: 1)
DNA "snot" in cell lysis/protein extraction - (reply: 2)
cell proliferation - (reply: 4)
DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
Is fat cell looked white after HE staining? - (reply: 10)
H.influenzae and S.pneumoniae cell culture - H.influenzae and S.pneumoniae grow on plates but not in broth (reply: 1)
What does abbreviation +ve mean? - e.g. CD45+ve cells (reply: 8)
Cells die after changing medium - Is there role of FBS? (reply: 1)
Cell line died; unknown cause... - (reply: 7)
Different cell culture plastics, problems with cell adherance - (reply: 5)
My clones disappear after subculturing! - (reply: 2)
transfecting large gene in mammalian cells - difficulties in transfecting large gene in mammalian cells (reply: 5)
Cytotoxicity assay with RAW 264.6 cells - (reply: 1)
Comparing surface protein expression of different cell lines - (reply: 1)
Decide which cell line to use - (reply: 3)
How to compare the fluorescence staining signal in cells - (reply: 6)
Cell seeding density - (reply: 11)
293T cells adhesion and transfection... - Looking for cell line for localization studies... (reply: 3)
Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
SMP30 silencing in vivo - stem cell biology (reply: 2)
growth curve? - how to establish growth curve of specific cell line.... (reply: 1)
Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
transfecting human lymphoblastoid cells - (reply: 2)
p53 status in cell lines - (reply: 3)
H9 Cell transfection - H9 cell transfection protocol (reply: 4)
Cell Adherence - (reply: 2)
Cotransfection of DNA and siRNA in Neuro2a cells - (reply: 1)
How to kill bacteria in treated cells? - (reply: 6)
where can I get hmec-1 cell line? - where can I get hmec-1 cell line? (reply: 1)
cell line to use for neural regeneration? - (reply: 1)
cell culture - (reply: 1)
Does immortalization affect non-dividing short-living cells? - (reply: 3)
transporting cell lines on board of international airflight - how? (reply: 4)
Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
ng into competents cells - (reply: 2)
Quenching oxygen in cell culture - (reply: 1)
trypsinization in what temperature will disturb less the cells? - (reply: 6)
Inhibitors in cell culture... - DMSO???? (reply: 4)
Are these cells still viable? (Trypan Blue Stain) - Using this stain for the first time against Sf9 cells (reply: 6)
% Cytotoxicity or Relative Cell Damage less than zero - What does it imply? (reply: 4)
Trypsin Assay Calculation - Please Help me!!Really in urgent (reply: 4)
3T3-L1 cells peeling/curling when differentiated - any help? - (reply: 6)
Ratio between cells and lysis buffer - (reply: 1)
cell culture - (reply: 1)
Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 3)
Nocodazole, cell cycle arrest in G2/M - (reply: 3)
What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
how to maintain iPS cells - (reply: 2)
xenografts analysis of GFP-expressing tumor cells? - (reply: 2)
Cell count in solution - (reply: 2)
Lysing cells with RIPA containing SDS - Stickiness of the lysate (reply: 5)
Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
IL4 and GMSCF for DC culture - how much IL4 and GMSCF for culturing dendritic cells (reply: 1)
Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
cell culture protocol (URGENT) - (reply: 1)
cell cryopreservation in -80 - (reply: 2)
PMA Ionomycin Cell Culture / Dilution and Math Check - (reply: 1)
Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 32)
Zymolyase treatment of yeast cell - (reply: 1)
Cell growth inhibition by lipofectamine 2000 - (reply: 1)
Immortalize mouse T cells in vitro - (reply: 1)
Trypsin concentration and preparation - How to dissolve Trypsin powder? (reply: 2)
Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
Stem Cell Niche - Murine Adult hematopoietic stem cell division Niche?? (reply: 1)
Cell death after PMA treatment - (reply: 5)
spinner flasks cell maintenance - (reply: 3)
what protease is best for primary cell culture - (reply: 1)
contamination of DH5alpha cell line - (reply: 5)
Basic immuno/histo common knowledge question - Where can I find what cells in what organ? (reply: 3)
MDCK cell experience - (reply: 2)
Help MCF7 , MDA231 cells - Treatment of cells. (reply: 1)
Expression of EGFR in MDA-MB-435 cell line - (reply: 2)
How does invitro morphology change w/cell cycle - (reply: 1)
how to distinguish monocyte and dendritic cell under microscope - (reply: 1)
protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
cell cultur medium - (reply: 3)
DNA extraction...help needed! - extracting dna from buccal cells in ethanol (i think) (reply: 5)
High-throughput screening of suspension cell lines - (reply: 2)
Cell Clumping - (reply: 1)
Single Bacterial Cell Isolation for Comet Assay - (reply: 1)
Help with clonogenic survival assay for PC3 prostate cancer cell line - HELP! (reply: 5)
FACS data analysis - understanding cell quest program and reading data from it (reply: 1)
Competent cells for transformation - (reply: 1)
Problems with cell lysis - (reply: 3)
How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
Causes for vacuoles or bubbles in cultured cells - (reply: 7)
Mycoplasma - How to detect and treat mycoplasma contamination in culture and RNA? (reply: 9)
