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  1. cell lysate colorless? - (reply: 1)
  2. Does anyone else gas their cells like this?! - (reply: 5)
  3. Transient transfection trouble- massive cell death - (reply: 1)
  4. animal cell lysis, on plates - (reply: 2)
  5. selection of transfected cells - (reply: 1)
  6. Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3)
  7. How to count cells on plate - (reply: 3)
  8. Transformation of non-competent E. coli? - Any shortcut for preapring competent cells? (reply: 2)
  9. boosting expression in stable cell line? - (reply: 5)
  10. too few cells used for electroporation/ transfection - (reply: 4)
  11. any way to inactivate trypsin without using FBS? - (and still let cells survive ^__^) (reply: 3)
  12. Quantification of VEGF peptide levels in human breast cancer cells. HELP! - (reply: 6)
  13. making Stocks of HL 60 cells - making Stocks of HL 60 cells (reply: 1)
  14. Mycoplasma contamination. - (reply: 6)
  15. cell and tissue culture - healthy test (reply: 1)
  16. Need human cells for a science fair project - (reply: 4)
  17. THP-1 cell culture - (reply: 3)
  18. Mammalian expression problem - HEK cells expression (reply: 3)
  19. Contamination and its fungus - persistant fungal contamination in cell cultures (reply: 1)
  20. Removing all cell surface proteins/receptors - (reply: 3)
  21. The action of alcohol on bacteria cells - Washing bacteria cell (resuspending) pellets with methanol? (reply: 2)
  22. PBS for releasing anchored cells, what kind? - (reply: 2)
  23. Which part of the kidney are COS-7 cells from? - (reply: 1)
  24. Chemical transformation in 96 well MTP - How to make competent cells in MTPs (reply: 2)
  25. Do COS-7, CHO-K1 and LLC-PK1 cells express dopamine receptors? - I need an answer soon... (reply: 2)
  26. Is it hard to make knock-out cells/mice? - (reply: 4)
  27. trypsin - wat is the source of trypsin we use in cell culture (reply: 1)
  28. Competent Cells - (reply: 1)
  29. PC12 cells do not attach on collagen - (reply: 4)
  30. cell fractionation and low protein concentration in cytosol - (reply: 1)
  31. IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
  32. Adding some PBS to cells - (reply: 3)
  33. Quantifying Cell Lysis or the Presence of DNA - (reply: 3)
  34. What exactly are CD4+3- inducer cells? - simply a specific form of "T-helper-cells"? (reply: 2)
  35. In vitro toxicology test by animal cell line - Looking for methods (reply: 1)
  36. inoculating liquid media from frozen cell stock ok? - (reply: 6)
  37. Cell treating - Do you... (reply: 4)
  38. cell lysis - (reply: 3)
  39. How to fixate cells onto glass microscope slide - (reply: 2)
  40. Is there any destructive effect on nucleic acid when harvest the cell by scrapin - (reply: 1)
  41. Transfected copies per cell - (reply: 1)
  42. RAW 264 cells - (reply: 1)
  43. How to concentrate retrovirus produced by Phoenix-Ampho cell - (reply: 4)
  44. Have I killed my cells? Doh! - (reply: 3)
  45. Looking for specific cancer cell line - Need a parent line with metastatic and non-metastatic lines (reply: 2)
  46. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
  47. mesenchymal stem cell differentiation into osteoblasts (alizarin) - (reply: 2)
  48. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
  49. siRNA into RAW264.7 cells? - any ideas (reply: 4)
  50. Heat-shock treatment problems with adherent cells - (reply: 1)
  51. No Retrovirus using the Phoenix - Ampho cells - (reply: 1)
  52. IEC-6, IL-6 and LPS - Cannot get my IEC-6 cells to secrete Il-6 (reply: 2)
  53. Creating stable cell lines - (reply: 4)
  54. Cholesterol Assay - Commerically available cholesterol assay kit for cell extracts (reply: 1)
  55. Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
  56. Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
  57. cell culture question - passage number - (reply: 5)
  58. A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
  59. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
  60. 293t Cell Attachment Issues - (reply: 7)
  61. giant cells? - (reply: 6)
  62. Direct Cell Lysis in Laemmli - (reply: 1)
  63. Cell Morphology - (reply: 1)
  64. When to use PMSF in a cell lysis buffer? - (reply: 9)
  65. staining for receptors in cell line - (reply: 1)
  66. Counting B cells in hemacytometer - (reply: 2)
  67. Coating cell dishes with peptides - (reply: 2)
  68. Does it look like cell clumps/artefact/contamination? - (reply: 3)
  69. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
  70. what cell line to use for visualizing membrane localized protein - (reply: 1)
  71. Define cell passage number - (reply: 2)
  72. THP-1 cells from Sigma - (reply: 1)
  73. DNA "snot" in cell lysis/protein extraction - (reply: 6)
  74. DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
  75. H.influenzae and S.pneumoniae cell culture - H.influenzae and S.pneumoniae grow on plates but not in broth (reply: 1)
  76. Cells die after changing medium - Is there role of FBS? (reply: 1)
  77. Different cell culture plastics, problems with cell adherance - (reply: 6)
  78. transfecting large gene in mammalian cells - difficulties in transfecting large gene in mammalian cells (reply: 5)
  79. Comparing surface protein expression of different cell lines - (reply: 1)
  80. How to compare the fluorescence staining signal in cells - (reply: 6)
  81. what cells to use for expression of pGEX3? - (reply: 1)
  82. What's "polarized T cell"? - (reply: 1)
  83. SMP30 silencing in vivo - stem cell biology (reply: 2)
  84. Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
  85. p53 status in cell lines - (reply: 3)
  86. Cell Adherence - (reply: 2)
  87. How to kill bacteria in treated cells? - (reply: 6)
  88. cell line to use for neural regeneration? - (reply: 1)
  89. Does immortalization affect non-dividing short-living cells? - (reply: 3)
  90. Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
  91. Quenching oxygen in cell culture - (reply: 1)
  92. what is trypsin assay by BAEE - (reply: 2)
  93. Are these cells still viable? (Trypan Blue Stain) - Using this stain for the first time against Sf9 cells (reply: 6)
  94. Trypsin Assay Calculation - Please Help me!!Really in urgent (reply: 4)
  95. Ratio between cells and lysis buffer - (reply: 1)
  96. Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 4)
  97. What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
  98. NK Cell Isolation - negative selection kits - (reply: 1)
  99. Cell count in solution - (reply: 2)
  100. Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
  101. Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
  102. cell cryopreservation in -80 - (reply: 2)
  103. Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
  104. Cell growth inhibition by lipofectamine 2000 - (reply: 1)
  105. T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
  106. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
  107. Cell death after PMA treatment - (reply: 5)
  108. what protease is best for primary cell culture - (reply: 1)
  109. Basic immuno/histo common knowledge question - Where can I find what cells in what organ? (reply: 4)
  110. Help MCF7 , MDA231 cells - Treatment of cells. (reply: 1)
  111. How does invitro morphology change w/cell cycle - (reply: 1)
  112. protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
  113. DNA extraction...help needed! - extracting dna from buccal cells in ethanol (i think) (reply: 5)
  114. Cell Clumping - (reply: 1)
  115. Help with clonogenic survival assay for PC3 prostate cancer cell line - HELP! (reply: 5)
  116. Competent cells for transformation - (reply: 1)
  117. How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
  118. Mycoplasma - How to detect and treat mycoplasma contamination in culture and RNA? (reply: 9)
  119. Post-doc position available in my lab - Leukemia stem cells (reply: 4)
  120. Is it a general effect that stably transfected cell line has lower cell prolifer - (reply: 4)
  121. how can i seed 0.5 cell /well? - (reply: 10)
  122. RPM for Pelleting down cells - (reply: 3)
  123. how to culture cancer stem cells? - help (reply: 1)
  124. Staining suspension cells for microscopy - (reply: 1)
  125. Liver cancer primary cell culture - urgent!Thanks (reply: 1)
  126. Umbilical cord blood stem cells - (reply: 1)
  127. Cell lysate preparation - (reply: 2)
  128. Cell ELISA, Permeabilization cell - (reply: 1)
  129. Cell cycle analysis. - Cell synchronized or no-synchronized. (reply: 6)
  130. express toxic protein in insect cells - (reply: 2)
  131. Any commercially available primary cells? - Need some cardiomyocytes (reply: 1)
  132. two bacteria populations observed when there should be just one - how to get rid of cell doublets? (reply: 4)
  133. help with making ultra competent cell (Inoue method) - (reply: 3)
  134. How to seed less number of cells - (reply: 2)
  135. "Twin-peak" @ G2/M phase? - funny result of cell cycle assay (reply: 1)
  136. Glioma cell lines - (reply: 2)
  137. Storage of Cell pellet - (reply: 8)
  138. Intracellular proteins on live cells - (reply: 3)
  139. making a stable cell line - (reply: 3)
  140. siRNA transfection in MLE12 cells - (reply: 3)
  141. Problem in growing DG44 CHO cells - (reply: 2)
  142. making stable cell line - (reply: 5)
  143. GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
  144. Cell culture basic questions - Basic questions on cell culture (reply: 7)
  145. flourescence in non-transfected cells! - (reply: 4)
  146. % confluency question - when to transfect the cells? (reply: 2)
