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Cell culture Related Discussions
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cell lysate colorless? - (reply: 1)
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Does anyone else gas their cells like this?! - (reply: 5)
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Transient transfection trouble- massive cell death - (reply: 1)
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animal cell lysis, on plates - (reply: 2)
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selection of transfected cells - (reply: 1)
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Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3)
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How to count cells on plate - (reply: 3)
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Transformation of non-competent E. coli? - Any shortcut for preapring competent cells? (reply: 2)
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boosting expression in stable cell line? - (reply: 5)
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too few cells used for electroporation/ transfection - (reply: 4)
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any way to inactivate trypsin without using FBS? - (and still let cells survive ^__^) (reply: 3)
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Quantification of VEGF peptide levels in human breast cancer cells. HELP! - (reply: 6)
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making Stocks of HL 60 cells - making Stocks of HL 60 cells (reply: 1)
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Mycoplasma contamination. - (reply: 6)
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cell and tissue culture - healthy test (reply: 1)
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Need human cells for a science fair project - (reply: 4)
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THP-1 cell culture - (reply: 3)
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Mammalian expression problem - HEK cells expression (reply: 3)
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Contamination and its fungus - persistant fungal contamination in cell cultures (reply: 1)
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Removing all cell surface proteins/receptors - (reply: 3)
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The action of alcohol on bacteria cells - Washing bacteria cell (resuspending) pellets with methanol? (reply: 2)
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PBS for releasing anchored cells, what kind? - (reply: 2)
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Which part of the kidney are COS-7 cells from? - (reply: 1)
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Chemical transformation in 96 well MTP - How to make competent cells in MTPs (reply: 2)
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Do COS-7, CHO-K1 and LLC-PK1 cells express dopamine receptors? - I need an answer soon... (reply: 2)
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Is it hard to make knock-out cells/mice? - (reply: 4)
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trypsin - wat is the source of trypsin we use in cell culture (reply: 1)
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Competent Cells - (reply: 1)
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PC12 cells do not attach on collagen - (reply: 4)
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cell fractionation and low protein concentration in cytosol - (reply: 1)
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IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
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Adding some PBS to cells - (reply: 3)
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Quantifying Cell Lysis or the Presence of DNA - (reply: 3)
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What exactly are CD4+3- inducer cells? - simply a specific form of "T-helper-cells"? (reply: 2)
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In vitro toxicology test by animal cell line - Looking for methods (reply: 1)
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inoculating liquid media from frozen cell stock ok? - (reply: 6)
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Cell treating - Do you... (reply: 4)
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cell lysis - (reply: 3)
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How to fixate cells onto glass microscope slide - (reply: 2)
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Is there any destructive effect on nucleic acid when harvest the cell by scrapin - (reply: 1)
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Transfected copies per cell - (reply: 1)
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RAW 264 cells - (reply: 1)
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How to concentrate retrovirus produced by Phoenix-Ampho cell - (reply: 4)
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Have I killed my cells? Doh! - (reply: 3)
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Looking for specific cancer cell line - Need a parent line with metastatic and non-metastatic lines (reply: 2)
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Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
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mesenchymal stem cell differentiation into osteoblasts (alizarin) - (reply: 2)
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Troubles in transfection methods! - All cells die before making a selection (reply: 5)
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siRNA into RAW264.7 cells? - any ideas (reply: 4)
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Heat-shock treatment problems with adherent cells - (reply: 1)
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No Retrovirus using the Phoenix - Ampho cells - (reply: 1)
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IEC-6, IL-6 and LPS - Cannot get my IEC-6 cells to secrete Il-6 (reply: 2)
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Creating stable cell lines - (reply: 4)
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Cholesterol Assay - Commerically available cholesterol assay kit for cell extracts (reply: 1)
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Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
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Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
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cell culture question - passage number - (reply: 5)
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A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
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How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
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293t Cell Attachment Issues - (reply: 7)
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giant cells? - (reply: 6)
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Direct Cell Lysis in Laemmli - (reply: 1)
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Cell Morphology - (reply: 1)
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When to use PMSF in a cell lysis buffer? - (reply: 9)
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staining for receptors in cell line - (reply: 1)
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Counting B cells in hemacytometer - (reply: 2)
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Coating cell dishes with peptides - (reply: 2)
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Does it look like cell clumps/artefact/contamination? - (reply: 3)
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What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
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what cell line to use for visualizing membrane localized protein - (reply: 1)
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Define cell passage number - (reply: 2)
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THP-1 cells from Sigma - (reply: 1)
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DNA "snot" in cell lysis/protein extraction - (reply: 6)
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DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
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H.influenzae and S.pneumoniae cell culture - H.influenzae and S.pneumoniae grow on plates but not in broth (reply: 1)
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Cells die after changing medium - Is there role of FBS? (reply: 1)
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Different cell culture plastics, problems with cell adherance - (reply: 6)
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transfecting large gene in mammalian cells - difficulties in transfecting large gene in mammalian cells (reply: 5)
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Comparing surface protein expression of different cell lines - (reply: 1)
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How to compare the fluorescence staining signal in cells - (reply: 6)
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what cells to use for expression of pGEX3? - (reply: 1)
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What's "polarized T cell"? - (reply: 1)
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SMP30 silencing in vivo - stem cell biology (reply: 2)
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Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
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p53 status in cell lines - (reply: 3)
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Cell Adherence - (reply: 2)
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How to kill bacteria in treated cells? - (reply: 6)
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cell line to use for neural regeneration? - (reply: 1)
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Does immortalization affect non-dividing short-living cells? - (reply: 3)
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Why do viable cells take up DAPI? - DAPI must only stain non-viable cells (ICC) (reply: 7)
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Quenching oxygen in cell culture - (reply: 1)
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what is trypsin assay by BAEE - (reply: 2)
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Are these cells still viable? (Trypan Blue Stain) - Using this stain for the first time against Sf9 cells (reply: 6)
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Trypsin Assay Calculation - Please Help me!!Really in urgent (reply: 4)
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Ratio between cells and lysis buffer - (reply: 1)
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Immunofluorescense of floating cells - How to IF the floating cells during apoptosis? (reply: 4)
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What cell lines can be used to run an anti-angiogenic assays? - (reply: 1)
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NK Cell Isolation - negative selection kits - (reply: 1)
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Cell count in solution - (reply: 2)
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Using TrypLE as alternative for trypsin - in cell isolation from tissues (reply: 3)
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Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
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cell cryopreservation in -80 - (reply: 2)
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Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
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Cell growth inhibition by lipofectamine 2000 - (reply: 1)
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T cell activation for intracellular cytokine detection by Flow Cytometry - (reply: 1)
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Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
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Cell death after PMA treatment - (reply: 5)
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what protease is best for primary cell culture - (reply: 1)
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Basic immuno/histo common knowledge question - Where can I find what cells in what organ? (reply: 4)
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Help MCF7 , MDA231 cells - Treatment of cells. (reply: 1)
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How does invitro morphology change w/cell cycle - (reply: 1)
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protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
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DNA extraction...help needed! - extracting dna from buccal cells in ethanol (i think) (reply: 5)
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Cell Clumping - (reply: 1)
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Help with clonogenic survival assay for PC3 prostate cancer cell line - HELP! (reply: 5)
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Competent cells for transformation - (reply: 1)