In need of advice on HUVEC cell culturing - (reply: 17)
Post-doc position available in my lab - Leukemia stem cells (reply: 4)
what are the methods available for protein extraction from cell line? - (reply: 6)
Is it a general effect that stably transfected cell line has lower cell prolifer - (reply: 4)
Cell Counting in Hemocytometer - Teach me short cut to count in this chamber (reply: 13)
how can i seed 0.5 cell /well? - (reply: 10)
Best method to locate a receptor on breast cancer cell? - Using an AFM (reply: 2)
RPM for Pelleting down cells - (reply: 3)
how to culture cancer stem cells? - help (reply: 1)
T cell proliferation assay with macrophages - (reply: 2)
Staining suspension cells for microscopy - (reply: 1)
Problems when harvesting cells - (reply: 4)
Liver cancer primary cell culture - urgent!Thanks (reply: 1)
About red precipitate during passing the cell line - (reply: 2)
Umbilical cord blood stem cells - (reply: 1)
housekeeping gene for B cell lines - (reply: 1)
Cell lysate preparation - (reply: 2)
Who uses non-adherent cells? - like NCI-H69... are they ready to use after passing? (reply: 1)
Cell ELISA, Permeabilization cell - (reply: 1)
no pellet after centrifugation for making competent cells - (reply: 4)
Cell cycle analysis. - Cell synchronized or no-synchronized. (reply: 6)
Daudi cells transfection - (reply: 1)
express toxic protein in insect cells - (reply: 2)
Primary tracheal cell culture - (reply: 1)
Any commercially available primary cells? - Need some cardiomyocytes (reply: 1)
Need some advice on HeLa cells health hazards - (reply: 7)
two bacteria populations observed when there should be just one - how to get rid of cell doublets? (reply: 4)
Reviving THP1 cells - (reply: 6)
help with making ultra competent cell (Inoue method) - (reply: 3)
Protein Isolation of very low cell number... - (reply: 4)
How to seed less number of cells - (reply: 2)
making INV110 competent cells - (reply: 1)
"Twin-peak" @ G2/M phase? - funny result of cell cycle assay (reply: 1)
b-mercaptoethanol and stem cells - (reply: 3)
Glioma cell lines - (reply: 2)
Cell fractionation problem - Nucleaus forms a pellet that can't be suspended (reply: 5)
Storage of Cell pellet - (reply: 8)
Bradford problem in cell lysates - (reply: 1)
Intracellular proteins on live cells - (reply: 3)
Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
making a stable cell line - (reply: 3)
Medium for culturing 2 types of cell together - (reply: 2)
siRNA transfection in MLE12 cells - (reply: 3)
Transfect competent cell? - (reply: 1)
Problem in growing DG44 CHO cells - (reply: 2)
Need advice on counting low cell numbers - (reply: 1)
making stable cell line - (reply: 5)
Can I load 1N NaOH in cell culture plate? - 1N NaOH is strong alkaline, will it eat up the glass? (reply: 4)
GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
Why 10% serum for cell culture? - (reply: 11)
Cell culture basic questions - Basic questions on cell culture (reply: 7)
cell based system - (reply: 1)
flourescence in non-transfected cells! - (reply: 4)
Seeding cells on cover slips - how to get my cells to grow confluently (reply: 5)
% confluency question - when to transfect the cells? (reply: 2)
Dead cell staining on fixed cells - (reply: 1)
Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
The best way for RNA extraction from cells in RNAlater? - TRI Reagent or RNeasy or something else? (reply: 1)
Free grid image for counting cells (freeware) - simple but useful (reply: 7)
Question on cell line - (reply: 1)
Cell toxicity and color - (reply: 1)
T98G cell size - Coulter counter (reply: 1)
PCR and competent cells - (reply: 3)
TOP10 competent cell with another vector - (reply: 1)
Nuclear extracts from myeloid cells? - (reply: 9)
Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
primary Hepatocytes - experts advice - (reply: 1)
Plating cells in Serum-free medium - How to plate Mcf7 cells in a serum free medium? (reply: 7)
siRNA for retinal pigment epithelial cells - (reply: 1)
Overshot pH of Competent cell buffer (RbCl) :( - (reply: 3)
high levles of cell death in PC12 cells - (reply: 3)
Jurkat T cell imaginge - (reply: 1)
PMA - used to differentiate THP-1 cells - (reply: 6)
How much pellet do you get after using RIPA? - How long to keep cells in RIPA? (reply: 4)
Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
HOW TO MAKE A STABLE TRANSFECTED CELL LINE with COS-7? - (reply: 2)
tranwells and C6 glioblastoma cell migration - (reply: 1)
High autofluorescence in cultured cells - (reply: 8)
Cell culture contamination - (reply: 8)
Primary bone cells? where to buy - (reply: 4)
yeast cell cycle and temperature - FACS profile at 25 and 36 (reply: 1)
cells culture - (reply: 6)
Melanin extraction from melanoma cells, How? - (reply: 3)
Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
Strange black particles while growing Sf9 insect cells - (reply: 3)
PC12 cells are not "round up" - (reply: 2)
I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
cell death after catalase overexpression - (reply: 2)
FRET Imaging :Hela cells - (reply: 2)
GFP Tobacco BY-2 cell sorting - (reply: 1)
Jurkat Cells and Proliferation Assay - Click-iT EdU - (reply: 2)