  147. Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
  148. Free grid image for counting cells (freeware) - simple but useful (reply: 7)
  149. Cell toxicity and color - (reply: 1)
  150. PCR and competent cells - (reply: 3)
  151. Nuclear extracts from myeloid cells? - (reply: 9)
  152. primary Hepatocytes - experts advice - (reply: 1)
  153. siRNA for retinal pigment epithelial cells - (reply: 1)
  154. high levles of cell death in PC12 cells - (reply: 3)
  155. PMA - used to differentiate THP-1 cells - (reply: 6)
  156. Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
  157. tranwells and C6 glioblastoma cell migration - (reply: 1)
  158. Cell culture contamination - (reply: 9)
  159. yeast cell cycle and temperature - FACS profile at 25 and 36 (reply: 1)
  160. Melanin extraction from melanoma cells, How? - (reply: 3)
  161. Strange black particles while growing Sf9 insect cells - (reply: 3)
  162. I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
  163. FRET Imaging :Hela cells - (reply: 2)
  164. Jurkat Cells and Proliferation Assay - Click-iT EdU - (reply: 2)
  165. cell density - (reply: 2)
  166. 3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
  167. How to kill yeast cells :) - chloramine or autoclaving (reply: 19)
  168. RNA isolation from small amount of suspension cells - (reply: 3)
  169. Culturing THP-1 cells, they grew too slow...Need help... - (reply: 11)
  170. cell migration - (reply: 1)
  171. Crystal violet to determine cell numbers - (reply: 1)
  172. Do RAW cells (high passage number) lyse in PBS? - (reply: 2)
  173. red blood cells and the HA Assay - differences between species (reply: 6)
  174. How much media needed for cells in different cell culture plates - (reply: 3)
  175. Cell separation - Cell separation (reply: 1)
  176. Very high confluence after thawing cells– good or bad? - (reply: 8)
  177. Cell Proliferation inhibition - arrest? (reply: 4)
  178. Jurkat Cell Adhesion - Techniques to make Jurkat Cells to adhere to plastic surfaces (reply: 2)
  179. Cell clumping in culture: Folic acid or i-Inositol? - (reply: 3)
  180. count cell number - (reply: 3)
  181. Could the strange G418 resistance fo my Chinese hamser cell lines be due to the - CAN I USE UNTREAED plates for selection? (reply: 2)
  182. stable cell screen - (reply: 2)
  183. Are adherent THP-1 cells macrophages? - (reply: 1)
  184. Microscope Settings - Microscope Settings for Trypan Blue Exclusion Assay (reply: 1)
  185. siRNA transfection in endothelial cells - (reply: 3)
  186. Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
  187. IPTG induction - at what cell density (reply: 5)
  188. whole cell sample vs cell lysate - (reply: 2)
  189. siRNA plus drugs - weird cell morphology (reply: 3)
  190. Is it Okay to provide 5%CO2 to insect cells SF21? - (reply: 4)
  191. identify cell lines - (reply: 4)
  192. Does Trypan Blue expire? - (reply: 3)
  193. RNA isolation from small amounts of cells - (reply: 1)
  194. DNA extraction from PFA fixed cells - (reply: 2)
  195. Help of establishing stable cell line - (reply: 1)
  196. Cell lysis method for running cell lysate on Western blot - (reply: 4)
  197. Problems with making efficient chemically competent cells - (reply: 2)
  198. N in Cell Culture - What is the proper way to determine your "n"? (reply: 2)
  199. Can cell debris move? - (reply: 4)
  200. MLN cell isolation....Help! - (reply: 1)
  201. Pansorbin cells - (reply: 3)
  202. double transfection plus siRNA - in HEK293 cells (reply: 1)
  203. cell line culture - (reply: 2)
  204. Chromosomal aberration assay for ES cells - (reply: 1)
  205. Unexplained Cell Culture Death - Expertise needed (reply: 7)
  206. transfecting siRNA into primary fetal mouse hepatocytes - (reply: 6)
  207. New to Cell Culture and need some basic information - help needed (reply: 12)
  208. HEK 293T cells - problems getting proper attachment. - (reply: 9)
  209. Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
  210. plentivirus - stable cell line (reply: 1)
  211. Cell Culture Question - (reply: 3)
  212. General procedure for passing (splitting) cells - (reply: 3)
  213. What's a good Nuclear extraction kit for primary cell? - (reply: 1)
  214. Establishing thymoctyes cell lines - (reply: 1)
  215. yeast specialists - strange behavior in yeast cells (reply: 9)
  216. media or cell problem? - (reply: 5)
  217. quenching of GFP after selection of stable cells - (reply: 1)
  218. stable cell line, but quenched GFP? - (reply: 5)
  219. PCR on Genomic DNA from cell lysate - (reply: 5)
  220. dermal multipotent cells - (reply: 1)
  221. cell line contamination - what to do? (reply: 7)
  222. contamination in MACS seperated cells - (reply: 3)
  223. multiple plasmids in cells - (reply: 1)
  224. Fungal contamination in cell lines! - (reply: 2)
  225. Can anyone tell me why my cells are dying? - My mouse embryonic stem cells are dying and I'm baffled as to why. (reply: 2)
  226. siRNA transfection in primary hepatocytes - (reply: 1)
  227. Loading cell pellet in SDS-PAGE - (reply: 4)
  228. What happen to my NIH3T3 cells? - (reply: 6)
  229. perovskite oxide nanowires in living cells - (reply: 1)
  230. Really, isn't there anyone who can recommend a TUNEL assay? - for adherent cells using fluorescent microscopy? (reply: 2)
  231. Fixing cells in wells? - (reply: 4)
  232. Problem with thawing cells from liquid nitrogen? - (reply: 6)
  233. Problem with mouse embryonic stem cells - contamination? - (reply: 1)
  234. Cell immortalize - (reply: 2)
  235. TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
  236. confirming how the gene causes decreased growth/increased cell death - (reply: 4)
  237. To keep dead cells when harvesting? - (reply: 2)
  238. anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
  239. how to sdudy the matter exchanges among cells? - (reply: 4)
  240. RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
  241. Transfecting P19 embryonic carcinoma cells - (reply: 2)
  242. Transfecting NS0 cells - (reply: 5)
  243. Calculating cell purity after MACS enrichment - (reply: 1)
  244. Role of Trypsin in Hep G2 culture - (reply: 1)
  245. Chemoresistance assay - How to measure the chemoresistance of cancer cells (reply: 6)
  246. is a matrigel layer a 3d cell culture? - (reply: 6)
  247. how to cryopreserve HepG2 cells at -80 degrees? - (reply: 6)
  248. hemin in cell cultures - (reply: 8)
  249. Cells not sticking to chamber slides after fixing - (reply: 8)
  250. Amount of Cell Lysate for Western Blot - (reply: 2)
  251. stable transfected cell line in absence of G418 - (reply: 6)
  252. N2a cells differentiation - (reply: 4)
  253. Tris and cell toxicity - (reply: 4)
  254. cell differentiation and survival - (reply: 4)
  255. DNA laddering in Smooth Muscle Cells - (reply: 1)
  256. Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
  257. EGFP stable cell line proof - (reply: 3)
  258. Does sonication of cell lysate.... - (reply: 2)
  259. Stem Cell Culture Medium - Stem Cell Culture (reply: 1)
  260. rat bone marrow mast cell maturation - Anyone have any experience or advice? (reply: 1)
  261. Cell Viability Dyes Better than Trypan Blue - Trypan blue vs Erythrosine B vs Other? (reply: 4)
  262. Does formaldehyde crosslink completely kill cell? - (reply: 2)
  263. HeLa cells - safety concern - (reply: 2)
  264. bc cell differentiation - T47ds (reply: 1)
  265. Where to buy gastric parietal cell lines? - (reply: 1)
  266. Morphology of ES cell clones - Does this clone look like ES cells? (reply: 4)
  267. Can any one help to transfect these cells ? - SCN neuron transfection (reply: 2)
  268. cell counting again - (reply: 2)
  269. How to send cells for immunfluorescent staining? - (reply: 8)
  270. Cells dying upon temperature shift. - (reply: 7)
  271. Spermatogonia stem cell culture - (reply: 4)
  272. THP-1 cell culture problem: Cells die after thawing - (reply: 4)
  273. Fixation of cells for long time storage - (reply: 1)
  274. cell migration, cell invasion and cell proliferation assay - (reply: 5)
  275. cell culture - problem with suspension CHO cell revival (reply: 2)
  276. Optimization of seeding cells number for MTT assay - (reply: 5)
  277. MCF-7 cell grow too slow - (reply: 5)
  278. Living cell GFP imaging - (reply: 6)
  279. weird S2 cells - (reply: 1)
  280. neuronal cell suspension from brain - (reply: 4)
  281. Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
  282. A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
  283. Centrifugation speed for adherent cell line - (reply: 11)
  284. time required for cell recovery after thawing - (reply: 8)
  285. Competent Listeria Cells - (reply: 3)
  286. Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
  287. Protein extraction from animal cells - (reply: 7)
  288. Sepration of cells from cell debris in sample - (reply: 2)
  289. Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
  290. HBSS vs PBS to deliver compounds to cells - (reply: 3)
  291. centrifugation speed for fixed cell - (reply: 2)
  292. competent cell contamination? - (reply: 3)
  293. Lysis of fat cells/adipocytes - (reply: 7)
  294. CHO-S cell - (reply: 1)
  295. cell lines - (reply: 2)
  296. On trypsin neutralizing solution - (reply: 2)
  297. Drug treatment enhanced cell growth? - (reply: 1)
  298. problem with transformation - ligation and cells seems to be fine (reply: 3)
  299. Wanted! Protocol Immunocytochemistry with HRP-DAB reaction - Need Non-fluorescent immunostaining on cultured cells (reply: 2)
  300. dead cell staining followed by fixation? - (reply: 4)
  301. can hyaluronidase be used to detach/digest cells? - (reply: 1)