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How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
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Mycoplasma - How to detect and treat mycoplasma contamination in culture and RNA? (reply: 9)
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Post-doc position available in my lab - Leukemia stem cells (reply: 4)
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Is it a general effect that stably transfected cell line has lower cell prolifer - (reply: 4)
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how can i seed 0.5 cell /well? - (reply: 10)
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RPM for Pelleting down cells - (reply: 3)
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how to culture cancer stem cells? - help (reply: 1)
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Staining suspension cells for microscopy - (reply: 1)
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Liver cancer primary cell culture - urgent!Thanks (reply: 1)
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Umbilical cord blood stem cells - (reply: 1)
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Cell lysate preparation - (reply: 2)
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Cell ELISA, Permeabilization cell - (reply: 1)
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Cell cycle analysis. - Cell synchronized or no-synchronized. (reply: 6)
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express toxic protein in insect cells - (reply: 2)
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Any commercially available primary cells? - Need some cardiomyocytes (reply: 1)
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two bacteria populations observed when there should be just one - how to get rid of cell doublets? (reply: 4)
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help with making ultra competent cell (Inoue method) - (reply: 3)
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How to seed less number of cells - (reply: 2)
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"Twin-peak" @ G2/M phase? - funny result of cell cycle assay (reply: 1)
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Glioma cell lines - (reply: 2)
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Storage of Cell pellet - (reply: 8)
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Intracellular proteins on live cells - (reply: 3)
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making a stable cell line - (reply: 3)
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siRNA transfection in MLE12 cells - (reply: 3)
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Problem in growing DG44 CHO cells - (reply: 2)
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making stable cell line - (reply: 5)
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GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
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Cell culture basic questions - Basic questions on cell culture (reply: 7)
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flourescence in non-transfected cells! - (reply: 4)
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% confluency question - when to transfect the cells? (reply: 2)
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Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
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Free grid image for counting cells (freeware) - simple but useful (reply: 7)
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Cell toxicity and color - (reply: 1)
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PCR and competent cells - (reply: 3)
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Nuclear extracts from myeloid cells? - (reply: 9)
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primary Hepatocytes - experts advice - (reply: 1)
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siRNA for retinal pigment epithelial cells - (reply: 1)
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high levles of cell death in PC12 cells - (reply: 3)
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PMA - used to differentiate THP-1 cells - (reply: 6)
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Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
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tranwells and C6 glioblastoma cell migration - (reply: 1)
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Cell culture contamination - (reply: 9)
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yeast cell cycle and temperature - FACS profile at 25 and 36 (reply: 1)
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Melanin extraction from melanoma cells, How? - (reply: 3)
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Strange black particles while growing Sf9 insect cells - (reply: 3)
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I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
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FRET Imaging :Hela cells - (reply: 2)
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Jurkat Cells and Proliferation Assay - Click-iT EdU - (reply: 2)
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cell density - (reply: 2)
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3-D tissue model for endothelial cell culture - Looking for any in vitro 3-D models to grow endothelial cells to measu (reply: 1)
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How to kill yeast cells :) - chloramine or autoclaving (reply: 19)
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RNA isolation from small amount of suspension cells - (reply: 3)
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Culturing THP-1 cells, they grew too slow...Need help... - (reply: 11)
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cell migration - (reply: 1)
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Crystal violet to determine cell numbers - (reply: 1)
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Do RAW cells (high passage number) lyse in PBS? - (reply: 2)
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red blood cells and the HA Assay - differences between species (reply: 6)
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How much media needed for cells in different cell culture plates - (reply: 3)
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Cell separation - Cell separation (reply: 1)
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Very high confluence after thawing cells– good or bad? - (reply: 8)
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Cell Proliferation inhibition - arrest? (reply: 4)
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Jurkat Cell Adhesion - Techniques to make Jurkat Cells to adhere to plastic surfaces (reply: 2)
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Cell clumping in culture: Folic acid or i-Inositol? - (reply: 3)
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count cell number - (reply: 3)
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Could the strange G418 resistance fo my Chinese hamser cell lines be due to the - CAN I USE UNTREAED plates for selection? (reply: 2)
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stable cell screen - (reply: 2)
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Are adherent THP-1 cells macrophages? - (reply: 1)
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Microscope Settings - Microscope Settings for Trypan Blue Exclusion Assay (reply: 1)
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siRNA transfection in endothelial cells - (reply: 3)
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Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
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IPTG induction - at what cell density (reply: 5)
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whole cell sample vs cell lysate - (reply: 2)
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siRNA plus drugs - weird cell morphology (reply: 3)
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Is it Okay to provide 5%CO2 to insect cells SF21? - (reply: 4)
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identify cell lines - (reply: 4)
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Does Trypan Blue expire? - (reply: 3)
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RNA isolation from small amounts of cells - (reply: 1)
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DNA extraction from PFA fixed cells - (reply: 2)
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Help of establishing stable cell line - (reply: 1)
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Cell lysis method for running cell lysate on Western blot - (reply: 4)
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Problems with making efficient chemically competent cells - (reply: 2)
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N in Cell Culture - What is the proper way to determine your "n"? (reply: 2)
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Can cell debris move? - (reply: 4)
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MLN cell isolation....Help! - (reply: 1)
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Pansorbin cells - (reply: 3)
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double transfection plus siRNA - in HEK293 cells (reply: 1)
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cell line culture - (reply: 2)
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Chromosomal aberration assay for ES cells - (reply: 1)
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Unexplained Cell Culture Death - Expertise needed (reply: 7)
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transfecting siRNA into primary fetal mouse hepatocytes - (reply: 6)
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New to Cell Culture and need some basic information - help needed (reply: 12)
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HEK 293T cells - problems getting proper attachment. - (reply: 9)
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Transfection of 293-T cells in 6-well plate, using PEI - (reply: 4)
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plentivirus - stable cell line (reply: 1)
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Cell Culture Question - (reply: 3)
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General procedure for passing (splitting) cells - (reply: 3)
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What's a good Nuclear extraction kit for primary cell? - (reply: 1)
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Establishing thymoctyes cell lines - (reply: 1)
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yeast specialists - strange behavior in yeast cells (reply: 9)
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media or cell problem? - (reply: 5)
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quenching of GFP after selection of stable cells - (reply: 1)
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stable cell line, but quenched GFP? - (reply: 5)
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PCR on Genomic DNA from cell lysate - (reply: 5)
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dermal multipotent cells - (reply: 1)
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cell line contamination - what to do? (reply: 7)
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contamination in MACS seperated cells - (reply: 3)
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multiple plasmids in cells - (reply: 1)
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Fungal contamination in cell lines! - (reply: 2)
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Can anyone tell me why my cells are dying? - My mouse embryonic stem cells are dying and I'm baffled as to why. (reply: 2)
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siRNA transfection in primary hepatocytes - (reply: 1)
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Loading cell pellet in SDS-PAGE - (reply: 4)
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What happen to my NIH3T3 cells? - (reply: 6)
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perovskite oxide nanowires in living cells - (reply: 1)
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Really, isn't there anyone who can recommend a TUNEL assay? - for adherent cells using fluorescent microscopy? (reply: 2)
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Fixing cells in wells? - (reply: 4)
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Problem with thawing cells from liquid nitrogen? - (reply: 6)
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Problem with mouse embryonic stem cells - contamination? - (reply: 1)
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Cell immortalize - (reply: 2)
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TUNEL assay- should I grow cells on slides? - Or label cells in suspension then deposit on slides? (reply: 1)
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confirming how the gene causes decreased growth/increased cell death - (reply: 4)
