Cells die after changing medium - Is there role of FBS? (Oct/03/2008 )
I got a cell line from a friend. The cells grew well in the medium he provided. But when I changed the medium, the cells started dying. I had already used the same lot of medium and FBS for culturing the same cell line earlier without any problems until I lost my cell line due to contamination. The lot of cell line that I got from my friend did not grew in my medium.
-rpsingh-
Then probably your media is not right. Check the components in your media and verify with your friend about the media.
-scolix-