Detection of GFP expressing cells - fix or not? - (reply: 3)
cell density - (reply: 2)
Cell culture unidentifiable 'contaminant' - weird things appearing in my culture (reply: 1)
3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
Cell harvest - Cell harvest (reply: 1)
How to kill yeast cells :) - chloramine or autoclaving (reply: 19)
Propagation of breast cancer cell lines in serum free media? - (reply: 1)
RNA isolation from small amount of suspension cells - (reply: 3)
Subculturing without trypsin - (reply: 2)
Culturing THP-1 cells, they grew too slow...Need help... - (reply: 11)
Poor ligation efficiency using Invitrogen TOP10 and XLI-blue cells - (reply: 5)
cell migration - (reply: 1)
Primary gastric epithelial cells - (reply: 1)
Crystal violet to determine cell numbers - (reply: 1)
Ca++ estimation in the cell - (reply: 1)
Do RAW cells (high passage number) lyse in PBS? - (reply: 2)
picking a cell line to perform cell transformation assays - (reply: 2)
red blood cells and the HA Assay - differences between species (reply: 6)
cell differentiation - (reply: 2)
How much media needed for cells in different cell culture plates - (reply: 3)
Culture of Epithelial cells from Breast Milk? - (reply: 2)
Cell separation - Cell separation (reply: 1)
HEP G2 cells - growth medium - (reply: 2)
Very high confluence after thawing cells– good or bad? - (reply: 6)
poor cell growth following FBS batch change - (reply: 5)
Cell Proliferation inhibition - arrest? (reply: 4)
CHO cells medium - (reply: 5)
Jurkat Cell Adhesion - Techniques to make Jurkat Cells to adhere to plastic surfaces (reply: 2)
Paradoxical cell count - Increase in cell count wihtout (known) stimuli (reply: 2)
Cell clumping in culture: Folic acid or i-Inositol? - (reply: 3)
Can the current technology make a human cell solely using materials from nature? - Can the current technology make a human cell solely using materials fr (reply: 2)
count cell number - (reply: 3)
Could we grwo chinese hamster cells in untreated normal petri dishes? - (reply: 1)
Could the strange G418 resistance fo my Chinese hamser cell lines be due to the - CAN I USE UNTREAED plates for selection? (reply: 2)
Is stem cell ethical? - Do you support or oppose it? (reply: 2)
stable cell screen - (reply: 2)
ES cell targeting - (reply: 2)
Are adherent THP-1 cells macrophages? - (reply: 1)
vehicle treated cells? - (reply: 2)
Microscope Settings - Microscope Settings for Trypan Blue Exclusion Assay (reply: 1)
Home made DH5alpha chemically comp. cells - (reply: 5)
siRNA transfection in endothelial cells - (reply: 3)
Growing E.coli cells - (reply: 1)
Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
IPTG induction - at what cell density (reply: 5)
how to test the efficiency of chemically competent cells - (reply: 2)
whole cell sample vs cell lysate - (reply: 2)
gene down-regulated in cancer cell line but no proliferation inhibit ability - (reply: 3)
siRNA plus drugs - weird cell morphology (reply: 3)
sudden cell death- Please HELP - (reply: 6)
Is it Okay to provide 5%CO2 to insect cells SF21? - (reply: 4)
cell adhesion problems in outer wells for transfeciton - (reply: 1)
identify cell lines - (reply: 4)
cell lysis buffer - (reply: 4)
Does Trypan Blue expire? - (reply: 3)
Tyrosinase extraction from melanoma cells - (reply: 7)
RNA isolation from small amounts of cells - (reply: 1)
bacterial cell counter - Bacterial cell counter, need to count bacterial cell by automatic inst (reply: 2)
DNA extraction from PFA fixed cells - (reply: 2)
analysis of yeast cell cycle - problem (reply: 2)
Help of establishing stable cell line - (reply: 1)
How to detach adherent cells for Annexin V staining in FACScan - (reply: 1)
Cell lysis method for running cell lysate on Western blot - (reply: 4)
culture of spleen cell - (reply: 2)
Problems with making efficient chemically competent cells - (reply: 2)
prepare the SCLC for the transfection - SCLC cell seeding for transfection (reply: 4)
N in Cell Culture - What is the proper way to determine your "n"? (reply: 2)
PFA-Fixation of cells... why do they look crappy? - PFA-fixation of epithelial cells (reply: 2)
Can cell debris move? - (reply: 4)
PBS with or without Calcium and Magnesium - When it is important to use either one in Cell culture?? (reply: 2)
MLN cell isolation....Help! - (reply: 1)
information for cell lines - OTHER than ATCC (reply: 4)
Pansorbin cells - (reply: 3)
Collenchyma or sclerenchyma cells in monocot /dicot leaves - (reply: 1)
double transfection plus siRNA - in HEK293 cells (reply: 1)
how to extract plasmids leaving bacteria cells intact - (reply: 7)
cell line culture - (reply: 2)
Clumping and contamination in cell culture - (reply: 2)
Chromosomal aberration assay for ES cells - (reply: 1)
PBS buffer for cell isolation sing dynabeads - (reply: 2)
Unexplained Cell Culture Death - Expertise needed (reply: 7)
Nuclear Extraction kit for Primary Cell? - What's a good one? (reply: 1)
transfecting siRNA into primary fetal mouse hepatocytes - (reply: 6)
Protocols for Preparation of cell lysate from tissue sample - (reply: 3)
New to Cell Culture and need some basic information - help needed (reply: 12)
Cell passage question - (reply: 4)
