Can adherent culture be transformed into suspension cells? - (Oct/16/2006 )
I need to transform adherent culture into suspension cells. Does anyone has any idea of how to approach it? I tried to take away FBS in the media and cells don't grow well. Please advice.
It seems to me that it's not a good idea. Couldn't you use another cell line in which cells are non-adherent?
To me, to induce a such change in the phenotype will change many things in your cells.
technically spoken it should work after trypsination and transfering your cells to suspension culture under shaking;
HOWEVER, are your cells able to grow and proliferate in suspension? for instance, epithelial cells become anoikic if detached from matric/ECM; so, follow the documentations and experiences to your type of cells or offer your type of cells to the community so that it can be discussed in more detail
What cell line are you using? What's your goal with moving to suspension?
I once cultured L cells and plated them in a non-coated petri dish (that is a mistake), however, these L cells transformed to suspension cells and seems to grow well.