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To keep dead cells when harvesting? - (reply: 2)
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anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
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how to sdudy the matter exchanges among cells? - (reply: 4)
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RAW 264.7 cell transfection - Probs transfecting RAW cells with lipofectamine LTX (reply: 2)
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Transfecting P19 embryonic carcinoma cells - (reply: 2)
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Transfecting NS0 cells - (reply: 5)
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Calculating cell purity after MACS enrichment - (reply: 1)
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Role of Trypsin in Hep G2 culture - (reply: 1)
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Chemoresistance assay - How to measure the chemoresistance of cancer cells (reply: 6)
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is a matrigel layer a 3d cell culture? - (reply: 6)
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how to cryopreserve HepG2 cells at -80 degrees? - (reply: 6)
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hemin in cell cultures - (reply: 8)
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Cells not sticking to chamber slides after fixing - (reply: 8)
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Amount of Cell Lysate for Western Blot - (reply: 2)
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stable transfected cell line in absence of G418 - (reply: 6)
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N2a cells differentiation - (reply: 4)
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Tris and cell toxicity - (reply: 4)
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cell differentiation and survival - (reply: 4)
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DNA laddering in Smooth Muscle Cells - (reply: 1)
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Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
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EGFP stable cell line proof - (reply: 3)
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Does sonication of cell lysate.... - (reply: 2)
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Stem Cell Culture Medium - Stem Cell Culture (reply: 1)
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rat bone marrow mast cell maturation - Anyone have any experience or advice? (reply: 1)
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Cell Viability Dyes Better than Trypan Blue - Trypan blue vs Erythrosine B vs Other? (reply: 4)
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Does formaldehyde crosslink completely kill cell? - (reply: 2)
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HeLa cells - safety concern - (reply: 2)
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bc cell differentiation - T47ds (reply: 1)
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Where to buy gastric parietal cell lines? - (reply: 1)
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Morphology of ES cell clones - Does this clone look like ES cells? (reply: 4)
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Can any one help to transfect these cells ? - SCN neuron transfection (reply: 2)
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cell counting again - (reply: 2)
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How to send cells for immunfluorescent staining? - (reply: 8)
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Cells dying upon temperature shift. - (reply: 7)
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Spermatogonia stem cell culture - (reply: 4)
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THP-1 cell culture problem: Cells die after thawing - (reply: 4)
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Fixation of cells for long time storage - (reply: 1)
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cell migration, cell invasion and cell proliferation assay - (reply: 5)
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cell culture - problem with suspension CHO cell revival (reply: 2)
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Optimization of seeding cells number for MTT assay - (reply: 5)
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MCF-7 cell grow too slow - (reply: 5)
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Living cell GFP imaging - (reply: 6)
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weird S2 cells - (reply: 1)
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neuronal cell suspension from brain - (reply: 4)
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Transfection of Drosophila cell culture - Which is the best transfecting agent? (reply: 1)
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A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
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Centrifugation speed for adherent cell line - (reply: 11)
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time required for cell recovery after thawing - (reply: 8)
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Competent Listeria Cells - (reply: 3)
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Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
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Protein extraction from animal cells - (reply: 7)
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Sepration of cells from cell debris in sample - (reply: 2)
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Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
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HBSS vs PBS to deliver compounds to cells - (reply: 3)
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centrifugation speed for fixed cell - (reply: 2)
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competent cell contamination? - (reply: 3)
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Lysis of fat cells/adipocytes - (reply: 7)
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CHO-S cell - (reply: 1)
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cell lines - (reply: 2)
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On trypsin neutralizing solution - (reply: 2)
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Drug treatment enhanced cell growth? - (reply: 1)
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problem with transformation - ligation and cells seems to be fine (reply: 3)
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Wanted! Protocol Immunocytochemistry with HRP-DAB reaction - Need Non-fluorescent immunostaining on cultured cells (reply: 2)
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dead cell staining followed by fixation? - (reply: 4)
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can hyaluronidase be used to detach/digest cells? - (reply: 1)
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Cell Suspension for MRI Study - (reply: 3)
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Search more efficient transfection reagent for BJ cells - (reply: 1)
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Unexplained cell death - (reply: 6)
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Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
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what's wrong with my MTT assay on a suspension cell line - (reply: 8)
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Ooops! I messed up! Help! - freezing cells (reply: 5)
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Co-IP in sonicated whole cell lysates - (reply: 3)
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no GFP in cells - (reply: 7)
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SLOW cell growth - (reply: 2)
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Apyrase in cell culture - Half-life? (reply: 3)
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Cells are lost after fixation - (reply: 14)
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primary sertoli cell transfection - (reply: 2)
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how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
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Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
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Hela cell contamination? - (reply: 10)
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Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
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brdu staining on gfp+ve cells - (reply: 1)
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How to infect T cells? Lentiviral or retroviral? - (reply: 2)
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dendritic cells culture - (reply: 3)
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cell lysis and save the ECM - (reply: 1)
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Harvesting cells following transfection - Scrapping or trypsin (reply: 2)
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Competent cells not growing - (reply: 5)
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preparation of bacterial cell lysate - how to do? (reply: 1)
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Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (reply: 3)
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Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
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Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
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Cell fractioning in order to perform binding assay - (reply: 1)
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competent cell didn't grow in LB - (reply: 15)
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BrDU staining on GFP positive cells - (reply: 1)
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Contamination or not? - Cell Culture Contamination (reply: 1)
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protocol for Trypan Blue exclusion assay - How to do trypan blue exclusiona ssay on adherant cells? (reply: 3)
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Infection of human cell lines grown in suspension? - Infection protocols (reply: 3)
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Need advice for growing THP-1 cells - (reply: 4)
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Live cell nuclei immunostaining - (reply: 2)
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Does a B lymphocyte contain lysosome inside? - Cell Structure (reply: 1)
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Cell fractionation-help! - (reply: 6)
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Nystatin makes vero cells round, too high concentration? - (reply: 3)
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How to separate cells in urine for FISH? - (reply: 1)
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CaCl2 Competent Cell - (reply: 3)
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Does trypsin digest integral plasma membrane proteins? - (reply: 2)
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passage of cell culture and protein locolization - (reply: 1)
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ChIP assay using Matrigel-3D cell culture - (reply: 2)
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quantifying my gene expression in 5 different cell lines - (reply: 1)
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Counting cells using ELISA method? - Counting cells using ELISA method (reply: 1)
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trypan blue - (reply: 2)
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culture CD34+ Hematopoietic Stem Cells - (reply: 2)
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Preparing cell lysate from cell culture for Western blot - (reply: 4)
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How to introduce DNA to animal cells? - (reply: 3)
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MTT assay + suspension cells - (reply: 4)
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Western Blot of cells in Laemmil - (reply: 5)
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AML cell lines - Where can I get them?? (reply: 1)
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prepare electrocompetent cells - (reply: 4)
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CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
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plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
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Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
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Just how many cells are lost when changing tubes? - (reply: 5)
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Cell cycle: ModFIT vs. FlowJo - diferent percentages outcomes on same histograms (reply: 1)
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problem with plating adherent cells in 24-well plates - (reply: 11)
