Phoenix 293T packaging cells - (May/26/2007 )
I have some hand me down Phoenix cells for retroviral packaging but they have not been selected for years. I have had problems with viral production from these cells.
Any postdocs in the New England area or anywhere have a good stock of Phoenix cells.
I have various other cells lines I could swap, e.g. EcoPack293, PT67 and so on.
Phoenix cells are not an absolute requirement for production of retroviruses. You can easily obtain a good result by using 293T cells.
Quite right. try to get some 293T's, it works equally good.
why don't you just re-select them? that usually helps significantly.