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how to transport a culture cell 35 mm plate or 25cm2 flask - confluence is very very slow (Oct/13/2008 )

I need your advice.!!!!!!!

I’m going to travel for 14 h and I need to carry a cell line culture. Of course I couldn’t get a frozen vial in dry ice, and I have very few cells, it’s is a very difficult line to acquire, and the only cells I have is a 20-30 % confluence culture in a 35 mm plate (I have no more) and the cells duplicate very very slow. I’m traveling in Wednesday, the question is: which is better??
Let the cells in that 35 mm plate and closed with parafil. Or
Tripsinizate and culture them in a 25 cm2 flask today to be better closed, have less risck of contamination and cells have more medium. No mater confluence is going to be very low.

It is important because is the only cells I have and would be very dificcult to acquire!!!!! ohmy.gif

-aztecan princess-

QUOTE (aztecan princess @ Oct 13 2008, 10:04 AM)
I need your advice.!!!!!!!

I’m going to travel for 14 h and I need to carry a cell line culture. Of course I couldn’t get a frozen vial in dry ice, and I have very few cells, it’s is a very difficult line to acquire, and the only cells I have is a 20-30 % confluence culture in a 35 mm plate (I have no more) and the cells duplicate very very slow. I’m traveling in Wednesday, the question is: which is better??
Let the cells in that 35 mm plate and closed with parafil. Or
Tripsinizate and culture them in a 25 cm2 flask today to be better closed, have less risck of contamination and cells have more medium. No mater confluence is going to be very low.

It is important because is the only cells I have and would be very dificcult to acquire!!!!! ohmy.gif



Grow your cells in T25, fill it up with medium, wrap it around with papafilm, you are ready to go.

-genehunter-1-

Thanks!!!! That’s what I was thinking but I wasn’t sure!! I needed other opinion. smile.gif

-aztecan princess-

QUOTE (genehunter-1 @ Oct 13 2008, 09:32 AM)
Grow your cells in T25, fill it up with medium, wrap it around with papafilm, you are ready to go.


I agree.

less chance of spilling the medium compare to plate

-Minnie Mouse-