HEK 293T cells - problems getting proper attachment. - (reply: 9)
Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
Cell lysate at RT - (reply: 4)
plentivirus - stable cell line (reply: 1)
Monocyte derived Dendritic cells - (reply: 4)
Cell Culture Question - (reply: 3)
what is the media for HUVEC cells? - (reply: 4)
General procedure for passing (splitting) cells - (reply: 3)
Confluent 25 cm2 flask means how many cells? - quick question, need quick answer (reply: 1)
What's a good Nuclear extraction kit for primary cell? - (reply: 1)
THP-1 serum free cells - (reply: 3)
Establishing thymoctyes cell lines - (reply: 1)
Can enzyme kinetics be measured in whole cell extracts? - (reply: 3)
yeast specialists - strange behavior in yeast cells (reply: 9)
cell scraping - I DONT recommend it for subculturing (reply: 15)
media or cell problem? - (reply: 5)
what kind of contamination is this? (photos inside) - contamination in cell culture with HeLa (reply: 10)
quenching of GFP after selection of stable cells - (reply: 1)
cell lysate PAGE & blotting problem? - (reply: 1)
stable cell line, but quenched GFP? - (reply: 2)
Question about nontreated cell cultureware - (reply: 5)
PCR on Genomic DNA from cell lysate - (reply: 5)
looking for HCV positive cell lines? - (reply: 6)
dermal multipotent cells - (reply: 1)
what would happen if i dilute protease inhibitor too much in cell lysate? - (reply: 3)
cell line contamination - what to do? (reply: 7)
A problem about infecting primary T cells - (reply: 1)
contamination in MACS seperated cells - (reply: 3)
what to do with the differentiated mouse es cells - (reply: 3)
multiple plasmids in cells - (reply: 1)
What is the pI of most of proteins in cell? - (reply: 3)
Fungal contamination in cell lines! - (reply: 2)
easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
Can anyone tell me why my cells are dying? - My mouse embryonic stem cells are dying and I'm baffled as to why. (reply: 2)
plasmid transfection in hepatocytes - (reply: 1)
siRNA transfection in primary hepatocytes - (reply: 1)
primary hepatocytes as hepatocarcinogenesis model - (reply: 3)
Loading cell pellet in SDS-PAGE - (reply: 4)
Measuring cells in micrographs - (reply: 1)
What happen to my NIH3T3 cells? - (reply: 6)
CELL LYSIS - reasons behind protocols (reply: 2)
perovskite oxide nanowires in living cells - (reply: 1)
Getting Cells to adhere STRONGLY - help for high throughput screen (reply: 9)
Really, isn't there anyone who can recommend a TUNEL assay? - for adherent cells using fluorescent microscopy? (reply: 2)
competent Bascillus subtilis cells - (reply: 2)
Fixing cells in wells? - (reply: 4)
Transfection of siRNA in suspension cells - (reply: 6)
Problem with thawing cells from liquid nitrogen? - (reply: 6)
Observed tiny black dots on cell culture - (reply: 9)
Problem with mouse embryonic stem cells - contamination? - (reply: 1)
RNA extraction from plant cell cultures - (reply: 2)
Cell immortalize - (reply: 2)
Why cells should be washed before electroporation? - (reply: 2)
TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
Lysis buffer used to get cell lysates need to be sterilised? - (reply: 8)
confirming how the gene causes decreased growth/increased cell death - (reply: 4)
storing fixed cells - (reply: 4)
To keep dead cells when harvesting? - (reply: 2)
Best method of storing cultured cells for later RNA isolation - (reply: 1)
anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
how cells adhere to the surface of tissue culture flaask? - basic question (reply: 3)
how to sdudy the matter exchanges among cells? - (reply: 4)
Effect of trypsin on cells in FACS - (reply: 3)
RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
How do Phoenix cells produce virus - (reply: 1)
Transfecting P19 embryonic carcinoma cells - (reply: 2)
Restaining cells after cryopreservation - (reply: 4)
Transfecting NS0 cells - (reply: 5)
Freezing cells before lysis? - (reply: 4)
Calculating cell purity after MACS enrichment - (reply: 1)
Help with transfection of HEK cells with p53 flag - (reply: 5)
Role of Trypsin in Hep G2 culture - (reply: 1)
In vivo T cell depletion - (reply: 1)
Chemoresistance assay - How to measure the chemoresistance of cancer cells (reply: 6)
HEK cell, EGFP and PI staning - (reply: 7)
is a matrigel layer a 3d cell culture? - (reply: 6)
ligation transformation.......which chemically competent cells are the best? - (reply: 3)
how to cryopreserve HepG2 cells at -80 degrees? - (reply: 6)
irradiated 3T3 cells as feeder cells - (reply: 2)
hemin in cell cultures - (reply: 8)
Is it true that a cell line cannot be infected with virus again once it was alre - virus infection (reply: 1)
Cells not sticking to chamber slides after fixing - (reply: 8)
Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
Amount of Cell Lysate for Western Blot - (reply: 2)
Where can we purchase human microvascular endothelial cells? - (reply: 1)
stable transfected cell line in absence of G418 - (reply: 6)
stimulation of cell division/mitosis? - of non-dividing exocrine cells (reply: 4)
N2a cells differentiation - (reply: 4)
How to make Matrigel layer as thin as humanly possible? - Our technique relies on a VERY close contact of cell with coverslip (reply: 5)