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Raji Cell Histone Immunocytochemistry and Confocal Help - (reply: 1)
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Cell Seeding and Drug treatment - (reply: 12)
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Embryonic stem cell culture - (reply: 4)
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cell biology video clips - (reply: 2)
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cell seeding confusion - what does it mean (reply: 1)
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Using Adenovirus to transfect cells - (reply: 5)
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Cell assay for ROS measurment - (reply: 1)
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Red Cells in Clear Ficoll Layer Following Density Centrifugation - Monocyte Isolation (reply: 2)
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preparation of electroporation competent cells from chemically competent cells - (reply: 3)
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cell culture - passage number (reply: 7)
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do the competen cells chang the U to T - (reply: 4)
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increased BrDU incorporation but similiar cell cycle profile by PI or DAPI - (reply: 2)
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help! cell culture contamination too! - (reply: 5)
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recommendations for a viral titer cell line - (reply: 3)
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Subculturing primary human fibroblasts - Seeding density? (reply: 1)
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Experiment with KATO III cells - (reply: 1)
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SiRNA in MIN6 cells, nucleofection - (reply: 1)
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Cell passage number for non-cancerous cells - (reply: 2)
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Cell death with DMSO treatment, during transfection using polybrene - (reply: 2)
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stable transfection high expression - problems with low expression in stably transfected cells (reply: 1)
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Homework question - what happens to cells when they're put in distilled wate - (reply: 3)
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looking for protocol using DAPI for cell cycle analysis - (reply: 1)
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Synchronization of NIH 3T3 cells - (reply: 2)
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difference between staining vital cell and dead - (reply: 9)
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Question making competent bacterial cells - (reply: 2)
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Cell Growth Following Confluency - (reply: 2)
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Transfecting primary cells in suspension - (reply: 4)
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primary colonic epithelial cell culture - (reply: 1)
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trypsin concentrations for detaching cells - (reply: 2)
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storing unstained cells overnight for FACS next day - (reply: 3)
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Cell adheison assy on round surface - (reply: 1)
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Effectively washing adherent cells? - how do I optimize washing? (reply: 3)
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Formaldehyde cross link after washing cells - (reply: 2)
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cell synchronization - how to make cell stay at G0 phase (reply: 1)
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lysing cells on matrigel - (reply: 1)
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Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
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extractin RNA from cells grown on Matrigel - (reply: 1)
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siRNA stable cell lines - (reply: 6)
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Best compotent cells - (reply: 5)
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cell density for infection with baculovirus - what is the optimal cell dentisty at the point of infection (reply: 1)
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Creating Cell suspension - (reply: 1)
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pyridine, chloroform and metahnol as a solvent for chemopreventive agents - Is there anyone who has used these solvent for cell culture experiment (reply: 6)
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cell culture contamination - after transfection (reply: 15)
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How to prepare my own competent cells? - as above (reply: 5)
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minimum amount of cells used for western blot - (reply: 5)
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Is it possible to transform competent cells with linear DNA? - (reply: 5)
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natural killer cells - (reply: 2)
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Problems obtaining stable transfected 293 cells - (reply: 1)
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Help with different cell fixatives - Neuronal Fixation (reply: 7)
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Stable transfection of H295R cells - (reply: 3)
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tranfection of 3T3-L1 cells post differentiation - (reply: 2)
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stainig of cells for FACS - (reply: 2)
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pBABE-puro in 293T cells (not PhoenixA) - (reply: 5)
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stable transfections using SCC 9 cells - (reply: 5)
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Stable transfection of human adrenocortical cancer H295R cell line - (reply: 1)
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any tips for detecting phospho-ptotein in fixed adherent cells by flow cytometry - (reply: 2)
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293T cells: how to sort it the problem of detaching after washing with PBS - (reply: 3)
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How to seed adherent cells evenly in 150mm dishes? - (reply: 12)
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PMA induced apoptosis in HEK cells - (reply: 4)
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Why different mammalian cell lysate preparation methods? - (reply: 6)
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cell lysis to investigate phosphorylated protein - (reply: 1)
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centrifugation speed for mouse neural stem cells - centrifugation speed for mouse neural stem cells (reply: 3)
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cy5 cell tracker dye - (reply: 2)
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transformation competent cells problem - fastbac vectors (reply: 2)
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Mortality with Lipofectamin RNAiMax and RPE-1 cells - (reply: 8)
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transformation of competent cells - (reply: 2)
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Test for drug dose response in suspension cells before transfection - With Suspension cells, how do people distinguish difference between de (reply: 1)
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adipose stains? - specific stain to positively ID adipose cells (reply: 2)
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Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
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isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
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SURE E. coli strain - How make competents cells? (reply: 4)
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APC isolation from the Liver - How you can isolate the APC cells from the mice's liver (reply: 2)
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Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
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differentiated PC12 cells detatching - (reply: 3)
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Thp-1 cell transduction - (reply: 4)
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HIV-2 and MTT assay, cells lines to use - (reply: 2)
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tissue preparation for human/mouse islet cells - (reply: 1)
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Protein Extraction from cell media for western blot - (reply: 3)
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cell adhesion to plastic - (reply: 3)
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Can I use a cell free extract on Mass Spec? - (reply: 8)
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Chemically competent cells? - (reply: 3)
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cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
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bath sonicator for cell lysis - (reply: 2)
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serum starvation - induce cilia by serum starvation in Rpe-1 cells (reply: 7)
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Keep growing cells after an MTS/PMS assay - (reply: 1)
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RNA in HeLa cell - amount of RNA in a HeLa cell (reply: 2)
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Cell fractionation: nuclei do not pellet - (reply: 2)
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Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
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lentiviral transduction of suspension cell - (reply: 1)
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cell counting dilemma - (reply: 1)
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lentiviral transduction of suspension cell - (reply: 1)
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Immunostaining on bacterial cells? - (reply: 1)
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any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
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FACS sorting affects cell function? - FACS sorting affects cell function (reply: 2)
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cell counting calculation.... - (reply: 3)
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Phoenix 293T packaging cells - (reply: 3)
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suparnatant, cell free extract, crude extract - (reply: 4)
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competent cells transform 12 kb plasmid,urgent - (reply: 8)
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Whole cell extract on SDS/PAGE - (reply: 9)
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Making competent cells from current commercial stocks - (reply: 3)
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Calculation cell in 96 well plate - (reply: 1)
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G418 and resistant cells - can't kill the cells (reply: 8)
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MIN6 cells - (reply: 9)
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cell signaling & choice of the cell - (reply: 3)
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hard to transfect cell lines - (reply: 1)
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How can I separate nuclei from cytoplasm in trophoblastic cells - (reply: 1)
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salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
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Primary culture - Transparent cell? (reply: 2)
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transfection of chick cell - (reply: 3)
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non-adhernt cell washing - (reply: 1)
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Immunology on fixed bacteria - What are the basics of fixing cells for immunological analysis? (reply: 1)
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transfection of PC12 cell line - (reply: 1)
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Assaying apoptosis on non-detaching cell culture plates - which plates? (reply: 1)
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does anyone know how to prepare cell extract for Metabolic and Enzymatic Assays - (reply: 6)
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What is the right shape for NRK cells - (reply: 1)
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Why fix cells? - Advantages-Disadvantages (reply: 14)
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Please recommend a colon cancer cell line - what is the widely used colon cancer cell line? (reply: 3)
