Cell treating - Do you... (Nov/13/2008 )
When treating cells with a drug or any other biomolecules,
Do you:
1. mixed the drug to the right concentration in new medium, then replace the old medium?
2. adjust to a higher concentration before adding into the medium to get to the right concentration? no medium replacement here
i used number 1
Cheers
Do you:
1. mixed the drug to the right concentration in new medium, then replace the old medium?
2. adjust to a higher concentration before adding into the medium to get to the right concentration? no medium replacement here
i used number 1
Cheers
I do it by the second method - but I have co-treatments (ie I add one drug in fresh media, then I add the second drug at a higher working concentration to get the correct concentration I want!)
Do you:
1. mixed the drug to the right concentration in new medium, then replace the old medium?
2. adjust to a higher concentration before adding into the medium to get to the right concentration? no medium replacement here
i used number 1
Cheers
I do number 1. With number 2, if the drug/molecule has degraded, you have no way to know.
It depends..For me? I have been trying both of them. For example, I do make the right concentraion in media and replace the old medium, usually when I use 96 wells or bigger well-plates. But incase of 6 well or dishes, I do directly add (stock conc) to the cells that already flooded with fresh medium.
I do care about homogenous drug environment, final concentration of vehicle such as DMSO, alteration of pH (if any), drug stability and solubility. Also, ideal, easy, ethical and economical way we should consider. What you think??
I also do number 1.