Tris and cell toxicity - (reply: 4)
Maintaining stable cell line - (reply: 5)
cell differentiation and survival - (reply: 4)
Stable cell line - clone vs. population - (reply: 15)
DNA laddering in Smooth Muscle Cells - (reply: 1)
How to isolate gastric parietal cells? - Do you have protocol for isolation gastric parietal cell? (reply: 3)
Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
how to distinguish normal and cancerous osteoblast cells - (reply: 3)
EGFP stable cell line proof - (reply: 3)
How to kill proliferating cells but not arresting cells? - (reply: 3)
Does sonication of cell lysate.... - (reply: 2)
Cell contamination - Small rapidly moving black rods (reply: 18)
Stem Cell Culture Medium - Stem Cell Culture (reply: 1)
TMK 1 Cell - Where can I buy TMK 1 cells? (reply: 1)
rat bone marrow mast cell maturation - Anyone have any experience or advice? (reply: 1)
Agent to punch hole in cell membranes - (reply: 1)
Cell Viability Dyes Better than Trypan Blue - Trypan blue vs Erythrosine B vs Other? (reply: 4)
Yeast Competent Cells - (reply: 34)
Does formaldehyde crosslink completely kill cell? - (reply: 2)
cell lysate for immunoprecipitation - (reply: 2)
HeLa cells - safety concern - (reply: 2)
Transient Transfection of HeLa cells. - (reply: 1)
bc cell differentiation - T47ds (reply: 1)
Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
Where to buy gastric parietal cell lines? - (reply: 1)
reviving corneal cell line - (reply: 1)
Morphology of ES cell clones - Does this clone look like ES cells? (reply: 4)
Calculating cells/well in a 96-well plate - Calculating cells/well in a 96-well plate (reply: 8)
Can any one help to transfect these cells ? - SCN neuron transfection (reply: 2)
ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
cell counting again - (reply: 2)
Human dendritic cell line - (reply: 5)
How to send cells for immunfluorescent staining? - (reply: 8)
About trypsin without phenol red - (reply: 3)
Cells dying upon temperature shift. - (reply: 7)
Separation of inclusion bodies from cell debris - (reply: 2)
Spermatogonia stem cell culture - (reply: 4)
Rapid growth of transformed DH5 alpha cells - (reply: 4)
THP-1 cell culture problem: Cells die after thawing - (reply: 4)
number of Cos7 cells on a 10cm plate - (reply: 2)
Fixation of cells for long time storage - (reply: 1)
HL 60 cell - (reply: 1)
cell migration, cell invasion and cell proliferation assay - (reply: 5)
Concentration of miRNA in cells? - (reply: 1)
cell culture - problem with suspension CHO cell revival (reply: 2)
Some basic questions about cell fractionation and incl.bodies - (reply: 2)
Optimization of seeding cells number for MTT assay - (reply: 5)
Cell culture using laminin - (reply: 1)
MCF-7 cell grow too slow - (reply: 5)
Living cell GFP imaging - (reply: 1)
Living cell GFP imaging - (reply: 6)
lost contact inhibition=oncogenic cells? - (reply: 3)
weird S2 cells - (reply: 1)
to many dead cells for FACS - how to prepare "sheroid" cells gently for FACS (reply: 2)
neuronal cell suspension from brain - (reply: 4)
Cell isolation from tissue -- how do you tell how many viable cells you get? - (reply: 3)
Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
Interested in growth characterstics of HER2 expressing cell lines; SKOV-3,NCI-N8 - (reply: 1)
A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
cell fractionation and obtaining pure nuclei - (reply: 1)
Centrifugation speed for adherent cell line - (reply: 11)
Transfection of L929 cells with Poly (IC) - (reply: 1)
time required for cell recovery after thawing - (reply: 8)
Competent Cell Preparation - (reply: 2)
Competent Listeria Cells - (reply: 3)
Temperature for growth of skin cells - (reply: 3)
Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
how to know the plasmid or phage inside the cell? - (reply: 3)
Protein extraction from animal cells - (reply: 7)
why dendritic cell died in phagocytosis assay? - (reply: 5)
Sepration of cells from cell debris in sample - (reply: 2)
BHK Cell Transfection - (reply: 2)
Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
protein expression in NSO cells - (reply: 5)
HBSS vs PBS to deliver compounds to cells - (reply: 3)
Transferrin-alexa fluor633 can not be endocytosed by Vero cells. Who knows why? - (reply: 4)
centrifugation speed for fixed cell - (reply: 2)
Is TCA really specific in precipitating proteins from cell lysate? - (reply: 1)
competent cell contamination? - (reply: 3)
How to lyse animal cell line? - (reply: 17)
Lysis of fat cells/adipocytes - (reply: 7)
Cell Lysis - (reply: 2)
CHO-S cell - (reply: 1)
protein isolation from cell lines - membrane bound proteins (reply: 3)
cell lines - (reply: 2)
why there are bubble in cell? - (reply: 3)
On trypsin neutralizing solution - (reply: 2)
More deep ChIPping woes (Sonication Optimization) - Primary cell line.... (reply: 1)
Drug treatment enhanced cell growth? - (reply: 1)
how many cells in a mouse spleen - (reply: 5)
problem with transformation - ligation and cells seems to be fine (reply: 3)
The BEST cell counting technique? - (reply: 4)
Wanted! Protocol Immunocytochemistry with HRP-DAB reaction - Need Non-fluorescent immunostaining on cultured cells (reply: 2)