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cd3- and cd8+ cells - flow cytometry cell population question (reply: 4)
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confocal microscope, my cells die! - URGENT. (reply: 8)
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transfection with PA-1 cells - (reply: 1)
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How to separate the antigen presenting cell from spleen of rat - (reply: 1)
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Transfection of T47D cells with FuGene in 10cm plates - (reply: 2)
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Immunofluorescence staining of spheroid cells - (reply: 3)
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How small cells can Flow cytometry detect? - Flow cytometry to detect a small cell (reply: 2)
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tg1 and hb2151 cells - (reply: 2)
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How to avoid central cell seeding in 6-well plates - (reply: 19)
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Same cell line but different growth rate - (reply: 4)
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Fixing Cells for Flowcytometry - Glutaraldehyde, Formalin, Microwave (reply: 11)
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estimating number of cells to seed - (reply: 2)
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cell dead after changing the medium to PBS buffer - (reply: 3)
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PC12 cell culture. Do I have to coat plates first? - (reply: 2)
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Can scintillation fluid be used to harvest adherent cells? - (reply: 1)
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Difference between cell invasion and cell migration - (reply: 4)
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too many dead cells why? - (reply: 3)
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Do cells transfected with lentivirus express specific unique surface markers - (reply: 2)
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Help: Cell Lysing - (reply: 5)
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Avoiding cell clumps - (reply: 7)
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can cells just stop having the gene? - (reply: 4)
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how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
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question about dendritic cells and antigen-specific T cells - (reply: 1)
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CD3 and CD28 T cell stimulation in TUBES - Technical question (reply: 4)
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cell wall protein extraction of gram-positive - (reply: 1)
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How to do the lysis of cells without white stuff? - (reply: 1)
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cell culture - a beginner - (reply: 3)
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any video or animation of experiments about cell and molecular biology - (reply: 1)
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how to calculate cell number? - (reply: 1)
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Flow Cytometry 2 Parameter Statistics, please help - Can you find standard deviation of % Positive of Total cells based on (reply: 1)
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cell concentration calculation - (reply: 2)
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isolating of mouse spleen Dendritic cells by nycodenz - (reply: 4)
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Suspension cell culture. - (reply: 5)
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PC12-Neurite out growth - less no of cells with neurite (reply: 4)
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p53 independent cell death - (reply: 2)
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Dendritic cell line media - Dendritic cell line media (reply: 1)
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Assay for cell purity determination - (reply: 2)
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fungal contamination - fungal in cell culture (reply: 4)
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Double-Thymidine block of Hela cells - (reply: 3)
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endothelial cell isolation from mouse - using magnetic dynabeads from DYNAL (Invitrogen) (reply: 6)
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OD600 and cell density correlation? - (reply: 1)
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T cell activation via CD3 and/or Cd28 need to solve a problem - T cell activation cd3 (reply: 5)
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Plant cell culture- How do prevent differentiation of callus cells - (reply: 2)
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Confocal microscopy with primary cell cultures - (reply: 8)
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stable cell line characterization and validation - (reply: 1)
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Trouble concentrating cell extract -please help! - (reply: 9)
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cell synchronization - (reply: 1)
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Mycoplasma detection - (reply: 3)
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Can transcriptional regulation research be done in vivo? - No cell line is availbale (reply: 7)
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making competent SURE cells - (reply: 2)
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cell line rna extraction - (reply: 1)
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Cell lysis - (reply: 5)
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Suspension cells - (reply: 4)
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cell growth and colorimetric assay - (reply: 1)
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How to maintain pH of designed culture media? - Autoclaving, metabolism of cells cause changes in pH (reply: 2)
-
Another statistics data analysis Q - How to evaluate colony formation from sorted CHO cell (reply: 1)
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NK92 cell line - (reply: 2)
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EDTA to weaken cell attachement - (reply: 1)
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ccdB competent cells - (reply: 2)
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No more apoptosis, no less proliferation but less cells... - (reply: 4)
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cell line - (reply: 2)
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why my BSA contained medium cannot nourish my cells - (reply: 13)
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Trying to lyse cells to get PCR template - Need something simpler than a miniprep (reply: 10)
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cell culture - FBS (reply: 1)
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how to count the rounded cells in the well? - (reply: 4)
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Cell culture contamination, antibiotic recommendation, any recommd on Normocin - (reply: 7)
-
Problem transfecting large plasmid into HEK293 cells - (reply: 8)
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T cell proliferation/activation markers - CD69, CD38? (reply: 1)
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cell loss upon centrifugation - (reply: 5)
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Crystal violet and State's cell - (reply: 4)
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How to tell my cells are contaminated? - (reply: 3)
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Help needed! - HUVEC - C cells - RGD targeting using Human Umbilical Endothelial Cell Line - C (reply: 2)
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gene expression study - between treated and untreated cells (reply: 2)
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Inconsistency of the cells~~ - (reply: 4)
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Quantifying HeLa cells in a 96 well plate - (reply: 2)
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Epithelium cell purification - (reply: 2)
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Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
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Stable cell line stops growing :( - (reply: 6)
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siRNA protocol in floating cells - (reply: 1)
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Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
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Basics of Cell Culture - (reply: 2)
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if you plate 40 ul of your electrocomp cells only... - would you get any colonies? (reply: 13)
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Explant culture - All method of cell isolation coming from animal tissue (reply: 3)
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E.Coli. XA90 cell - transformation in XA90 cell (reply: 3)
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cells - (reply: 2)
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protocol for purificaiton of human mononucleus cells - (reply: 2)
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How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
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cell membrane - (reply: 6)
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Endothelial cells purification - (reply: 2)
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How to make mES cells attch to differentiate? - (reply: 1)
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linear or supercoiled plasmid for creating stable cell line - (reply: 1)
-
why some microorganisms form filament instead of single cell? - (reply: 1)
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can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (reply: 3)
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concentration of trypan blue... - please help me. (reply: 8)
-
how to determine the length of each phase in cell cycle - (reply: 1)
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help biochemical pathway ! - lactate effect on mammalian cells (reply: 3)
-
Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
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bEND cells proliferation assay? - (reply: 1)
-
MCF-7 cells clumped and too small - (reply: 7)
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Cells showing interesting morphology with treatment - How do I know what's happening (reply: 3)
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where to buy cell lines? - (reply: 3)
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effect of TE buffer on cells with cell lysis solution - (reply: 2)
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single channel and whole cell - (reply: 2)
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Funny looking PC12-TrkB cells - (reply: 1)
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How to break down the plant cell wall? - (reply: 1)
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Cell frozen - (reply: 1)
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transfecting adherent cells - before adherence? - (reply: 5)
-
Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
-
typical cell lysate protein concentration - (reply: 6)
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Stable cell trasfection questions... - Integration of plasmid... (reply: 5)
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Establishing cell lines from brain tumor tissue samples - (reply: 2)
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mammary stem cells - (reply: 1)
-
ICC of cell on coverslips - losing cells at permeabilization - (reply: 2)
-
A protocol needed to purify cancer cells from tumor tissue - need help! (reply: 4)
-
How to disassociate cells for FACS? - (reply: 7)
-
Myc tag and endogenous myc - Is there endogenous myc expressed in some fibreblast cells? (reply: 3)
-
How to keep cells healthy after transfection - Any tips? (reply: 5)
-
Culture of normal human mammary epithelial cells - (reply: 2)
-
cell culture - (reply: 2)
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Starving cells for stimulation - (reply: 2)
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how can I detach infected vero cells from 96 well plate without lose sample? - (reply: 2)
-
transient transfection of a stable cell line? - (reply: 4)
-
I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
-
WB with cell culture medium - (reply: 2)
-
plasmid purification from mammalian cells? - (reply: 4)
-
help- G0 synchrony of MCF10 A breast cells and MCF-7 cell lines - (reply: 1)
-
Get rid of cell debris after transformation? - (reply: 1)
-
monocytic cell line differentiation - (reply: 2)
-
Obtaining cells from human tissue samples - (reply: 2)
-
Changes on cells. - (reply: 5)
-
3T3-L1 cell differentiation - (reply: 2)
-
stable cell line questions( cell type matter) - (reply: 2)
