Can adherent culture be transformed into suspension cells? - (Oct/16/2006 )

I need to transform adherent culture into suspension cells. Does anyone has any idea of how to approach it? I tried to take away FBS in the media and cells don't grow well. Please advice.

-sRunner-

QUOTE (sRunner @ Oct 17 2006, 06:37 AM)

I need to transform adherent culture into suspension cells. Does anyone has any idea of how to approach it? I tried to take away FBS in the media and cells don't grow well. Please advice.

It seems to me that it's not a good idea. Couldn't you use another cell line in which cells are non-adherent?
To me, to induce a such change in the phenotype will change many things in your cells.

-behappy736-

technically spoken it should work after trypsination and transfering your cells to suspension culture under shaking;

HOWEVER, are your cells able to grow and proliferate in suspension? for instance, epithelial cells become anoikic if detached from matric/ECM; so, follow the documentations and experiences to your type of cells or offer your type of cells to the community so that it can be discussed in more detail

-The Bearer-

What cell line are you using? What's your goal with moving to suspension?

-tetrakaidecagon-

I once cultured L cells and plated them in a non-coated petri dish (that is a mistake), however, these L cells transformed to suspension cells and seems to grow well.

-yeping-