cell harvesting; use of antibiotics? - (reply: 8)
dead cell staining followed by fixation? - (reply: 4)
Best Guess:How many cells in Organotypic Slice culture - (reply: 2)
can hyaluronidase be used to detach/digest cells? - (reply: 1)
What are the negative effects of HEPES on cells - if any - (reply: 1)
Cell Suspension for MRI Study - (reply: 3)
HELP: transfection reagent for BJ cells - (reply: 1)
Search more efficient transfection reagent for BJ cells - (reply: 1)
Search transfection reagent for BJ cells - (reply: 2)
Unexplained cell death - (reply: 6)
mycoplasma effects - looking for a reference (reply: 5)
Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
Apoptosis by PI staining on epithelial cells? - (reply: 3)
what's wrong with my MTT assay on a suspension cell line - (reply: 8)
cell density for checking certain compound - (reply: 2)
Ooops! I messed up! Help! - freezing cells (reply: 5)
Dissotiation of cells from the bottom of a transwell - (reply: 2)
Co-IP in sonicated whole cell lysates - (reply: 3)
staining cells on transwell inserts - fluorescence microscopy (reply: 3)
no GFP in cells - (reply: 7)
Problem with cell harvest - cell harvest (reply: 6)
SLOW cell growth - (reply: 2)
Protein expression help needed - Mammalian cells (reply: 11)
Apyrase in cell culture - Half-life? (reply: 3)
DMSO in cell culture - is it toxic, does it change signalling? - Citations wellcommed (reply: 2)
Cells are lost after fixation - (reply: 14)
How long to wait until cell activation after trypsine use? - (reply: 6)
primary sertoli cell transfection - (reply: 2)
Force "swimming" cells to adhere - Are there any non-toxic detergents for cell culture? (reply: 4)
how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
Identification of monomers/dimers in whole cell lysate via SDS-PAGE - (reply: 3)
Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
suspension culture of lymphoblastic cells - (reply: 7)
Hela cell contamination? - (reply: 10)
THP-1 cells culture - (reply: 4)
Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
HEK 293 cell culture cytotoxicity? - (reply: 2)
brdu staining on gfp+ve cells - (reply: 1)
How to Transfect T cells - (reply: 2)
How to infect T cells? Lentiviral or retroviral? - (reply: 2)
Doesn't anyone use roller bottles to culture cells in anymore? - Let's compare notes. (reply: 2)
dendritic cells culture - (reply: 3)
help!pearl necklace like structure in CHO cell? - pearl necklace like structure in CHO cell? (reply: 1)
cell lysis and save the ECM - (reply: 1)
Pipetting stress on cells - (reply: 5)
Harvesting cells following transfection - Scrapping or trypsin (reply: 2)
losing protein expression after stablizing cells( please help) - (reply: 4)
Competent cells not growing - (reply: 5)
Equal number of cells per well (96 well plate) - (reply: 1)
preparation of bacterial cell lysate - how to do? (reply: 1)
Smooth muscle cell culture - (reply: 4)
Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
Contamination by packaging cells? - (reply: 5)
Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
best con. of trypsin with neuro cells? - (reply: 9)
Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
How to select a cell type to study my gene - (reply: 2)
Cell fractioning in order to perform binding assay - (reply: 1)
cos-7 cells transfection using calcium phosphate - (reply: 1)
competent cell didn't grow in LB - (reply: 15)
Problems in CaCl2 method ofr competent cells - (reply: 1)
BrDU staining on GFP positive cells - (reply: 1)
Electro-competent cells. How do I make them "more" competent? - Need advice on increasing the efficiency of Top 10 E. coli (reply: 10)
Contamination or not? - Cell Culture Contamination (reply: 1)
cell attachment assay - which one and how? - (reply: 6)
protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
Bradford vs 280 protein quantification - BSA standard vs cell lysates (reply: 4)
Infection of human cell lines grown in suspension? - Infection protocols (reply: 3)
cytokines anf growth factor expression in bone marrow mesenchymal stem cells - Stem cells (reply: 3)
Need advice for growing THP-1 cells - (reply: 4)
suspencion cell - electroporation with BTX830 apparatus - TROUBLE WİTH SUSPENSİON CELLS (reply: 6)
Live cell nuclei immunostaining - (reply: 2)
Contamination of cultured cells - (reply: 23)
Does a B lymphocyte contain lysosome inside? - Cell Structure (reply: 1)
stage at which transfection should be done, when cells are round or flat adhered - (reply: 4)
Cell fractionation-help! - (reply: 6)
Western blot with Adipose cells - (reply: 1)
Nystatin makes vero cells round, too high concentration? - (reply: 3)
if Annexin-v and PI staining is suitable to attached cells - (reply: 3)
How to separate cells in urine for FISH? - (reply: 1)
Dopaminergic cell lines - Need info on DAergic cell lines (reply: 2)
CaCl2 Competent Cell - (reply: 3)
western blots - survivin - detection of survivin in Panc1 cells (reply: 2)
Does trypsin digest integral plasma membrane proteins? - (reply: 2)
culturing trouble making competent cells - (reply: 1)
passage of cell culture and protein locolization - (reply: 1)
How to pick cell from soft agar and recover it? - (reply: 2)