  302. Cell Suspension for MRI Study - (reply: 3)
  303. Search more efficient transfection reagent for BJ cells - (reply: 1)
  304. Unexplained cell death - (reply: 6)
  305. Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
  306. what's wrong with my MTT assay on a suspension cell line - (reply: 8)
  307. Ooops! I messed up! Help! - freezing cells (reply: 5)
  308. Co-IP in sonicated whole cell lysates - (reply: 3)
  309. no GFP in cells - (reply: 7)
  310. SLOW cell growth - (reply: 2)
  311. Apyrase in cell culture - Half-life? (reply: 3)
  312. Cells are lost after fixation - (reply: 14)
  313. primary sertoli cell transfection - (reply: 2)
  314. how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
  315. Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
  316. Hela cell contamination? - (reply: 10)
  317. Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
  318. brdu staining on gfp+ve cells - (reply: 1)
  319. How to infect T cells? Lentiviral or retroviral? - (reply: 2)
  320. dendritic cells culture - (reply: 3)
  321. cell lysis and save the ECM - (reply: 1)
  322. Harvesting cells following transfection - Scrapping or trypsin (reply: 2)
  323. Competent cells not growing - (reply: 5)
  324. preparation of bacterial cell lysate - how to do? (reply: 1)
  325. Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
  326. Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
  327. Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
  328. Cell fractioning in order to perform binding assay - (reply: 1)
  329. competent cell didn't grow in LB - (reply: 15)
  330. BrDU staining on GFP positive cells - (reply: 1)
  331. Contamination or not? - Cell Culture Contamination (reply: 1)
  332. protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
  333. Infection of human cell lines grown in suspension? - Infection protocols (reply: 3)
  334. Need advice for growing THP-1 cells - (reply: 4)
  335. Live cell nuclei immunostaining - (reply: 2)
  336. Does a B lymphocyte contain lysosome inside? - Cell Structure (reply: 1)
  337. Cell fractionation-help! - (reply: 6)
  338. Nystatin makes vero cells round, too high concentration? - (reply: 3)
  339. How to separate cells in urine for FISH? - (reply: 1)
  340. CaCl2 Competent Cell - (reply: 3)
  341. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
  342. passage of cell culture and protein locolization - (reply: 1)
  343. ChIP assay using Matrigel-3D cell culture - (reply: 2)
  344. quantifying my gene expression in 5 different cell lines - (reply: 1)
  345. Counting cells using ELISA method? - Counting cells using ELISA method (reply: 1)
  346. trypan blue - (reply: 2)
  347. culture CD34+ Hematopoietic Stem Cells - (reply: 2)
  348. Preparing cell lysate from cell culture for Western blot - (reply: 4)
  349. How to introduce DNA to animal cells? - (reply: 3)
  350. MTT assay + suspension cells - (reply: 4)
  351. Western Blot of cells in Laemmil - (reply: 5)
  352. AML cell lines - Where can I get them?? (reply: 1)
  353. prepare electrocompetent cells - (reply: 4)
  354. CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
  355. plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
  356. Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
  357. Just how many cells are lost when changing tubes? - (reply: 5)
  358. Cell cycle: ModFIT vs. FlowJo - diferent percentages outcomes on same histograms (reply: 1)
  359. problem with plating adherent cells in 24-well plates - (reply: 11)
  360. Raji Cell Histone Immunocytochemistry and Confocal Help - (reply: 1)
  361. Cell Seeding and Drug treatment - (reply: 12)
  362. Embryonic stem cell culture - (reply: 4)
  363. cell biology video clips - (reply: 2)
  364. cell seeding confusion - what does it mean (reply: 1)
  365. Using Adenovirus to transfect cells - (reply: 5)
  366. Cell assay for ROS measurment - (reply: 1)
  367. Red Cells in Clear Ficoll Layer Following Density Centrifugation - Monocyte Isolation (reply: 2)
  368. preparation of electroporation competent cells from chemically competent cells - (reply: 3)
  369. cell culture - passage number (reply: 7)
  370. do the competen cells chang the U to T - (reply: 4)
  371. increased BrDU incorporation but similiar cell cycle profile by PI or DAPI - (reply: 2)
  372. help! cell culture contamination too! - (reply: 5)
  373. recommendations for a viral titer cell line - (reply: 3)
  374. Subculturing primary human fibroblasts - Seeding density? (reply: 1)
  375. Experiment with KATO III cells - (reply: 1)
  376. SiRNA in MIN6 cells, nucleofection - (reply: 1)
  377. Cell passage number for non-cancerous cells - (reply: 2)
  378. Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
  379. stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
  380. Homework question - what happens to cells when they're put in distilled wate - (reply: 3)
  381. looking for protocol using DAPI for cell cycle analysis - (reply: 1)
  382. Synchronization of NIH 3T3 cells - (reply: 2)
  383. difference between staining vital cell and dead - (reply: 9)
  384. Question making competent bacterial cells - (reply: 2)
  385. Cell Growth Following Confluency - (reply: 2)
  386. Transfecting primary cells in suspension - (reply: 4)
  387. primary colonic epithelial cell culture - (reply: 1)
  388. trypsin concentrations for detaching cells - (reply: 2)
  389. storing unstained cells overnight for FACS next day - (reply: 3)
  390. Cell adheison assy on round surface - (reply: 1)
  391. Effectively washing adherent cells? - how do I optimize washing? (reply: 3)
  392. Formaldehyde cross link after washing cells - (reply: 2)
  393. cell synchronization - how to make cell stay at G0 phase (reply: 1)
  394. lysing cells on matrigel - (reply: 1)
  395. Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
  396. extractin RNA from cells grown on Matrigel - (reply: 1)
  397. siRNA stable cell lines - (reply: 6)
  398. Best compotent cells - (reply: 5)
  399. cell density for infection with baculovirus - what is the optimal cell dentisty at the point of infection (reply: 1)
  400. Creating Cell suspension - (reply: 1)
  401. pyridine, chloroform and metahnol as a solvent for chemopreventive agents - Is there anyone who has used these solvent for cell culture experiment (reply: 6)
  402. cell culture contamination - after transfection (reply: 15)
  403. How to prepare my own competent cells? - as above (reply: 5)
  404. minimum amount of cells used for western blot - (reply: 5)
  405. Is it possible to transform competent cells with linear DNA? - (reply: 5)
  406. natural killer cells - (reply: 2)
  407. Problems obtaining stable transfected 293 cells - (reply: 1)
  408. Help with different cell fixatives - Neuronal Fixation (reply: 7)
  409. Stable transfection of H295R cells - (reply: 3)
  410. tranfection of 3T3-L1 cells post differentiation - (reply: 2)
  411. stainig of cells for FACS - (reply: 2)
  412. pBABE-puro in 293T cells (not PhoenixA) - (reply: 5)
  413. stable transfections using SCC 9 cells - (reply: 5)
  414. Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
  415. any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
  416. 293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
  417. How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
  418. PMA induced apoptosis in HEK cells - (reply: 4)
  419. Why different mammalian cell lysate preparation methods? - (reply: 6)
  420. cell lysis to investigate phosphorylated protein - (reply: 1)
  421. centrifugation speed for mouse neural stem cells - centrifugation speed for mouse neural stem cells (reply: 3)
  422. cy5 cell tracker dye - (reply: 2)
  423. transformation competent cells problem - fastbac vectors (reply: 2)
  424. Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
  425. transformation of competent cells - (reply: 2)
  426. Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
  427. adipose stains? - specific stain to positively ID adipose cells (reply: 2)
  428. Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
  429. isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
  430. SURE E. coli strain - How make competents cells? (reply: 4)
  431. APC isolation from the Liver - How you can isolate the APC cells from the mice's liver (reply: 2)
  432. Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
  433. differentiated PC12 cells detatching - (reply: 3)
  434. Thp-1 cell transduction - (reply: 4)
  435. HIV-2 and MTT assay, cells lines to use - (reply: 2)
  436. tissue preparation for human/mouse islet cells - (reply: 1)
  437. Protein Extraction from cell media for western blot - (reply: 3)
  438. cell adhesion to plastic - (reply: 3)
  439. Can I use a cell free extract on Mass Spec? - (reply: 8)
  440. Chemically competent cells? - (reply: 3)
  441. cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
  442. bath sonicator for cell lysis - (reply: 2)
  443. serum starvation - induce cilia by serum starvation in Rpe-1 cells (reply: 7)
  444. Keep growing cells after an MTS/PMS assay - (reply: 1)
  445. RNA in HeLa cell - amount of RNA in a HeLa cell (reply: 2)
  446. Cell fractionation: nuclei do not pellet - (reply: 2)
  447. Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
  448. lentiviral transduction of suspension cell - (reply: 1)
  449. cell counting dilemma - (reply: 1)
  450. lentiviral transduction of suspension cell - (reply: 1)
  451. Immunostaining on bacterial cells? - (reply: 1)
  452. any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
  453. FACS sorting affects cell function? - FACS sorting affects cell function (reply: 2)