-
Cell Culture with Human Plasma - (reply: 2)
-
cell saparation from lymph nodes and spleen - how can I improve my yield? (reply: 1)
-
G418 conc for HEK293 cells - (reply: 11)
-
Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
-
Transient transfection on a stable cell line - (reply: 2)
-
Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
-
Infection efficiency of non-adherent cells - (reply: 3)
-
Are there TGFB receptors in 293T cells? - ..and if you don't know, how would you find out? (reply: 2)
-
Split cells after siRNA transfection? - What to do when cells become confluent after transfection of siRNA (reply: 6)
-
PC 12 cell line - (reply: 4)
-
detachment of cells - (reply: 5)
-
how to identify cancer stem cell from sp cell?what is its characteristic? - cancer stem cell (reply: 2)
-
prevent cell attachment - (reply: 2)
-
Cell Banking - cell splittting and banking (reply: 1)
-
can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
-
Help: Tubulin expression is not the same between my cell clones? - (reply: 1)
-
Overlay cell culture medium with oil against evaporation - which oil? (reply: 2)
-
Why EDTA in trypsin? - (reply: 28)
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Tumour cells grow slower than normal cells, why? - cell proliferation rate in normal vs tumour cell (reply: 2)
-
paraformaldehyde solution - for cell fixation (reply: 19)
-
How to seed cells evenly in small well plates - (reply: 27)
-
remedy against cell contamination - (reply: 4)
-
stain similar to neutral red - stain unfixed cells directly (reply: 4)
-
How to lift attached CD14 cells? - (reply: 3)
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How can i decontaminated my cell culture ? - (reply: 4)
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Cell fixation problem - (reply: 3)
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Hela cells and stable transfection help please! - (reply: 6)
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Is cell culture considered in vivo? - (reply: 4)
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Transfecting KG 1 cell - (reply: 3)
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cell stain, nonfluroescent - (reply: 4)
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Need to Slow down Cell Culture growth - (reply: 2)
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What size needles I should use for cell extraction protein - (reply: 1)
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Cell adhesion assay - (reply: 2)
-
Tetracycline in cell culture media - (reply: 5)
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Treatment of cancer cell - (reply: 2)
-
why centrifuge before doing a cell count? - (reply: 1)
-
amount of proteins isolated from cells - (reply: 2)
-
clumping cells - s. aureus wildtyp cells (reply: 3)
-
Primary Hippocampal cell culture-DEAD! - (reply: 3)
-
How long can cells survive at 4 degree - (reply: 2)
-
Medium for adherent cells and serum/protein free - (reply: 2)
-
Mouse ES cell won't attach - (reply: 1)
-
monitor cell growth - (reply: 1)
-
RNA extraction from formaldehide fixed cells - (reply: 2)
-
vector for expression of tag on cell surface - (reply: 7)
-
Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
-
why competent cells take up only 1 type of plasmids? - (reply: 7)
-
cell lysis buffer recipe - simple question (reply: 6)
-
Help with HeLa cells culture... - (reply: 10)
-
FACSing of MCF-7 cells - (reply: 3)
-
Transfecting primary cells (imr90) - PROBLEM - (reply: 4)
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Why my cells dont grow - hippocampal cell (reply: 5)
-
PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
-
treat cells with progesterone and estrogen - (reply: 1)
-
difference between treating cells with ATP disodium salt and ATP magnesium salt - (reply: 2)
-
cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
-
growth rate of SK-BR-3 cells - (reply: 3)
-
neural stem cell markers? - (reply: 2)
-
G418-selected cells do not divide - (reply: 8)
-
unwanted cell clumping after splitting MCF7 cells - (reply: 5)
-
hematopoietic stem cell trans-differentiation - (reply: 4)
-
T cell culture - (reply: 2)
-
differentiating live from dead bacterial cells - (reply: 2)
-
Long-time storage of tissues/cells with fluorescent tracers - (reply: 4)
-
Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
-
Why is non-viral transfection efficiency of endothelial cells low? - (reply: 1)
-
Hepg2 cells resistant to trypsin digestion - (reply: 7)
-
Immunofluorescence of frozen cell culture inserts - (reply: 6)
-
T cell proliferation assay - (reply: 1)
-
Stem Cell growth on soft agar - (reply: 3)
-
Cell transfection - TransIT vs Fugen - (reply: 3)
-
Debris or particles in medium - A549 cells and weird particles (reply: 4)
-
far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
-
Cell surface antigens - identification (reply: 8)
-
How long can the whole cell extract for ChIP stay in -80C? - (reply: 1)
-
Overexpression of Viral Protein in mammalian cell line - (reply: 3)
-
Chemotaxis Assay for Primary Mouse Peritoneal Cells - (reply: 7)
-
Getting rid of the dead cells during selection of transfected colonies? - (reply: 2)
-
optimizing selection of transfected cells - (reply: 2)
-
MTS assay of MCF7 and MDA-MB-453 cells - (reply: 1)
-
primary cell culture death - (reply: 8)
-
ELISA IL12 p70 from mouse dendritic cells culture SN - (reply: 2)
-
new lab, cells die after passaging - general fish cell culture methods (reply: 5)
-
Fibroblast cell line - (reply: 2)
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FBS or FCS for NIH3T3 cells - How to grow these cells optimal (reply: 7)
-
Cell density to platte after trypsinization - (reply: 3)
-
3T3 cells and lysis buffer recepie - western blot (reply: 1)
-
Starvation of cells for higher uptake? - (reply: 2)
-
mouse spleen cell culture - (reply: 2)
-
a question about splitting MCF-7 cells - (reply: 5)
-
Trypan blue cell death assay on cardiac myocytes - (reply: 3)
-
What is an acceptable CO2% range for growing cells in DMEM? - (reply: 8)
-
mycoplasma effects on cells - will mycoplasma kill the cells? (reply: 2)
-
method for staining of invisible cell pellets? - (reply: 1)
-
rna isolation from bone marrow cells - (reply: 1)
-
Monocyte-derived DC generation: CD14 separation or adherent cells better? - (reply: 1)
-
GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
-
transplantable rats or mice cell line in nude mice - (reply: 1)
-
How to tell cell are contaminated by fungi - (reply: 4)
-
Need protocol: CFSE Staining/ primary cells - (reply: 2)
-
why do you prepare heat-stable fractions of cells? - (reply: 1)
-
Puromycin and HEK293 cells - (reply: 3)
-
neuronal markers for the mature level for spinal cord cell - (reply: 4)
-
tissue n cell culture - (reply: 3)
-
do cells benefit from being of different age? - (reply: 2)
-
cell culture contamination - (reply: 2)
-
Can I call my cell a established cell line? - (reply: 1)
-
how long can cell lines be carried? - (reply: 5)
-
Could we freeze the 0.25 trypsin EDTA and thaw it, use it? - (reply: 8)
-
transfection of HeLa cells - (reply: 4)
-
cell stain and the fixation? - (reply: 2)
-
Cell Differentiation - Time periods and environment for proper cell differentiation (reply: 1)
-
Cell Lysis - (reply: 2)
-
Does anybody have MIN6 cells? - MIN6 Cells (reply: 1)
-
SiRNA and GFP transfection MCF7 cells - (reply: 4)
-
questions about MCF-7 cells - (reply: 10)
-
detachment of my cells?/ - (reply: 7)
-
depolymerizing microtubules in 293 cells - (reply: 1)
-
cell proliferation assays - (reply: 2)
-
Retroviral transduction of cells - troubleshooting help please! - (reply: 8)
-
After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
-
should dead cell detach? - (reply: 2)
-
how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
-
Stem Leydig cells - (reply: 1)
-
adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity (reply: 3)
-
problems with Clonetech 10G cells - (reply: 1)
-
THP-1 cell line - Passage number affect phagocytosis (reply: 12)
-
MTT test for the suspension culture - Raji cell and K562 (reply: 4)
-
maximum plasmids can be co-transfected into Hela cells - (reply: 4)
-
T cell proliferation assay - (reply: 3)
-
Culture of adherent SH-Sy5y Neuroblastoma Cell Line - (reply: 5)
-
CCR5 cytometry on dendritic cells, probles with isotype control - (reply: 2)
-
lymphocytes and hepatocytes are the same!? - (reply: 4)
-
transfection of MCF7 cells - (reply: 2)
-
Has anyone transfected ST15A cells? - Transfection of ST15A cells? (reply: 3)
-
cell harvest by scraping versus trypsinization - (reply: 21)
-
ethanol/IPA kill cell - (reply: 1)
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Cell culture Contamination - Again! (reply: 13)
-
PBS and Cells - How long can cells sit in PBS? (reply: 6)
-
Freeze/Thaw procedure in cell lysis - How is this done??? (reply: 12)
-
cell death during transfection - (reply: 3)
-
Best way to detect shRNA produced in cells? - (reply: 2)
-
flow cytometry - labelling surface receptor on monolayer cells (reply: 5)
-
PMA activation of cells - (reply: 5)
-
losing green light form pIRES2-EGFP transfected cells - (reply: 13)
-
Help, Neuroscience cell lines - (reply: 1)
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How to trace siRNA in cells - (reply: 4)
-
stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
-
Impact of dead cells in a culture? - When cells die, how toxic can they become to viable cells? (reply: 4)
-
Removing adherent cells for Flow cytometry without trypsin - how to do it (reply: 3)
-
Glioblastoma cell lines and transfection - stable transfection (reply: 5)
-
Problem establishing a stable PC12 cell line - (reply: 6)
-
How to make 3T3 mouse fibroblast cell bank? - (reply: 1)
-
how to know that ur cells are confluent? - (reply: 2)
-
How to make a stable cell line? Help! - (reply: 3)
-
time when cells go into apoptosis - (reply: 2)
-
doxorubicin treated cell cutlures? - (reply: 1)
-
Cell line passage number? - (reply: 3)
-
basic techniques for cell treatment - (reply: 6)
-
Trypsin-Free Detachment of cells using EDTA - (reply: 10)
-
The mechanism of the cell "fix" reagent? - (reply: 1)
-
adding antibiotics to cho cell media - (reply: 2)
-
Can the monocytic cell line MD (ATTC no. CRL-9850) be differentiated into dendri - (reply: 1)
-
ELISA with fixed bacterial cells - (reply: 1)
-
cells not adhering to flask after thawing - (reply: 14)
-
obtaining cell clone? - (reply: 3)
-
establishment of stable cell line - (reply: 2)
-
cell cetrifugation_how fast can you go? - (reply: 6)
-
What's the maximum amount of DMSO you can use in cell culture? - (reply: 4)
-
how to avoid MEF when mES cells are transfected by lentivirus? - (reply: 4)
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TB1 Competenet Cell - (reply: 2)
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293 cell lines - (reply: 4)
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Growing PC12 cells on glass - (reply: 3)
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how to set up a stable cell line? - why false positive always appears? (reply: 2)
-
RNA extraction from cells grown in matrigel - (reply: 1)
-
Is there a quicker way of making stable cell line? - (reply: 7)
-
How to deplete calcium from cell medium? - (reply: 1)
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Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
-
cell separation by centrifugation - (reply: 1)
-
cell line nonresponsiveness - is it a time to change my cell line???? (reply: 2)
-
Cell cycle progression and arrest - (reply: 1)
-
Cheap and quick cell viability assay - (reply: 11)
-
siRNA transfection of MIN6 cells - (reply: 2)
-
Is it possible to harvest E.coli cells for purification of DNA from LB agar and - (reply: 5)
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Cells survived after G418 selection are transfected cells? - (reply: 2)
-
Saos-2 cells transfection - (reply: 1)
-
how to make neutrophil cell lysates for Western blot? - (reply: 3)
-
getting rid of shaky hands during cell culture/pipetting - (reply: 9)
-
Mouse endothelial cell line - (reply: 1)
-
trypsin peptide extraction storage - (reply: 3)
-
infected cell line? - fungal contamination? (reply: 5)
-
Growing cell lines from frozen tissue samples - Is this even possible??? (reply: 8)
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Cell Growth - Help! (reply: 7)
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Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
-
Trypsin:EDTA cell detachment process - (reply: 6)
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Trypan Blue exclusion - (reply: 2)
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Cell lysing for western blot (Help!) - (reply: 2)
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retroviral transfection of pheonix cells - (reply: 3)
-
how to build a stable transfection in a suspension cell - (reply: 1)
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How to culture stable cell lines? - (reply: 3)
-
effect of enzymes on cell membrane - (reply: 4)
-
confocal assay for FITC-labled peptide in Hela cell - (reply: 1)
-
Primary culture hepatocytes of mouse - (reply: 2)
-
Techniques for counting apoptotic cells - (reply: 6)
-