ChIP assay using Matrigel-3D cell culture - (reply: 2)
Schock freezing - cell disruption? - (reply: 3)
quantifying my gene expression in 5 different cell lines - (reply: 1)
Culturing of MUTZ-3 cells - (reply: 1)
Counting cells using ELISA method? - Counting cells using ELISA method (reply: 1)
Adipocytes from stem cells? - (reply: 6)
trypan blue - (reply: 2)
PCR variability or bad cell source? - (reply: 1)
culture CD34+ Hematopoietic Stem Cells - (reply: 2)
help with site-directed mutagenesis - What polymerase and cells to use? (reply: 2)
Preparing cell lysate from cell culture for Western blot - (reply: 4)
Plasmid replication in mammalian cell systems - (reply: 3)
How to introduce DNA to animal cells? - (reply: 3)
Problem with growing up from cell stocks - (reply: 3)
MTT assay + suspension cells - (reply: 4)
gene targeting in ES cells - (reply: 1)
Western Blot of cells in Laemmil - (reply: 5)
neuro stem cells vs. neuroblastoma cells - (reply: 1)
AML cell lines - Where can I get them?? (reply: 1)
Clumping of normal colon cells due to transfection reagent - Counting problems (reply: 6)
prepare electrocompetent cells - (reply: 4)
why my suspension cells changed to adherent cells? - (reply: 3)
CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
3H Thymidine assay with non-adherent cells / cell harvester - cell harvester a must? alternatives? (reply: 2)
plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
human serum in cell culture - is it possible to use human serum in cell culture? (reply: 14)
Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
Just how many cells are lost when changing tubes? - (reply: 5)
collect secreted proteins in cell culture media - (reply: 2)
Cell cycle: ModFIT vs. FlowJo - diferent percentages outcomes on same histograms (reply: 1)
Where exactly are mesenchymal stem cells located in bone marrow? - (reply: 1)
problem with plating adherent cells in 24-well plates - (reply: 11)
Jurkat/J77 cell line specifics - (reply: 2)
Raji Cell Histone Immunocytochemistry and Confocal Help - (reply: 1)
DNA isolation from small amount of cells - (reply: 1)
Cell Seeding and Drug treatment - (reply: 12)
unable to trasnfect Hela cells - (reply: 6)
Embryonic stem cell culture - (reply: 4)
DOES DMSO CHANGE THE CELL? - (reply: 2)
cell biology video clips - (reply: 2)
androgen solution for cell treatment - (reply: 7)
cell seeding confusion - what does it mean (reply: 1)
Basic help in cell counting...... - (reply: 6)
Using Adenovirus to transfect cells - (reply: 5)
PPARs expression in cardiac cells - (reply: 4)
Cell assay for ROS measurment - (reply: 1)
How do I culture adherent TZM-bl cells?! - (reply: 5)
Red Cells in Clear Ficoll Layer Following Density Centrifugation - Monocyte Isolation (reply: 2)
Breaking yeast cells - (reply: 2)
preparation of electroporation competent cells from chemically competent cells - (reply: 3)
cotransfection of CHO cells - (reply: 1)
cell culture - passage number (reply: 7)
can plasma (instead of serum) be used on cell culture? - (reply: 2)
do the competen cells chang the U to T - (reply: 4)
RAW cell line transfection - (reply: 2)
increased BrDU incorporation but similiar cell cycle profile by PI or DAPI - (reply: 2)
When should I suspect there is a Mycoplasma contamination? - (reply: 10)
help! cell culture contamination too! - (reply: 5)
E.coli TOP10 comp cells T1 Phage resistant? - (reply: 1)
recommendations for a viral titer cell line - (reply: 3)
what time is better to seed cells in well or dishes before transfection? - (reply: 7)
Subculturing primary human fibroblasts - Seeding density? (reply: 1)
reusable cell culture flasks, pipettes etc. - (reply: 10)
Experiment with KATO III cells - (reply: 1)
please give me some suggestions on preparating Yeast cells with PI stainning fo - buffer ,PI solution (reply: 4)
SiRNA in MIN6 cells, nucleofection - (reply: 1)
Single cell luciferase bioluminescence detection. Is it possible? - (reply: 1)
Cell passage number for non-cancerous cells - (reply: 2)
Sodium pyruvate in ES cell culture media - (reply: 2)
Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
Fixing non adherent cells - (reply: 3)
stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
A good list of literatures for primary cell isolation - (reply: 3)
Homework question - what happens to cells when they're put in distilled wate - (reply: 3)
How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
looking for protocol using DAPI for cell cycle analysis - (reply: 1)
Whats the cell size - (reply: 1)
Synchronization of NIH 3T3 cells - (reply: 2)
B95-8 EBV producing cell line - need EBV producing cell line. (reply: 2)
difference between staining vital cell and dead - (reply: 9)
why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
Question making competent bacterial cells - (reply: 2)
Enlarging Cells - (reply: 3)
Cell Growth Following Confluency - (reply: 2)
Stable cell line losing expression faster than usual - (reply: 6)
Transfecting primary cells in suspension - (reply: 4)
PCR from cell culture - to detect the presence of adenovirus (reply: 2)
primary colonic epithelial cell culture - (reply: 1)
Passaging stable transfected cells after thawing - (reply: 4)
