androgen solution for cell treatment - (Sep/23/2007 )

Hi All,
I want to assay the effect of androstenedione and testosterone on cultured cells. I don't know what solvent to use. can anyone help?

Hi,

we use R1881 in absolute EtOH.

Cheers

Hi zek,
what is R1881 and isn't absolute EtOH harmful for cells?
thank you

the ethanol should get diluted sufficiently when introduced to the cell culture medium.

thank you very much for your reply.

Hi,

sorry for delayed answer. R1881 is the synthetic androgen hormone. We use 10exp-8 final concentration in treatments. The working stock is 10exp-5. So one adds 10ul of the working stock to 10ml medium.

Cheers

When we prepared our solution of hormone (we tested beta-estradiol, DHEA, testosterone and others), we used to dissolve in EtOH at first, because hormone dissolves better, and then we added the medium. And then we did dilutions to have an EtOH final concentration of 0,1-0,2%. I don't remember it very well, but if you need it I can check... anyway it depends on the cell line, so you sholud firstly do a "growing curve" for your cell line in EtOH at different concentration to see what's the maximum concentration of EtOH your cells can tolerate.

Thank you very much for your replies.

with regards