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How to evaluate Cell viability - (Nov/08/2006 )

Hello Friends,
I am performing the MTT assay to determine the cell viability. I have done the experiment in 96 well plate format, with different drug concentration treatment with positive control and without treatment control.
I got the different OD s from spectrophotometry. Could you please tell me , that how could I get I get the viablity percentage from this assay?

Thanks
Deb

-debarshir-

QUOTE (debarshir @ Nov 8 2006, 06:27 PM)
Hello Friends,
I am performing the MTT assay to determine the cell viability. I have done the experiment in 96 well plate format, with different drug concentration treatment with positive control and without treatment control.
I got the different OD s from spectrophotometry. Could you please tell me , that how could I get I get the viablity percentage from this assay?

Thanks
Deb


relative to untreated control in percent

-The Bearer-