Persistent cell culture contamination by fungi and bacteria - Cell culture, cell biology (reply: 6)
-
Adult stem cell differentiation - (reply: 2)
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Cell Lysates for western - (reply: 4)
-
transfecting DNA into Jurkat cell - (reply: 1)
-
single cells from human tissue? - need protocols (reply: 1)
-
Tryphan Blue staining for cell viability - (reply: 1)
-
Anyone has experience to grow sespension 293 cells? - (reply: 1)
-
Cornea Epithelial Cell culture - (reply: 1)
-
what kind of adherent cell lines can be analyzed by FCM through Annexin V/PI sta - (reply: 2)
-
cell cycle data normalization - (reply: 1)
-
Why are lysosomes considered the cell's "cleanup crew"? - i need help (reply: 1)
-
Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
-
Can I extract nuclear proteins in transfected cells? - (reply: 2)
-
Ordering CHO-K1 cells - (reply: 1)
-
why my IFN gamma treated cells still high in TER? - (reply: 1)
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co-transfection in NRK cells - (reply: 1)
-
293T cell line, how to prevent cell clusters? - (reply: 1)
-
Cell cycle analysis - Include floating cells or not?? (reply: 7)
-
Death of Cells After Defrosting - MDBK cells not surviving (reply: 2)
-
Extensive cell death after transfection in a 24-well plate - (reply: 3)
-
Hela cells clumping in center of well of 96well plate. - (reply: 1)
-
mouse B cells - (reply: 1)
-
reasons for apoptosis in primary cell culture? - (reply: 2)
-
Problems with cell lysate - (reply: 4)
-
competent cells - making compentent cells (reply: 2)
-
Establishment of human embryonic stem cell - (reply: 3)
-
Help: got the stable cell line without my target gene - (reply: 3)
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Sticky cells - (reply: 3)
-
ask for 721.221 cell line - (reply: 3)
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Spectrophotomery of cell 550 vs. 600 - spectrophotometry for cells (reply: 2)
-
extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
-
looking for a good siRNA transfection reagent for 3T3 L1 cells ? - (reply: 5)
-
endothelial cells transfection - (reply: 2)
-
How many passages cells lines can survive before senescence? - (reply: 8)
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RNA extraction from small number of cells - (reply: 3)
-
NMDA tox and Rat Cerebellar Granular Cells ! - NMDA tox assay for Rat CGC's ???? (reply: 3)
-
Host cells for pBELOBAC11 - (reply: 4)
-
URGENT-Selecting for transformed bacteria cells - (reply: 1)
-
mammalian cells invaded by intracellular microbes - how can I decontaminate? (reply: 3)
-
RAW 264.7 cell line transfection - (reply: 1)
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change adherent cells into suspenion cells - (reply: 1)
-
detection of GFP fluorescence in whole cell bacteria - (reply: 1)
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How to fix and stain lymphoblastoid cell onto glass slides - (reply: 5)
-
no growth on plate agar for competent cell - (reply: 6)
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starvation medium recipe for Hela cells - (reply: 2)
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Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (reply: 1)
-
transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
-
siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
-
problem transfection Hela cells - (reply: 8)
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How to study the viability of cells - (reply: 10)
-
cell lysis? - (reply: 2)
-
cell death after subculture - (reply: 3)
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can you introduce mutation directly into the cells? - other than just transfecting it with a vector... (reply: 1)
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Mystery - Cell Proliferation Assay - (reply: 6)
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Help with Cell attachment problem - (reply: 3)
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How many cells to lyse for Western? - Decreasing signal in my Western Blot (reply: 2)
-
Human Neural Stem Cells wanted - (reply: 1)
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plating cells for transfection - (reply: 2)
-
cytotoxicity and cell proliferation assay - preference of dyes for cell viability (reply: 2)
-
protocol for electrocompetent cells preparation - (reply: 5)
-
Transient transfection on a stable cell line - (reply: 6)
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especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
-
need help for culture protacal of new stem cell - (reply: 5)
-
Activating human T cells - (reply: 8)
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Storage of whole cell lysates - How? (reply: 2)
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Why use PBS before trypsinizing cells - (reply: 4)
-
Question about doing RNAi in primary cells - (reply: 3)
-
A Procedure for Freezing and Thawing Bacterial Cells - (reply: 2)
-
Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
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Cell Tracing Software - Searching for a quicker way!!! (reply: 2)
-
cells going away - (reply: 3)
-
pituitary cell lines sex - (reply: 1)
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Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (reply: 4)
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How to tell if competent cells or SOC are contaminated? - (reply: 14)
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Transcient transfection of HaCaT cells - which is the most effective method? - (reply: 2)
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Dissociation of HaCaT cells without using trypsin - will PBS/EDTA work? - (reply: 4)
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Where to get Granta 536 Cells - (reply: 4)
-
Moving Dots in cell culture - Contamination? - (reply: 7)
-
Drosophila cell culture - (reply: 3)
-
Culture problems of CRL-2331 cells (ATCC) - (reply: 3)
-
electroporation of PC12 cells - (reply: 1)
-
RNA prep (Trizol) with small cell numbers - How low can you go? (reply: 13)
-
Slow growing cells - (reply: 6)
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About cell diameter - Help,please browse (reply: 1)
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Protoreaction of Protoplasm - New approaches to Cell Biology and Physiology (reply: 1)
-
Problems with Ba/F3 cell culture - (reply: 1)
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Washing with Trypsin EDTA - (reply: 2)
-
CHO Cell revival - (reply: 2)
-
How to isolate and culture murine epidermal langerhans cells - (reply: 2)
-
Neural Marker For Cell Typing and Maturation - Neural Marker For Cell Typing and Maturation (reply: 4)
-
Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy (reply: 3)
-
Stem cell transfection - (reply: 2)
-
cell detachment - help (reply: 3)
-
Able C and Able K competent cels - Has anyone use these cells before (reply: 2)
-
bioinformatics in cell signalling - use of bioinformatics (reply: 1)
-
Marker for plasma cell staining - (reply: 1)
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AGS Cell - Medium for AGS Cells (reply: 2)
-
how to isolate T cell and DC in the same sample? - (reply: 2)
-
Minipreps from Genehogs cell line - (reply: 1)
-
Sick primary cells - (reply: 1)
-
Does anyone know some specific marker of mouse hepatocytes? - (reply: 2)
-
How to culture rat dorsal root ganglia cell? - (reply: 1)
-
Protocal for DAPI/ Hoechst staining adhering cells - (reply: 3)
-
how 2 quantify bacteria cells? - (reply: 4)
-
Shipping cells in suspension culture - (reply: 6)
-
hela cells - (reply: 12)
-
cre recombinase - in cell lines (reply: 2)
-
siRNA transfection reagent for primary cultured cells - (reply: 2)
-
pcDNA 3.1/pTarget and stable cell line - (reply: 10)
-
Cells are stuck - (reply: 4)
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Serum for cell culture - difference on regular vs dialyzed vs heat-inactivated FBS (reply: 3)
-
Red Blood Cells exploding ! - (reply: 2)
-
How does the cells of the innate immunity gets rid of the pathogens? - (reply: 4)
-
Baculovirus Expression - Sonicate infected cells? (reply: 2)
-
Synchronize hela cells with double thymidine block - mechanism of double thymidine block (reply: 1)
-
house-keeping primers for Cos7 cells - (reply: 4)
-
RAW264.7 Cells .. anyone familiar with this cell line? - need help please (reply: 2)
-
adapting cells to CO2-Independent Medium - (reply: 1)
-
DNA methylation in Raji cell line - (reply: 1)
-
How long does take the cell cycle in HepG2 cell line? - (reply: 1)
-
T cell activation. - (reply: 1)
-
Alizarin red/Trypan blue stain - (reply: 3)
-
coat culture dish for HEK-293T cells? - (reply: 5)
-
problems with Ghost cells - (reply: 1)
-
bisulfite sequencing: cell lines vs tumors - (reply: 3)
-
cell type specificity ? - (reply: 1)
-
Passage number and behaviour of cell line - (reply: 10)
-
Mycoplasma Testing in culture medium - (reply: 2)
-
Anti Lamp-1 or Lamp-2 for COS cells? - (reply: 3)
-
Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
-
will the passage number affect the performance of THP-1 cells - (reply: 1)
-
A drug to inactivate glial cells - (reply: 2)
-
Duration of cell line transfected - (reply: 2)
-
antibiotic dose response for suspension cell - (reply: 2)
-
Emergent: Toxicity of cell lysis buffer :( - (reply: 2)
-
Help with endothelial cells - Endothelial Cells Culture (reply: 4)
-
Cell lysis for westerns? - Immediately after harvest?? (reply: 8)
-
Quantity of genomic dna in human cells - (reply: 5)
-
siRNA Stable transfection in human fibroblast cells - (reply: 6)
-
Cell proliferation/viability assay - use of WST and 1-Methoxy PMS (reply: 4)
-
Add L-glutamine in cell culture medium? - (reply: 5)
-
Mycoplasma contamination - detection and de-contamination (reply: 7)
-
transfection of Hek 293A cells - (reply: 5)
-
cell scraper - (reply: 4)
-
Trypsin does not work - need to detach monocytes (reply: 12)
-
Transfection of cells by viral DNA - (reply: 4)
-
neural progenitor cell death in multi-well plate - (reply: 10)
-
Hybridoma Cell lines - How to do it (reply: 1)
-
more contamination owes - spherical cell like contamination (reply: 8)
-
C2C12 cells model for differentiation - (reply: 1)
-
about glucose uptake test in hepatocytes - (reply: 2)
-
Glycerol gradients - glycerol gradients for cell extract fractionation (reply: 2)
-
Fibroblast and epithelial cells in tumor samples and primary cultures - (reply: 2)
-
DNA Repair in different cells - (reply: 2)
-
Getting crazy because of cell contamination - (reply: 27)
-
lncap cell culture - (reply: 3)
-
cell fractionation plus IP - (reply: 12)
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Lab dealing with factors affecting cell membrane permeability - (reply: 1)
-
passaging 293T cells - (reply: 5)
-
lysosomes in cells - (reply: 2)
-
Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
-
making more chemically competent cells - (reply: 6)
-
Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
-
anyone has EcR293 cells? - (reply: 5)
-
Searching specific Cell line - (reply: 1)
-
Help me....About the 3T3-L1 Cells - (reply: 2)
-
Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
-
HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
-
How many cells (nuclei) per ml pellet? - (reply: 3)
-
Glycyrrhizin (GL) and cell culture - (reply: 1)
-
transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
-
ES and MEF cell - (reply: 1)
-
How to reduce cell clumping for immunocytostaining - (reply: 1)
-
primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
-
ATCC cell lines - How is your experience? (reply: 1)
-
siRNA transfection of non-adherent lymphoma cells - (reply: 2)
-
need help on learning cell and tissue culturing - (reply: 6)
-
can I re-freeze the 4% paraformaldehyde for longer storage? - leave cells in 4% paraformaldehyde 4 FACS? (reply: 3)
-
Genomic DNA purification from few Tissue Cultured cells - (reply: 2)
-
PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
-
How to grow adherent cells without attachment??? - (reply: 2)
-
mouse Fibroblast specific surface markers??? - how to gate out mouse Fibroblast cells in FACS (reply: 1)
-
what's the best system to get siRNA into a cell? suggestions - (reply: 1)
-
Lysis buffer - muscle cells lysis (reply: 1)
-
Help with defective cell lines - (reply: 1)
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How to measure neuronal cell differentiation? - (reply: 1)
-
Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
-
Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
-
Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
-
Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
-
Trypsin vs Non Enzymatic - (reply: 2)
-
transfection - selection of trransfected SH-SY5Y cells (reply: 3)
-
Who killed my cells??? - Toxicity during transfection (reply: 3)
-
Transfection reagent for HT-29 and HCT-116 cells - (reply: 4)
-
MCF-10A transfection question - what to do with slow growing cells. . . (reply: 1)
-
FACS - permeabilization of cells without killing (reply: 1)
-
DC harvesting for FACS - dendritic cells (reply: 2)
-
about cell sonication - (reply: 2)
-
Subculturing cells grown in serum free media - (reply: 2)
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How to screen mammalian cells clones? - (reply: 3)