  454. cell counting calculation.... - (reply: 3)
  455. Phoenix 293T packaging cells - (reply: 3)
  456. suparnatant, cell free extract, crude extract - (reply: 4)
  457. competent cells transform 12 kb plasmid,urgent - (reply: 8)
  458. Whole cell extract on SDS/PAGE - (reply: 9)
  459. Making competent cells from current commercial stocks - (reply: 3)
  460. Calculation cell in 96 well plate - (reply: 1)
  461. G418 and resistant cells - can't kill the cells (reply: 8)
  462. MIN6 cells - (reply: 9)
  463. cell signaling & choice of the cell - (reply: 3)
  464. hard to transfect cell lines - (reply: 1)
  465. How can I separate nuclei from cytoplasm in trophoblastic cells - (reply: 1)
  466. salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
  467. Primary culture - Transparent cell? (reply: 2)
  468. transfection of chick cell - (reply: 3)
  469. non-adhernt cell washing - (reply: 1)
  470. Immunology on fixed bacteria - What are the basics of fixing cells for immunological analysis? (reply: 1)
  471. transfection of PC12 cell line - (reply: 1)
  472. Assaying apoptosis on non-detaching cell culture plates - which plates? (reply: 1)
  473. does anyone know how to prepare cell extract for Metabolic and Enzymatic Assays - (reply: 6)
  474. What is the right shape for NRK cells - (reply: 1)
  475. Why fix cells? - Advantages-Disadvantages (reply: 14)
  476. Please recommend a colon cancer cell line - what is the widely used colon cancer cell line? (reply: 3)
  477. cd3- and cd8+ cells - flow cytometry cell population question (reply: 4)
  478. confocal microscope, my cells die! - URGENT. (reply: 8)
  479. transfection with PA-1 cells - (reply: 1)
  480. How to separate the antigen presenting cell from spleen of rat - (reply: 1)
  481. Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
  482. Immunofluorescence staining of spheroid cells - (reply: 3)
  483. How small cells can Flow cytometry detect? - Flow cytometry to detect a small cell (reply: 2)
  484. tg1 and hb2151 cells - (reply: 2)
  485. How to avoid central cell seeding in 6-well plates - (reply: 19)
  486. Same cell line but different growth rate - (reply: 4)
  487. Fixing Cells for Flowcytometry - Glutaraldehyde, Formalin, Microwave (reply: 11)
  488. estimating number of cells to seed - (reply: 2)
  489. cell dead after changing the medium to PBS buffer - (reply: 3)
  490. PC12 cell culture. Do I have to coat plates first? - (reply: 2)
  491. Can scintillation fluid be used to harvest adherent cells? - (reply: 1)
  492. Difference between cell invasion and cell migration - (reply: 4)
  493. too many dead cells why? - (reply: 3)
  494. Do cells transfected with lentivirus express specific unique surface markers - (reply: 2)
  495. Help: Cell Lysing - (reply: 5)
  496. Avoiding cell clumps - (reply: 7)
  497. can cells just stop having the gene? - (reply: 4)
  498. how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
  499. question about dendritic cells and antigen-specific T cells - (reply: 1)
  500. CD3 and CD28 T cell stimulation in TUBES - Technical question (reply: 4)
  501. cell wall protein extraction of gram-positive - (reply: 1)
  502. How to do the lysis of cells without white stuff? - (reply: 1)
  503. cell culture - a beginner - (reply: 3)
  504. any video or animation of experiments about cell and molecular biology - (reply: 1)
  505. how to calculate cell number? - (reply: 1)
  506. Flow Cytometry 2 Parameter Statistics, please help - Can you find standard deviation of % Positive of Total cells based on (reply: 1)
  507. cell concentration calculation - (reply: 2)
  508. isolating of mouse spleen Dendritic cells by nycodenz - (reply: 4)
  509. Suspension cell culture. - (reply: 5)
  510. PC12-Neurite out growth - less no of cells with neurite (reply: 4)
  511. p53 independent cell death - (reply: 2)
  512. Dendritic cell line media - Dendritic cell line media (reply: 1)
  513. Assay for cell purity determination - (reply: 2)
  514. fungal contamination - fungal in cell culture (reply: 4)
  515. Double-Thymidine block of Hela cells - (reply: 3)
  516. endothelial cell isolation from mouse - using magnetic dynabeads from DYNAL (Invitrogen) (reply: 6)
  517. OD600 and cell density correlation? - (reply: 1)
  518. T cell activation via CD3 and/or Cd28 need to solve a problem - T cell activation cd3 (reply: 5)
  519. Plant cell culture- How do prevent differentiation of callus cells - (reply: 2)
  520. Confocal microscopy with primary cell cultures - (reply: 8)
  521. stable cell line characterization and validation - (reply: 1)
  522. Trouble concentrating cell extract -please help! - (reply: 9)
  523. cell synchronization - (reply: 1)
  524. Mycoplasma detection - (reply: 3)
  525. Can transcriptional regulation research be done in vivo? - No cell line is availbale (reply: 7)
  526. making competent SURE cells - (reply: 2)
  527. cell line rna extraction - (reply: 1)
  528. Cell lysis - (reply: 5)
  529. Suspension cells - (reply: 4)
  530. cell growth and colorimetric assay - (reply: 1)
  531. How to maintain pH of designed culture media? - Autoclaving, metabolism of cells cause changes in pH (reply: 2)
  532. Another statistics data analysis Q - How to evaluate colony formation from sorted CHO cell (reply: 1)
  533. NK92 cell line - (reply: 2)
  534. EDTA to weaken cell attachement - (reply: 1)
  535. ccdB competent cells - (reply: 2)
  536. No more apoptosis, no less proliferation but less cells... - (reply: 4)
  537. cell line - (reply: 2)
  538. why my BSA contained medium cannot nourish my cells - (reply: 13)
  539. Trying to lyse cells to get PCR template - Need something simpler than a miniprep (reply: 10)
  540. cell culture - FBS (reply: 1)
  541. how to count the rounded cells in the well? - (reply: 4)
  542. Cell culture contamination, antibiotic recommendation, any recommd on Normocin - (reply: 7)
  543. Problem transfecting large plasmid into HEK293 cells - (reply: 8)
  544. T cell proliferation/activation markers - CD69, CD38? (reply: 1)
  545. cell loss upon centrifugation - (reply: 5)
  546. Crystal violet and State's cell - (reply: 4)
  547. How to tell my cells are contaminated? - (reply: 3)
  548. Help needed! - HUVEC - C cells - RGD targeting using Human Umbilical Endothelial Cell Line - C (reply: 2)
  549. gene expression study - between treated and untreated cells (reply: 2)
  550. Inconsistency of the cells~~ - (reply: 4)
  551. Quantifying HeLa cells in a 96 well plate - (reply: 2)
  552. Epithelium cell purification - (reply: 2)
  553. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
  554. Stable cell line stops growing :( - (reply: 6)
  555. siRNA protocol in floating cells - (reply: 1)
  556. Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
  557. Basics of Cell Culture - (reply: 2)
  558. if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
  559. Explant culture - All method of cell isolation coming from animal tissue (reply: 3)
  560. E.Coli. XA90 cell - transformation in XA90 cell (reply: 3)
  561. cells - (reply: 2)
  562. protocol for purificaiton of human mononucleus cells - (reply: 2)
  563. How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
  564. cell membrane - (reply: 6)
  565. Endothelial cells purification - (reply: 2)
  566. How to make mES cells attch to differentiate? - (reply: 1)
  567. linear or supercoiled plasmid for creating stable cell line - (reply: 1)
  568. why some microorganisms form filament instead of single cell? - (reply: 1)
  569. can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (reply: 3)
  570. concentration of trypan blue... - please help me. (reply: 8)
  571. how to determine the length of each phase in cell cycle - (reply: 1)
  572. help biochemical pathway ! - lactate effect on mammalian cells (reply: 3)
  573. Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
  574. bEND cells proliferation assay? - (reply: 1)
  575. MCF-7 cells clumped and too small - (reply: 7)
  576. Cells showing interesting morphology with treatment - How do I know what's happening (reply: 3)
  577. where to buy cell lines? - (reply: 3)
  578. effect of TE buffer on cells with cell lysis solution - (reply: 2)
  579. single channel and whole cell - (reply: 2)
  580. Funny looking PC12-TrkB cells - (reply: 1)
  581. How to break down the plant cell wall? - (reply: 1)
  582. Cell frozen - (reply: 1)
  583. transfecting adherent cells - before adherence? - (reply: 5)
  584. Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
  585. typical cell lysate protein concentration - (reply: 6)
  586. Stable cell trasfection questions... - Integration of plasmid... (reply: 5)
  587. Establishing cell lines from brain tumor tissue samples - (reply: 2)
  588. mammary stem cells - (reply: 1)
  589. ICC of cell on coverslips - losing cells at permeabilization - (reply: 2)
  590. A protocol needed to purify cancer cells from tumor tissue - need help! (reply: 4)
  591. How to disassociate cells for FACS? - (reply: 7)
  592. Myc tag and endogenous myc - Is there endogenous myc expressed in some fibreblast cells? (reply: 3)
  593. How to keep cells healthy after transfection - Any tips? (reply: 5)
  594. Culture of normal human mammary epithelial cells - (reply: 2)
  595. cell culture - (reply: 2)
  596. Starving cells for stimulation - (reply: 2)
  597. how can I detach infected vero cells from 96 well plate without lose sample? - (reply: 2)
  598. transient transfection of a stable cell line? - (reply: 4)
  599. I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
  600. WB with cell culture medium - (reply: 2)