trypsin concentrations for detaching cells - (reply: 2)
Cell culture - (reply: 2)
storing unstained cells overnight for FACS next day - (reply: 3)
Is that possible to establish a stable cell line which is transfected by an empt - (reply: 2)
Cell adheison assy on round surface - (reply: 1)
Could competent cell be stored in liquid nitrogen? - (reply: 7)
Effectively washing adherent cells? - how do I optimize washing? (reply: 3)
what happens if cells turn liquid? - (reply: 4)
Formaldehyde cross link after washing cells - (reply: 2)
Cell Fixation method - % of PFA ? (reply: 4)
cell synchronization - how to make cell stay at G0 phase (reply: 1)
culture cell - (reply: 1)
lysing cells on matrigel - (reply: 1)
Storing Induced E.Coli Cells - (reply: 1)
Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
choosing competent cells - (reply: 5)
extractin RNA from cells grown on Matrigel - (reply: 1)
FACS sorting and culturing cells afterwards - (reply: 1)
siRNA stable cell lines - (reply: 5)
Secrets for declumping cell pellets - (reply: 5)
Best compotent cells - (reply: 5)
Mycoplasma - (reply: 2)
cell density for infection with baculovirus - what is the optimal cell dentisty at the point of infection (reply: 1)
mouse U6 promotor in human ES cells - (reply: 1)
Creating Cell suspension - (reply: 1)
Cell Surface Biotinylation - Advantage/Disadvantage (reply: 7)
pyridine, chloroform and metahnol as a solvent for chemopreventive agents - Is there anyone who has used these solvent for cell culture experiment (reply: 6)
changed attchment of RMA/S cells after transfection, why? - (reply: 2)
cell culture contamination - after transfection (reply: 15)
T antigen status of human cells for transfection - (reply: 1)
How to prepare my own competent cells? - as above (reply: 5)
removing rbc cells for cell purification - (reply: 5)
minimum amount of cells used for western blot - (reply: 5)
cell lysis and DNA - sticky (reply: 3)
Is it possible to transform competent cells with linear DNA? - (reply: 5)
293T cells - (reply: 3)
natural killer cells - (reply: 2)
Frustrated with FACS (ONLY DEAD CELLS) - (reply: 8)
Problems obtaining stable transfected 293 cells - (reply: 1)
few cells in the cell culture - (reply: 3)
Help with different cell fixatives - Neuronal Fixation (reply: 7)
counting viable cells directly from wells - (reply: 7)
Stable transfection of H295R cells - (reply: 3)
how do i do flow cytometry for adherent cells? - (reply: 5)
tranfection of 3T3-L1 cells post differentiation - (reply: 2)
Problem with Monoclonal antibody production - why cells die after adding HAT?????? (reply: 6)
stainig of cells for FACS - (reply: 2)
Trypsin/EDTA - how it works? (reply: 1)
pBABE-puro in 293T cells (not PhoenixA) - (reply: 5)
Basic CHO cell culture - (reply: 3)
stable transfections using SCC 9 cells - (reply: 5)
Which vector would you choose for transient expression in CHO cells - (reply: 2)
Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
Alternative Sera for Cell Culture - (reply: 6)
293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
E.coli Compotent Cell Storage and Plasmid Isolation - (reply: 3)
How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
Cell culture sterility Checks (DMEM) - (reply: 3)
PMA induced apoptosis in HEK cells - (reply: 4)
Use of urea for cell lysis - (reply: 1)
Why different mammalian cell lysate preparation methods? - (reply: 6)
how to maintain cell in serum free medium - (reply: 6)
cell lysis to investigate phosphorylated protein - (reply: 1)
growing cells on coverslip - (reply: 6)
centrifugation speed for mouse neural stem cells - centrifugation speed for mouse neural stem cells (reply: 3)
GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
cy5 cell tracker dye - (reply: 2)
Which cell line for Cell cycle analysis - (reply: 1)
transformation competent cells problem - fastbac vectors (reply: 2)
RT-QPCR of cells treated with 5-aza - which housekeeping gene to use, different cell lines (reply: 1)
Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
cell starvation - which medium is good? (reply: 4)
transformation of competent cells - (reply: 2)
Electroporation with a pool of vectors with different inserts - why only one kind of vector maintained in the cells? (reply: 2)
Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
freezing cells in log phase - (reply: 2)
adipose stains? - specific stain to positively ID adipose cells (reply: 2)
chromatin yield (ChIP) - chromatin yield from primary cells (reply: 3)
Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
Cell washing - Can I use Tween/Triton etc? (reply: 7)
isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
Poly-lysine treatment and cell removing - (reply: 4)
SURE E. coli strain - How make competents cells? (reply: 4)
Monocytic cell line - (reply: 1)
APC isolation from the Liver - How you can isolate the APC cells from the mice's liver (reply: 2)
thawed cells dying - (reply: 6)
Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
stable transfection for suspension cell lines - (reply: 1)
differentiated PC12 cells detatching - (reply: 3)
automatic cell counting for Microscope images - (reply: 2)
Thp-1 cell transduction - (reply: 4)