-
Commonly used endothelial cells - (reply: 2)
-
aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
-
making homogeneous cell lysate suspensions - (reply: 1)
-
How to injure cell wall of chlorella for transformation - (reply: 2)
-
transfection of differentiated Caco-2 cells - (reply: 1)
-
Cell cycle (phase) analysis by FACS analysis - PI staining to gate different phases of cell cycle (reply: 1)
-
Mycoplasma - input needed! - Please help me by answering these questions! (reply: 6)
-
Cell attachment - cells and jam (reply: 1)
-
Immunofluorescence - cell surface protein detection - How do I detect only cell surface expressed prot. (reply: 3)
-
How to choose the flask shape and volume for Vero cells? - Is it possible to reduce? (high-cost reagents) (reply: 1)
-
cell lines and their particularities - where is the info (reply: 1)
-
Separation of dead cells from cultures - (reply: 8)
-
methods to 'quantify' morphology change in siRNA treated cells - (reply: 1)
-
How to avoid contamination of primary cells during preparation - (reply: 1)
-
RNAi in CHO cells - (reply: 5)
-
How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
-
Mold Contamination in cell culture - Mold and Cell Culture (reply: 7)
-
competent cells question - (reply: 3)
-
Growing cells on soft agar - MCF-7 cells on agar plates (reply: 1)
-
What the chance to get live cells? - (reply: 11)
-
Transfection of 293T cells on coverslips - (reply: 1)
-
Clumping of MCF7 cells - (reply: 3)
-
Cell specific Sonication - How different are cell types (reply: 1)
-
Cell counting - (reply: 2)
-
Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
-
Sonicating cell lysate for Westerns - (reply: 2)
-
has anybody done live cell staining? - (reply: 6)
-
Putting 2 plasmids in 1 e. coli cell - (reply: 4)
-
inducible stable cell line.... - (reply: 1)
-
assays to assess cancer cell growth? - (reply: 4)
-
Transforming DH5 alpha cells with pUC19 vector - Cloning (reply: 3)
-
Insect cell sonication protocol? - Protein Purification (reply: 1)
-
Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
-
best cell colture media for in vivo imaging - (reply: 3)
-
A question about cell stain: fix the cell have fusion protein - (reply: 1)
-
How to get rid of contamination in stable transfected cells - (reply: 3)
-
how can i culture virus in primary cell lines? - microbiology (reply: 1)
-
coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
-
can i freeze cell lysates for western blotting? - (reply: 3)
-
real-time RT-PCR one cell - (reply: 2)
-
Subculturing astrocyte from E15 days mouse embryo - Technique (reply: 2)
-
Lipof/Opti transfection cells died in DMEM/F12 - Transfection (reply: 4)
-
Why add phenol red in trypsin? - (reply: 14)
-
Cell cycle question - CKIs - (reply: 2)
-
Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
-
Definition of cell confluency - (reply: 6)
-
How to seed adhering cells (HepG2) evenly in 24-well plates? - (reply: 5)
-
Incubation - Effect of other cell lines during incubation (reply: 1)
-
growing epithelial cells from tumor - (reply: 2)
-
In Vivo cell proliferation - (reply: 3)
-
detecting Mycoplasma in cultures - (reply: 7)
-
Do you reuse cell culture vessels? - (reply: 3)
-
more help: cell culture problem - (reply: 1)
-
Isolation of mouse hepatocytes using Percoll - Protocol for isolation of mouse hepatocytes (reply: 3)
-
get metaphase cell by nocodazole? - (reply: 1)
-
Passage-hepatic stem cells - (reply: 1)
-
Establishment of primary cell lines - (reply: 1)
-
Fungal Infection in mesangial cells - Primary Cell Culture (reply: 2)
-
Freezing medium and cell counting - (reply: 6)
-
Tumor cells immortal? - (reply: 5)
-
"Cell culture discard in chloros (10,000) ppm" ? - (reply: 4)
-
cells transfection - transfecting DNA amounts (reply: 5)
-
human rTGFß for mouse cell culture applications? - (reply: 1)
-
Gene expression in mammalian cells - (reply: 6)
-
Thawing of competent bacterial cells - (reply: 2)
-
Origins of primate, canine, vertebrate cells, etc. - (reply: 1)
-
Tissue culture and cell culture? - (reply: 4)
-
Do all 293 cells contain an adenovirus? - Do all 293 cells contain an adenovirus? (reply: 3)
-
seeding of cells for transfection - (reply: 4)
-
Liver Perfusion - Hamsters Liver Perfusion and Hepatocytes isolation (reply: 2)
-
how to prevent HEK293 cells from coming off the plate - (reply: 4)
-
transfection problem---SOS! - why the cells should be split before transfection? (reply: 7)
-
Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
-
MDA-MB-231 cell culture - (reply: 7)
-
Preparation of competent cells of pichia - (reply: 1)
-
Problem in culturing PZ-HPV-7 cell line - (reply: 4)
-
Murine markers on hematopoietic stem cells (CD34, Sca-1, etc) - Looking to identify markers on megakaryocytic progenitors (reply: 2)
-
breast primary culture cells - (reply: 2)
-
filtering for cell culture stuff - filter cell culture (reply: 5)
-
transfection of single copy of plasmid per cell - (reply: 3)
-
looking for cell line: CEMX174(T1) - (reply: 2)
-
SF9 cells - (reply: 2)
-
Natural Killer cells - (reply: 1)
-
Stable Transfection in Sf9 cells - Stable Transfection in Sf9 cells (reply: 5)
-
B-gal staining of cells - (reply: 1)
-
Need HeLa cell extract prepartion protocol - wait your answer online (reply: 1)
-
bands from tissue one size, from cells - size doubles - help! (reply: 4)
-
Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
-
red blood cell lysis - (reply: 6)
-
RNAi in Jurkat cells - (reply: 3)
-
Transfection - Cell death after transfection - (reply: 6)
-
FACS analysers - Purchase of a FACS analyser and cell sorter (reply: 3)
-
Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
-
co-culture of two adherent cells - (reply: 4)
-
Cell lysis when isolating dendritic cells - (reply: 1)
-
hep-2 & Hep-G2 cell line - difference between hep-2 & Hep-G2 cell line (reply: 22)
-
Cell contamination, needs the help urgently - How to remove? (reply: 2)
-
EDTA only for cell dissociation - (reply: 7)
-
where to buy sterile grease used for isolate cell clone? - (reply: 2)
-
please recommend me a RNA isolation kit - RNA isolation from 100 tumor cells (reply: 1)
-
Anybody has ever transfected EL4 cells with siRNA? - help! (reply: 3)
-
RT-PCR of different cell lines - (reply: 1)
-
cancer cell culture - protocols on cancer cell culture (reply: 8)
-
Mammalian cell line - monolayer - monolayer growth of mammalian cell line (reply: 1)
-
How many cell passages to be safe ? - (reply: 8)
-
Contamination issue in cell culture - (reply: 109)
-
Visualizing Cell Pellet - (reply: 1)
-
LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
-
Stable transfection of S2 cells - (reply: 1)
-
ATDC5 cell line - i am looking for this cell line (reply: 2)
-
Dendritic cell -isolation ---viabillity is very low - Immunology (reply: 3)
-
measuring apoptosis after treatment of cells with ZVAD-FMK - (reply: 1)
-
Suspension Culture - Can it be transformed into adherent cells? (reply: 5)
-
Help! Cell staining problem - (reply: 3)
-
choosing insect cell for high-cystine protein expression - (reply: 1)
-
Cells in collagen gel - Growing mammalian cells in collagen gel (reply: 1)
-
H295R Cell transfections - (reply: 2)
-
cells in the earth - (reply: 4)
-
Trypsin free detachment?? - (reply: 2)
-
Freezing and thawing of cells - (reply: 112)
-
Human Macrophage cell line - quick help please - (reply: 3)
-
cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
-
Primary epithelial cell line - Isolation of epithelial cells (reply: 3)
-
CaPO4 transfection prevents trypsin activity - Trying to split after transfecting (reply: 1)
-
single cell PCR - (reply: 8)
-
Transfection using PEI - Transfection of 16HBE and CFBE cells (reply: 4)
-
Tet-responsive cell line - cells cannot become confluent (reply: 1)
-
293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
-
What are the cells comprising PBMC?? - (reply: 1)
-
MC57G cells and transfection - (reply: 1)
-
transfection efficiency - mammalian cell transfections (reply: 1)
-
pBABE Comp. Cells - Special cells for transformation (reply: 3)
-
Any ideals for cell fragmentation? - (reply: 2)
-
cell lysis - How to lysis cells (reply: 2)
-
DMSO and cell cycle - (reply: 2)
-
cell culture RNA extraction - cell culture RNA extraction (reply: 4)
-
Origami B cells grow too slowly!! - (reply: 1)
-
about LNCap cell lines - (reply: 1)
-
CBP expression problems in mammalian cells - (reply: 1)
-
problem by using percoll to purify primary cell - (reply: 1)
-
the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
-
T cell proliferation - problems (reply: 1)
-
lymphocytes' RNA extraction - How many cells (reply: 1)
-
Selection of cell ine - (reply: 2)
-
Cell culture problem - weird stuff grow in my cells - (reply: 6)
-
Cell counting of Strongly adherent cells - (reply: 4)
-
How to detect drug's cytotoxcity by MTS - (reply: 2)
-
How to assay the amount ofcollagen on cell/scaffold composites? - (reply: 1)
-
PBMC-B cell - (reply: 1)
-
Plant Culture Cell - (reply: 1)
-
stable cell line - stable cell line (reply: 1)
-
Cell line for glutamate receptors - Glutamate receptor cell lines (reply: 1)
-
lipofectin mediated transfectionof HepG2 cells - (reply: 1)
-
stable transfection of epithelial cells - stable transfection in mammary breast cell lines (reply: 8)
-
Shipping Cell Cultures - Shipping Cell Cultures (reply: 3)
-
using culture sup of one cell line to induce 2nd cell line - (reply: 1)
-
Stable Trasfection into Hek 293and Hela cells - (reply: 1)
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Isolation of nuclei from mammalian cells - (reply: 1)
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Re: how to seperate deadsuspension cells from alive ones - (reply: 1)
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mutation in cancer cell line - (reply: 1)
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Reviving Cells from Liquid Nitrogen - (reply: 2)
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cell lines for anti-inflammatory study - (reply: 1)
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How to tell different cellstypes apart - (reply: 2)
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Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
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Centrifuge cell at room temperature? - (reply: 1)
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cells washed away - cell culture problems (reply: 6)
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cell viability/toxicity - (reply: 3)
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Stable Transfection of CEM cells - (reply: 1)
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seperation of epithelial cells and fibroblast from a biopsie - a culture of epithelial cells is needed without fibroblasts (reply: 1)
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total protein assay in cell extracts - (reply: 3)
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Labeling cells with AT32P - cell biology (reply: 1)
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how the seperate dead cells from living ones. - (reply: 5)
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animal cell culture - (reply: 2)
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distilled water shock of red blood cells - (reply: 4)
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please help me - how to extract cd3 protein from jurkat cell (reply: 1)
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Influenza inoculation of Hek293 cells - (reply: 1)
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Quantifyng Mycobacterium Cells - (reply: 5)
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Growth conditions for T cellline - (reply: 2)
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Mouse B cell Activation - (reply: 1)
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Cell isolation form cord blood - (reply: 1)
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Is there any simple way totell a cell coming from cell division - (reply: 1)
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T cell isolation from rat wholeblood - (reply: 2)
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Pituitary cell culture - (reply: 1)
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Whole Cell PCR from BacteriaProtocols? - (reply: 4)
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collagen measurement andisolation in cell based - (reply: 1)
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genomic and fragmented DNAextraction from human cells. Ple - (reply: 1)
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Units of trypsin? - (reply: 1)
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RNA isolation from a single cell - (reply: 1)
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Competent Bascillus Cells - (reply: 1)
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cell lysis - How do I decide what solution to use? (reply: 2)
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adenovirus infection (293 cells)protocol - (reply: 1)
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cell line with deletion in mitochondrial DNA - Molecular Biology (reply: 1)
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Cell senescence markers - (reply: 1)