  601. plasmid purification from mammalian cells? - (reply: 4)
  602. help- G0 synchrony of MCF10 A breast cells and MCF-7 cell lines - (reply: 1)
  603. Get rid of cell debris after transformation? - (reply: 1)
  604. monocytic cell line differentiation - (reply: 2)
  605. Obtaining cells from human tissue samples - (reply: 2)
  606. Changes on cells. - (reply: 5)
  607. 3T3-L1 cell differentiation - (reply: 2)
  608. stable cell line questions( cell type matter) - (reply: 2)
  609. Cell Culture with Human Plasma - (reply: 2)
  610. cell saparation from lymph nodes and spleen - how can I improve my yield? (reply: 1)
  611. G418 conc for HEK293 cells - (reply: 11)
  612. Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
  613. Transient transfection on a stable cell line - (reply: 2)
  614. Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
  615. Infection efficiency of non-adherent cells - (reply: 3)
  616. Are there TGFB receptors in 293T cells? - ..and if you don't know, how would you find out? (reply: 2)
  617. Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
  618. PC 12 cell line - (reply: 4)
  619. detachment of cells - (reply: 5)
  620. how to identify cancer stem cell from sp cell?what is its characteristic? - cancer stem cell (reply: 2)
  621. prevent cell attachment - (reply: 2)
  622. Cell Banking - cell splittting and banking (reply: 1)
  623. can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
  624. Help: Tubulin expression is not the same between my cell clones? - (reply: 1)
  625. Overlay cell culture medium with oil against evaporation - which oil? (reply: 2)
  626. Why EDTA in trypsin? - (reply: 28)
  627. Tumour cells grow slower than normal cells, why? - cell proliferation rate in normal vs tumour cell (reply: 2)
  628. paraformaldehyde solution - for cell fixation (reply: 19)
  629. How to seed cells evenly in small well plates - (reply: 27)
  630. remedy against cell contamination - (reply: 4)
  631. stain similar to neutral red - stain unfixed cells directly (reply: 4)
  632. How to lift attached CD14 cells? - (reply: 3)
  633. How can i decontaminated my cell culture ? - (reply: 4)
  634. Cell fixation problem - (reply: 3)
  635. Hela cells and stable transfection help please! - (reply: 6)
  636. Is cell culture considered in vivo? - (reply: 4)
  637. Transfecting KG 1 cell - (reply: 3)
  638. cell stain, nonfluroescent - (reply: 4)
  639. Need to Slow down Cell Culture growth - (reply: 2)
  640. What size needles I should use for cell extraction protein - (reply: 1)
  641. Cell adhesion assay - (reply: 2)
  642. Tetracycline in cell culture media - (reply: 5)
  643. Treatment of cancer cell - (reply: 2)
  644. why centrifuge before doing a cell count? - (reply: 1)
  645. amount of proteins isolated from cells - (reply: 2)
  646. clumping cells - s. aureus wildtyp cells (reply: 3)
  647. Primary Hippocampal cell culture-DEAD! - (reply: 3)
  648. How long can cells survive at 4 degree - (reply: 2)
  649. Medium for adherent cells and serum/protein free - (reply: 2)
  650. Mouse ES cell won't attach - (reply: 1)
  651. monitor cell growth - (reply: 1)
  652. RNA extraction from formaldehide fixed cells - (reply: 2)
  653. vector for expression of tag on cell surface - (reply: 7)
  654. Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
  655. why competent cells take up only 1 type of plasmids? - (reply: 7)
  656. cell lysis buffer recipe - simple question (reply: 6)
  657. Help with HeLa cells culture... - (reply: 10)
  658. FACSing of MCF-7 cells - (reply: 3)
  659. Transfecting primary cells (imr90) - PROBLEM - (reply: 4)
  660. Why my cells dont grow - hippocampal cell (reply: 5)
  661. PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
  662. treat cells with progesterone and estrogen - (reply: 1)
  663. difference between treating cells with ATP disodium salt and ATP magnesium salt - (reply: 2)
  664. cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
  665. growth rate of SK-BR-3 cells - (reply: 3)
  666. neural stem cell markers? - (reply: 2)
  667. G418-selected cells do not divide - (reply: 8)
  668. unwanted cell clumping after splitting MCF7 cells - (reply: 5)
  669. hematopoietic stem cell trans-differentiation - (reply: 4)
  670. T cell culture - (reply: 2)
  671. differentiating live from dead bacterial cells - (reply: 2)
  672. Long-time storage of tissues/cells with fluorescent tracers - (reply: 4)
  673. Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
  674. Why is non-viral transfection efficiency of endothelial cells low? - (reply: 1)
  675. Hepg2 cells resistant to trypsin digestion - (reply: 7)
  676. Immunofluorescence of frozen cell culture inserts - (reply: 6)
  677. T cell proliferation assay - (reply: 1)
  678. Stem Cell growth on soft agar - (reply: 3)
  679. Cell transfection - TransIT vs Fugen - (reply: 3)
  680. Debris or particles in medium - A549 cells and weird particles (reply: 4)
  681. far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
  682. Cell surface antigens - identification (reply: 8)
  683. How long can the whole cell extract for ChIP stay in -80C? - (reply: 1)
  684. Overexpression of Viral Protein in mammalian cell line - (reply: 3)
  685. Chemotaxis Assay for Primary Mouse Peritoneal Cells - (reply: 7)
  686. Getting rid of the dead cells during selection of transfected colonies? - (reply: 2)
  687. optimizing selection of transfected cells - (reply: 2)
  688. MTS assay of MCF7 and MDA-MB-453 cells - (reply: 1)
  689. primary cell culture death - (reply: 8)
  690. ELISA IL12 p70 from mouse dendritic cells culture SN - (reply: 2)
  691. new lab, cells die after passaging - general fish cell culture methods (reply: 5)
  692. Fibroblast cell line - (reply: 2)
  693. FBS or FCS for NIH3T3 cells - How to grow these cells optimal (reply: 7)
  694. Cell density to platte after trypsinization - (reply: 3)
  695. 3T3 cells and lysis buffer recepie - western blot (reply: 1)
  696. Starvation of cells for higher uptake? - (reply: 2)
  697. mouse spleen cell culture - (reply: 2)
  698. a question about splitting MCF-7 cells - (reply: 5)
  699. Trypan blue cell death assay on cardiac myocytes - (reply: 3)
  700. What is an acceptable CO2% range for growing cells in DMEM? - (reply: 8)
  701. mycoplasma effects on cells - will mycoplasma kill the cells? (reply: 2)
  702. method for staining of invisible cell pellets? - (reply: 1)
  703. rna isolation from bone marrow cells - (reply: 1)
  704. Monocyte-derived DC generation: CD14 separation or adherent cells better? - (reply: 1)
  705. GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
  706. transplantable rats or mice cell line in nude mice - (reply: 1)
  707. How to tell cell are contaminated by fungi - (reply: 4)
  708. Need protocol: CFSE Staining/ primary cells - (reply: 2)
  709. why do you prepare heat-stable fractions of cells? - (reply: 1)
  710. Puromycin and HEK293 cells - (reply: 3)
  711. neuronal markers for the mature level for spinal cord cell - (reply: 4)
  712. tissue n cell culture - (reply: 3)
  713. do cells benefit from being of different age? - (reply: 2)
  714. cell culture contamination - (reply: 2)
  715. Can I call my cell a established cell line? - (reply: 1)
  716. how long can cell lines be carried? - (reply: 5)
  717. Could we freeze the 0.25 trypsin EDTA and thaw it, use it? - (reply: 8)
  718. transfection of HeLa cells - (reply: 4)
  719. cell stain and the fixation? - (reply: 2)
  720. Cell Differentiation - Time periods and environment for proper cell differentiation (reply: 1)
  721. Cell Lysis - (reply: 2)
  722. Does anybody have MIN6 cells? - MIN6 Cells (reply: 1)
  723. SiRNA and GFP transfection MCF7 cells - (reply: 4)
  724. questions about MCF-7 cells - (reply: 10)
  725. detachment of my cells?/ - (reply: 7)
  726. depolymerizing microtubules in 293 cells - (reply: 1)
  727. cell proliferation assays - (reply: 2)
  728. Retroviral transduction of cells - troubleshooting help please! - (reply: 8)
  729. After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
  730. should dead cell detach? - (reply: 2)
  731. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  732. Stem Leydig cells - (reply: 1)
  733. adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity (reply: 3)
  734. problems with Clonetech 10G cells - (reply: 1)
  735. THP-1 cell line - Passage number affect phagocytosis (reply: 12)
  736. MTT test for the suspension culture - Raji cell and K562 (reply: 4)
  737. maximum plasmids can be co-transfected into Hela cells - (reply: 4)
  738. T cell proliferation assay - (reply: 3)
  739. Culture of adherent SH-Sy5y Neuroblastoma Cell Line - (reply: 5)
  740. CCR5 cytometry on dendritic cells, probles with isotype control - (reply: 2)
  741. lymphocytes and hepatocytes are the same!? - (reply: 4)
  742. transfection of MCF7 cells - (reply: 2)
  743. Has anyone transfected ST15A cells? - Transfection of ST15A cells? (reply: 3)
  744. cell harvest by scraping versus trypsinization - (reply: 21)
  745. ethanol/IPA kill cell - (reply: 1)
  746. Cell culture Contamination - Again! (reply: 13)
  747. PBS and Cells - How long can cells sit in PBS? (reply: 6)
  748. Freeze/Thaw procedure in cell lysis - How is this done??? (reply: 12)
  749. cell death during transfection - (reply: 3)
  750. Best way to detect shRNA produced in cells? - (reply: 2)
  751. flow cytometry - labelling surface receptor on monolayer cells (reply: 5)
  752. PMA activation of cells - (reply: 5)
  753. losing green light form pIRES2-EGFP transfected cells - (reply: 13)
