Does sonication of cell lysate.... - (Feb/08/2008 )
allow me to skip the centrifugation step to remove debris ?
Thanks !
-deprapantere-
Don't you sonicate before you spin? I think I might be misunderstanding the question.. after sonication, you need to centrifuge because there are cell bits everywhere.
-Judes-
you have to centrifuge after sonication or even after boiling to get rid of debris.
-scolix-