  754. Help, Neuroscience cell lines - (reply: 1)
  755. How to trace siRNA in cells - (reply: 4)
  756. stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
  757. Impact of dead cells in a culture? - When cells die, how toxic can they become to viable cells? (reply: 4)
  758. Removing adherent cells for Flow cytometry without trypsin - how to do it (reply: 3)
  759. Glioblastoma cell lines and transfection - stable transfection (reply: 5)
  760. Problem establishing a stable PC12 cell line - (reply: 6)
  761. How to make 3T3 mouse fibroblast cell bank? - (reply: 1)
  762. how to know that ur cells are confluent? - (reply: 2)
  763. How to make a stable cell line? Help! - (reply: 3)
  764. time when cells go into apoptosis - (reply: 2)
  765. doxorubicin treated cell cutlures? - (reply: 1)
  766. Cell line passage number? - (reply: 3)
  767. basic techniques for cell treatment - (reply: 6)
  768. Trypsin-Free Detachment of cells using EDTA - (reply: 10)
  769. The mechanism of the cell "fix" reagent? - (reply: 1)
  770. adding antibiotics to cho cell media - (reply: 2)
  771. Can the monocytic cell line MD (ATTC no. CRL-9850) be differentiated into dendri - (reply: 1)
  772. ELISA with fixed bacterial cells - (reply: 1)
  773. cells not adhering to flask after thawing - (reply: 14)
  774. obtaining cell clone? - (reply: 3)
  775. establishment of stable cell line - (reply: 2)
  776. cell cetrifugation_how fast can you go? - (reply: 6)
  777. What's the maximum amount of DMSO you can use in cell culture? - (reply: 4)
  778. how to avoid MEF when mES cells are transfected by lentivirus? - (reply: 4)
  779. TB1 Competenet Cell - (reply: 2)
  780. 293 cell lines - (reply: 4)
  781. Growing PC12 cells on glass - (reply: 3)
  782. how to set up a stable cell line? - why false positive always appears? (reply: 2)
  783. RNA extraction from cells grown in matrigel - (reply: 1)
  784. Is there a quicker way of making stable cell line? - (reply: 7)
  785. How to deplete calcium from cell medium? - (reply: 1)
  786. Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
  787. cell separation by centrifugation - (reply: 1)
  788. cell line nonresponsiveness - is it a time to change my cell line???? (reply: 2)
  789. Cell cycle progression and arrest - (reply: 1)
  790. Cheap and quick cell viability assay - (reply: 11)
  791. siRNA transfection of MIN6 cells - (reply: 2)
  792. Is it possible to harvest E.coli cells for purification of DNA from LB agar and - (reply: 5)
  793. Cells survived after G418 selection are transfected cells? - (reply: 2)
  794. Saos-2 cells transfection - (reply: 1)
  795. how to make neutrophil cell lysates for Western blot? - (reply: 3)
  796. getting rid of shaky hands during cell culture/pipetting - (reply: 9)
  797. Mouse endothelial cell line - (reply: 1)
  798. trypsin peptide extraction storage - (reply: 3)
  799. infected cell line? - fungal contamination? (reply: 5)
  800. Growing cell lines from frozen tissue samples - Is this even possible??? (reply: 8)
  801. Cell Growth - Help! (reply: 7)
  802. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
  803. Trypsin:EDTA cell detachment process - (reply: 6)
  804. Trypan Blue exclusion - (reply: 2)
  805. Cell lysing for western blot (Help!) - (reply: 2)
  806. retroviral transfection of pheonix cells - (reply: 3)
  807. how to build a stable transfection in a suspension cell - (reply: 1)
  808. How to culture stable cell lines? - (reply: 3)
  809. effect of enzymes on cell membrane - (reply: 4)
  810. confocal assay for FITC-labled peptide in Hela cell - (reply: 1)
  811. Primary culture hepatocytes of mouse - (reply: 2)
  812. Techniques for counting apoptotic cells - (reply: 6)
  813. Persistent cell culture contamination by fungi and bacteria - Cell culture, cell biology (reply: 6)
  814. Adult stem cell differentiation - (reply: 2)
  815. Cell Lysates for western - (reply: 4)
  816. transfecting DNA into Jurkat cell - (reply: 1)
  817. single cells from human tissue? - need protocols (reply: 1)
  818. Tryphan Blue staining for cell viability - (reply: 1)
  819. Anyone has experience to grow sespension 293 cells? - (reply: 1)
  820. Cornea Epithelial Cell culture - (reply: 1)
  821. what kind of adherent cell lines can be analyzed by FCM through Annexin V/PI sta - (reply: 2)
  822. cell cycle data normalization - (reply: 1)
  823. Why are lysosomes considered the cell's "cleanup crew"? - i need help (reply: 1)
  824. Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
  825. Can I extract nuclear proteins in transfected cells? - (reply: 2)
  826. Ordering CHO-K1 cells - (reply: 1)
  827. why my IFN gamma treated cells still high in TER? - (reply: 1)
  828. co-transfection in NRK cells - (reply: 1)
  829. 293T cell line, how to prevent cell clusters? - (reply: 1)
  830. Cell cycle analysis - Include floating cells or not?? (reply: 7)
  831. Death of Cells After Defrosting - MDBK cells not surviving (reply: 2)
  832. Extensive cell death after transfection in a 24-well plate - (reply: 3)
  833. Hela cells clumping in center of well of 96well plate. - (reply: 1)
  834. mouse B cells - (reply: 1)
  835. reasons for apoptosis in primary cell culture? - (reply: 2)
  836. Problems with cell lysate - (reply: 4)
  837. competent cells - making compentent cells (reply: 2)
  838. Establishment of human embryonic stem cell - (reply: 3)
  839. Help: got the stable cell line without my target gene - (reply: 3)
  840. Sticky cells - (reply: 3)
  841. ask for 721.221 cell line - (reply: 3)
  842. Spectrophotomery of cell 550 vs. 600 - spectrophotometry for cells (reply: 2)
  843. extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
  844. looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
  845. endothelial cells transfection - (reply: 2)
  846. How many passages cells lines can survive before senescence? - (reply: 8)
  847. RNA extraction from small number of cells - (reply: 3)
  848. NMDA tox and Rat Cerebellar Granular Cells ! - NMDA tox assay for Rat CGC's ???? (reply: 3)
  849. Host cells for pBELOBAC11 - (reply: 4)
  850. URGENT-Selecting for transformed bacteria cells - (reply: 1)
  851. mammalian cells invaded by intracellular microbes - how can I decontaminate? (reply: 3)
  852. RAW 264.7 cell line transfection - (reply: 1)
  853. change adherent cells into suspenion cells - (reply: 1)
  854. detection of GFP fluorescence in whole cell bacteria - (reply: 1)
  855. How to fix and stain lymphoblastoid cell onto glass slides - (reply: 5)
  856. no growth on plate agar for competent cell - (reply: 6)
  857. starvation medium recipe for Hela cells - (reply: 2)
  858. Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (reply: 1)
  859. transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
  860. siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
  861. problem transfection Hela cells - (reply: 8)
  862. How to study the viability of cells - (reply: 10)
  863. cell lysis? - (reply: 2)
  864. cell death after subculture - (reply: 3)
  865. can you introduce mutation directly into the cells? - other than just transfecting it with a vector... (reply: 1)
  866. Mystery - Cell Proliferation Assay - (reply: 6)
  867. Help with Cell attachment problem - (reply: 3)
  868. How many cells to lyse for Western? - Decreasing signal in my Western Blot (reply: 2)
  869. Human Neural Stem Cells wanted - (reply: 1)
  870. plating cells for transfection - (reply: 2)
  871. cytotoxicity and cell proliferation assay - preference of dyes for cell viability (reply: 2)
  872. protocol for electrocompetent cells preparation - (reply: 5)
  873. Transient transfection on a stable cell line - (reply: 6)
  874. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
  875. need help for culture protacal of new stem cell - (reply: 5)
  876. Activating human T cells - (reply: 8)
  877. Storage of whole cell lysates - How? (reply: 2)
  878. Why use PBS before trypsinizing cells - (reply: 4)
  879. Question about doing RNAi in primary cells - (reply: 3)
  880. A Procedure for Freezing and Thawing Bacterial Cells - (reply: 2)
  881. Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
  882. Cell Tracing Software - Searching for a quicker way!!! (reply: 2)
  883. cells going away - (reply: 3)
  884. pituitary cell lines sex - (reply: 1)
  885. Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (reply: 4)
  886. How to tell if competent cells or SOC are contaminated? - (reply: 14)
  887. Transcient transfection of HaCaT cells - which is the most effective method? - (reply: 2)
  888. Dissociation of HaCaT cells without using trypsin - will PBS/EDTA work? - (reply: 4)
  889. Where to get Granta 536 Cells - (reply: 4)
  890. Moving Dots in cell culture - Contamination? - (reply: 7)
  891. Drosophila cell culture - (reply: 3)
  892. Culture problems of CRL-2331 cells (ATCC) - (reply: 3)
  893. electroporation of PC12 cells - (reply: 1)
  894. RNA prep (Trizol) with small cell numbers - How low can you go? (reply: 13)
  895. Slow growing cells - (reply: 6)
  896. About cell diameter - Help,please browse (reply: 1)
  897. Protoreaction of Protoplasm - New approaches to Cell Biology and Physiology (reply: 1)
  898. Problems with Ba/F3 cell culture - (reply: 1)
  899. Washing with Trypsin EDTA - (reply: 2)
  900. CHO Cell revival - (reply: 2)
  901. How to isolate and culture murine epidermal langerhans cells - (reply: 2)
  902. Neural Marker For Cell Typing and Maturation - Neural Marker For Cell Typing and Maturation (reply: 4)
  903. Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy (reply: 3)
  904. Stem cell transfection - (reply: 2)
  905. cell detachment - help (reply: 3)
  906. Able C and Able K competent cels - Has anyone use these cells before (reply: 2)
  907. bioinformatics in cell signalling - use of bioinformatics (reply: 1)
  908. Marker for plasma cell staining - (reply: 1)
  909. AGS Cell - Medium for AGS Cells (reply: 2)
  910. how to isolate T cell and DC in the same sample? - (reply: 2)
  911. Minipreps from Genehogs cell line - (reply: 1)
  912. Sick primary cells - (reply: 1)
  913. Does anyone know some specific marker of mouse hepatocytes? - (reply: 2)
  914. How to culture rat dorsal root ganglia cell? - (reply: 1)
  915. Protocal for DAPI/ Hoechst staining adhering cells - (reply: 3)
  916. how 2 quantify bacteria cells? - (reply: 4)
  917. Shipping cells in suspension culture - (reply: 6)
  918. hela cells - (reply: 12)
  919. cre recombinase - in cell lines (reply: 2)
  920. siRNA transfection reagent for primary cultured cells - (reply: 2)
  921. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
  922. Cells are stuck - (reply: 4)
  923. Serum for cell culture - difference on regular vs dialyzed vs heat-inactivated FBS (reply: 3)
  924. Red Blood Cells exploding ! - (reply: 2)
  925. How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
  926. Baculovirus Expression - Sonicate infected cells? (reply: 2)
  927. Synchronize hela cells with double thymidine block - mechanism of double thymidine block (reply: 1)
  928. house-keeping primers for Cos7 cells - (reply: 4)
  929. RAW264.7 Cells .. anyone familiar with this cell line? - need help please (reply: 2)
  930. adapting cells to CO2-Independent Medium - (reply: 1)
  931. DNA methylation in Raji cell line - (reply: 1)
  932. How long does take the cell cycle in HepG2 cell line? - (reply: 1)
  933. T cell activation. - (reply: 1)
  934. Alizarin red/Trypan blue stain - (reply: 3)
  935. coat culture dish for HEK-293T cells? - (reply: 5)
  936. problems with Ghost cells - (reply: 1)
  937. bisulfite sequencing: cell lines vs tumors - (reply: 3)
  938. cell type specificity ? - (reply: 1)
  939. Passage number and behaviour of cell line - (reply: 10)
  940. Mycoplasma Testing in culture medium - (reply: 2)
  941. Anti Lamp-1 or Lamp-2 for COS cells? - (reply: 3)
  942. Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
  943. will the passage number affect the performance of THP-1 cells - (reply: 1)
  944. A drug to inactivate glial cells - (reply: 2)
  945. Duration of cell line transfected - (reply: 2)
  946. antibiotic dose response for suspension cell - (reply: 2)
  947. Emergent: Toxicity of cell lysis buffer :( - (reply: 2)
  948. Help with endothelial cells - Endothelial Cells Culture (reply: 4)
  949. Cell lysis for westerns? - Immediately after harvest?? (reply: 8)
  950. Quantity of genomic dna in human cells - (reply: 5)
  951. siRNA Stable transfection in human fibroblast cells - (reply: 6)
  952. Cell proliferation/viability assay - use of WST and 1-Methoxy PMS (reply: 4)
  953. Add L-glutamine in cell culture medium? - (reply: 5)
  954. Mycoplasma contamination - detection and de-contamination (reply: 7)
  955. transfection of Hek 293A cells - (reply: 5)
  956. cell scraper - (reply: 4)
  957. Trypsin does not work - need to detach monocytes (reply: 12)
  958. Transfection of cells by viral DNA - (reply: 4)
  959. neural progenitor cell death in multi-well plate - (reply: 10)
  960. Hybridoma Cell lines - How to do it (reply: 1)
  961. more contamination owes - spherical cell like contamination (reply: 8)
  962. C2C12 cells model for differentiation - (reply: 1)
  963. about glucose uptake test in hepatocytes - (reply: 2)
  964. Glycerol gradients - glycerol gradients for cell extract fractionation (reply: 2)
  965. Fibroblast and epithelial cells in tumor samples and primary cultures - (reply: 2)
  966. DNA Repair in different cells - (reply: 2)
  967. Getting crazy because of cell contamination - (reply: 27)
  968. lncap cell culture - (reply: 3)
  969. cell fractionation plus IP - (reply: 12)
  970. Lab dealing with factors affecting cell membrane permeability - (reply: 1)
  971. passaging 293T cells - (reply: 5)
  972. lysosomes in cells - (reply: 2)
  973. Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
  974. making more chemically competent cells - (reply: 6)
  975. Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
  976. anyone has EcR293 cells? - (reply: 5)
  977. Searching specific Cell line - (reply: 1)
  978. Help me....About the 3T3-L1 Cells - (reply: 2)
  979. Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
  980. HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
  981. How many cells (nuclei) per ml pellet? - (reply: 3)
  982. Glycyrrhizin (GL) and cell culture - (reply: 1)
  983. transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
  984. ES and MEF cell - (reply: 1)
  985. How to reduce cell clumping for immunocytostaining - (reply: 1)
  986. primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
  987. ATCC cell lines - How is your experience? (reply: 1)
  988. siRNA transfection of non-adherent lymphoma cells - (reply: 2)
  989. need help on learning cell and tissue culturing - (reply: 6)
  990. can I re-freeze the 4% paraformaldehyde for longer storage? - leave cells in 4% paraformaldehyde 4 FACS? (reply: 3)
  991. Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
  992. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
  993. How to grow adherent cells without attachment??? - (reply: 2)
  994. mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
  995. what's the best system to get siRNA into a cell? suggestions - (reply: 1)
  996. Lysis buffer - muscle cells lysis (reply: 1)
  997. Help with defective cell lines - (reply: 1)
  998. How to measure neuronal cell differentiation? - (reply: 1)
  999. Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
  1000. Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
  1001. Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
  1002. Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
  1003. Trypsin vs Non Enzymatic - (reply: 2)
  1004. transfection - selection of trransfected SH-SY5Y cells (reply: 3)
  1005. Who killed my cells??? - Toxicity during transfection (reply: 3)
  1006. Transfection reagent for HT-29 and HCT-116 cells - (reply: 4)
  1007. MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
  1008. FACS - permeabilization of cells without killing (reply: 1)
  1009. DC harvesting for FACS - dendritic cells (reply: 2)
  1010. about cell sonication - (reply: 2)
  1011. Subculturing cells grown in serum free media - (reply: 2)
  1012. How to screen mammalian cells clones? - (reply: 3)
  1013. Commonly used endothelial cells - (reply: 2)
  1014. aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
  1015. making homogeneous cell lysate suspensions - (reply: 1)
  1016. How to injure cell wall of chlorella for transformation - (reply: 2)
  1017. transfection of differentiated Caco-2 cells - (reply: 1)
  1018. Cell cycle (phase) analysis by FACS analysis - PI staining to gate different phases of cell cycle (reply: 1)
  1019. Mycoplasma - input needed! - Please help me by answering these questions! (reply: 6)
  1020. Cell attachment - cells and jam (reply: 1)
  1021. Immunofluorescence - cell surface protein detection - How do I detect only cell surface expressed prot. (reply: 3)
  1022. How to choose the flask shape and volume for Vero cells? - Is it possible to reduce? (high-cost reagents) (reply: 1)
  1023. cell lines and their particularities - where is the info (reply: 1)
  1024. Separation of dead cells from cultures - (reply: 8)
  1025. methods to 'quantify' morphology change in siRNA treated cells - (reply: 1)
  1026. How to avoid contamination of primary cells during preparation - (reply: 1)
  1027. RNAi in CHO cells - (reply: 5)
  1028. How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
  1029. Mold Contamination in cell culture - Mold and Cell Culture (reply: 7)
  1030. competent cells question - (reply: 3)
  1031. Growing cells on soft agar - MCF-7 cells on agar plates (reply: 1)
  1032. What the chance to get live cells? - (reply: 11)
  1033. Transfection of 293T cells on coverslips - (reply: 1)
  1034. Clumping of MCF7 cells - (reply: 3)
  1035. Cell specific Sonication - How different are cell types (reply: 1)
  1036. Cell counting - (reply: 2)
  1037. Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
  1038. Sonicating cell lysate for Westerns - (reply: 2)
  1039. has anybody done live cell staining? - (reply: 6)
  1040. Putting 2 plasmids in 1 e. coli cell - (reply: 4)
  1041. inducible stable cell line.... - (reply: 1)
  1042. assays to assess cancer cell growth? - (reply: 4)
  1043. Transforming DH5 alpha cells with pUC19 vector - Cloning (reply: 3)
  1044. Insect cell sonication protocol? - Protein Purification (reply: 1)
  1045. Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
  1046. best cell colture media for in vivo imaging - (reply: 3)
  1047. A question about cell stain: fix the cell have fusion protein - (reply: 1)
  1048. How to get rid of contamination in stable transfected cells - (reply: 3)
  1049. how can i culture virus in primary cell lines? - microbiology (reply: 1)
  1050. coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
  1051. can i freeze cell lysates for western blotting? - (reply: 3)
  1052. real-time RT-PCR one cell - (reply: 2)
  1053. Subculturing astrocyte from E15 days mouse embryo - Technique (reply: 2)
  1054. Lipof/Opti transfection cells died in DMEM/F12 - Transfection (reply: 4)
  1055. Why add phenol red in trypsin? - (reply: 14)
  1056. Cell cycle question - CKIs - (reply: 2)
  1057. Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
  1058. Definition of cell confluency - (reply: 6)
  1059. How to seed adhering cells (HepG2) evenly in 24-well plates? - (reply: 5)
  1060. Incubation - Effect of other cell lines during incubation (reply: 1)
  1061. growing epithelial cells from tumor - (reply: 2)
  1062. In Vivo cell proliferation - (reply: 3)
  1063. detecting Mycoplasma in cultures - (reply: 7)
  1064. Do you reuse cell culture vessels? - (reply: 3)
  1065. more help: cell culture problem - (reply: 1)
  1066. Isolation of mouse hepatocytes using Percoll - Protocol for isolation of mouse hepatocytes (reply: 3)
  1067. get metaphase cell by nocodazole? - (reply: 1)
  1068. Passage-hepatic stem cells - (reply: 1)
  1069. Establishment of primary cell lines - (reply: 1)
  1070. Fungal Infection in mesangial cells - Primary Cell Culture (reply: 2)
  1071. Freezing medium and cell counting - (reply: 6)
  1072. Tumor cells immortal? - (reply: 5)
  1073. "Cell culture discard in chloros (10,000) ppm" ? - (reply: 4)
  1074. cells transfection - transfecting DNA amounts (reply: 5)
  1075. human rTGFß for mouse cell culture applications? - (reply: 1)
  1076. Gene expression in mammalian cells - (reply: 6)
  1077. Thawing of competent bacterial cells - (reply: 2)
  1078. Origins of primate, canine, vertebrate cells, etc. - (reply: 1)
  1079. Tissue culture and cell culture? - (reply: 4)
  1080. Do all 293 cells contain an adenovirus? - Do all 293 cells contain an adenovirus? (reply: 3)
  1081. seeding of cells for transfection - (reply: 4)
  1082. Liver Perfusion - Hamsters Liver Perfusion and Hepatocytes isolation (reply: 2)
  1083. how to prevent HEK293 cells from coming off the plate - (reply: 4)
  1084. transfection problem---SOS! - why the cells should be split before transfection? (reply: 7)
  1085. Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
  1086. MDA-MB-231 cell culture - (reply: 7)
  1087. Preparation of competent cells of pichia - (reply: 1)
  1088. Problem in culturing PZ-HPV-7 cell line - (reply: 4)
  1089. Murine markers on hematopoietic stem cells (CD34, Sca-1, etc) - Looking to identify markers on megakaryocytic progenitors (reply: 2)
  1090. breast primary culture cells - (reply: 2)
  1091. filtering for cell culture stuff - filter cell culture (reply: 5)
  1092. transfection of single copy of plasmid per cell - (reply: 3)
  1093. looking for cell line: CEMX174(T1) - (reply: 2)
  1094. SF9 cells - (reply: 2)
  1095. Natural Killer cells - (reply: 1)
  1096. Stable Transfection in Sf9 cells - Stable Transfection in Sf9 cells (reply: 5)
  1097. B-gal staining of cells - (reply: 1)
  1098. Need HeLa cell extract prepartion protocol - wait your answer online (reply: 1)
  1099. bands from tissue one size, from cells - size doubles - help! (reply: 4)
  1100. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
  1101. red blood cell lysis - (reply: 6)
  1102. RNAi in Jurkat cells - (reply: 3)
  1103. Transfection - Cell death after transfection - (reply: 6)
  1104. FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
  1105. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
  1106. co-culture of two adherent cells - (reply: 4)
  1107. Cell lysis when isolating dendritic cells - (reply: 1)
  1108. hep-2 & Hep-G2 cell line - difference between hep-2 & Hep-G2 cell line (reply: 22)
  1109. Cell contamination, needs the help urgently - How to remove? (reply: 2)
  1110. EDTA only for cell dissociation - (reply: 7)
  1111. where to buy sterile grease used for isolate cell clone? - (reply: 2)
  1112. please recommend me a RNA isolation kit - RNA isolation from 100 tumor cells (reply: 1)
  1113. Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
  1114. RT-PCR of different cell lines - (reply: 1)
  1115. cancer cell culture - protocols on cancer cell culture (reply: 8)
  1116. Mammalian cell line - monolayer - monolayer growth of mammalian cell line (reply: 1)
  1117. How many cell passages to be safe ? - (reply: 8)
  1118. Contamination issue in cell culture - (reply: 109)
  1119. Visualizing Cell Pellet - (reply: 1)
  1120. LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
  1121. Stable transfection of S2 cells - (reply: 1)
  1122. ATDC5 cell line - i am looking for this cell line (reply: 2)
  1123. Dendritic cell -isolation ---viabillity is very low - Immunology (reply: 3)
  1124. measuring apoptosis after treatment of cells with ZVAD-FMK - (reply: 1)
  1125. Suspension Culture - Can it be transformed into adherent cells? (reply: 5)
  1126. Help! Cell staining problem - (reply: 3)
  1127. choosing insect cell for high-cystine protein expression - (reply: 1)
  1128. Cells in collagen gel - Growing mammalian cells in collagen gel (reply: 1)
  1129. H295R Cell transfections - (reply: 2)
  1130. cells in the earth - (reply: 4)
  1131. Trypsin free detachment?? - (reply: 2)
  1132. Freezing and thawing of cells - (reply: 112)
  1133. Human Macrophage cell line - quick help please - (reply: 3)
  1134. cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
  1135. Primary epithelial cell line - Isolation of epithelial cells (reply: 3)
  1136. CaPO4 transfection prevents trypsin activity - Trying to split after transfecting (reply: 1)
  1137. single cell PCR - (reply: 8)
  1138. Transfection using PEI - Transfection of 16HBE and CFBE cells (reply: 4)
  1139. Tet-responsive cell line - cells cannot become confluent (reply: 1)
  1140. 293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
  1141. What are the cells comprising PBMC?? - (reply: 1)
  1142. MC57G cells and transfection - (reply: 1)
  1143. transfection efficiency - mammalian cell transfections (reply: 1)
  1144. pBABE Comp. Cells - Special cells for transformation (reply: 3)
  1145. Any ideals for cell fragmentation? - (reply: 2)
  1146. cell lysis - How to lysis cells (reply: 2)
  1147. DMSO and cell cycle - (reply: 2)
  1148. cell culture RNA extraction - cell culture RNA extraction (reply: 4)
  1149. Origami B cells grow too slowly!! - (reply: 1)
  1150. about LNCap cell lines - (reply: 1)
  1151. CBP expression problems in mammalian cells - (reply: 1)
  1152. problem by using percoll to purify primary cell - (reply: 1)
  1153. the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
  1154. T cell proliferation - problems (reply: 1)
  1155. lymphocytes' RNA extraction - How many cells (reply: 1)
  1156. Selection of cell ine - (reply: 2)
  1157. Cell culture problem - weird stuff grow in my cells - (reply: 6)
  1158. Cell counting of Strongly adherent cells - (reply: 4)
  1159. How to detect drug's cytotoxcity by MTS - (reply: 2)
  1160. How to assay the amount ofcollagen on cell/scaffold composites? - (reply: 1)
  1161. PBMC-B cell - (reply: 1)
  1162. Plant Culture Cell - (reply: 1)
  1163. stable cell line - stable cell line (reply: 1)
  1164. Cell line for glutamate receptors - Glutamate receptor cell lines (reply: 1)
  1165. lipofectin mediated transfectionof HepG2 cells - (reply: 1)
  1166. stable transfection of epithelial cells - stable transfection in mammary breast cell lines (reply: 8)
  1167. Shipping Cell Cultures - Shipping Cell Cultures (reply: 3)
  1168. using culture sup of one cell line  to induce 2nd cell line - (reply: 1)
  1169. Stable Trasfection into Hek 293and Hela cells - (reply: 1)
  1170. Isolation of nuclei from mammalian cells - (reply: 1)
  1171. Re: how to seperate deadsuspension cells from alive ones - (reply: 1)
  1172. mutation in cancer cell line - (reply: 1)
  1173. Reviving Cells from Liquid Nitrogen - (reply: 2)
  1174. cell lines for anti-inflammatory study - (reply: 1)
  1175. How to tell different cellstypes apart - (reply: 2)
  1176. Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
  1177. Centrifuge cell at room temperature? - (reply: 1)
  1178. cells washed away - cell culture problems (reply: 6)
  1179. cell viability/toxicity - (reply: 3)
  1180. Stable Transfection of CEM cells - (reply: 1)
  1181. seperation of epithelial cells and fibroblast from a biopsie - a culture of epithelial cells is needed without fibroblasts (reply: 1)
  1182. total protein assay in cell extracts - (reply: 3)
  1183. Labeling cells with AT32P - cell biology (reply: 1)
  1184. how the seperate dead cells from living ones. - (reply: 5)
  1185. animal cell culture - (reply: 2)
  1186. distilled water shock of red blood cells - (reply: 4)
  1187. please help me - how to extract cd3 protein from jurkat cell (reply: 1)
  1188. Influenza inoculation of Hek293 cells - (reply: 1)
  1189. Quantifyng Mycobacterium Cells - (reply: 5)
  1190. Growth conditions for T cellline - (reply: 2)
  1191. Mouse B cell Activation - (reply: 1)
  1192. Cell isolation form cord blood - (reply: 1)
  1193. Is there any simple  way totell a cell coming from cell division - (reply: 1)
  1194. T cell isolation from rat wholeblood - (reply: 2)
  1195. Pituitary cell culture - (reply: 1)
  1196. Whole Cell PCR from BacteriaProtocols? - (reply: 4)
  1197. collagen measurement andisolation in cell based - (reply: 1)
  1198. genomic and fragmented DNAextraction from human  cells.  Ple - (reply: 1)
  1199. Units of trypsin? - (reply: 1)
  1200. RNA isolation from a single cell - (reply: 1)
  1201. Competent Bascillus Cells - (reply: 1)
  1202. cell lysis - How do I decide what solution to use? (reply: 2)
  1203. adenovirus infection (293 cells)protocol - (reply: 1)
  1204. cell line with deletion in mitochondrial DNA - Molecular Biology (reply: 1)
  1205. Cell senescence markers - (reply: 1)