yeast specialists - strange behavior in yeast cells (Apr/05/2008 )
Are there any people here that are very experienced in working, studying yeast?
And then I really mean very experienced, because I am looking for some answers to a questions that seems almost impossible to find an answer too.
I allready spoke to some specialist (senior researchers, prof...) about it and they couldnt even give me an answer.
If so let me know and I'll ask you the question and or send you a movie about my problem.
Hi Pito,
why don't you try to explain your problem shortly? Maybe there is someone who is not a great expert but had do deal with the same problem and was able to solve it.
why don't you try to explain your problem shortly? Maybe there is someone who is not a great expert but had do deal with the same problem and was able to solve it.
I can indeed do that.
I couldnt do it previsiously because its wasnt sure if I was allowed to show this.
The problem is that I see movement in yeastcells itself.
I have a movie that shows yeastcells in wich you will see little black dots jumping around in the yeastcells themself.
So in short: you see big yeastcells and in those cells you will see tiny black dots jumping around in some sort of vacuole.
The question now is: what are those dots and why do they move.
The movement is , I think, caused by brownian movement.
the following link will give you the option to download a movie in wich you can see this strange behaviour.
www.mediafire.com/?xnimjfco1my
Any ideas about this?
It seems to me, that you observe this black point always in cells which have a very big vacuole. I observed similar things (although not that black, but this may depend on the microscope mode) in my cells, but this cells seem to be dead. Maybe that are membrane inclusions in the vacuole which occur when your cells are really stressed. Normally this cells had a fragmented nucleus which I checked by DAPI staining. I saw this moving dots also in fixated samples so I thought it is an preparation artefact, some inclusions in the vacuole or bacterial contaminations. But anyway, for my analysis I never used this cells, because they looked more dead than alive.
Greetings
Greetings
its not a contamination and I think the cells are not all dead.
I cant however tell if the cells show this behaviiour are dead or not.
Greetings
its not a contamination and I think the cells are not all dead.
I cant however tell if the cells show this behaviiour are dead or not.
It seems that its observed more in several labs , but no one can tell what it is exactly is.
However research on this topic is going on in a certain lab, but they do not seem to eager to help lol
couldn't open the file butyour description seems consistent with brownian motion.
@jorge1907,
the link does indeed not work.
I have uploaded it again at: http://www.mediafire.com/?xnlnjtwstma
Its a movie file.
It is possible you need to download a certain codec to play it.
thanks pito - still couldn't open and got a message, as you suggested, that codec was needed (I'm too stupid to know what that means).
LOl jorge1907, I have seen that behaviour before... When I tried to open it
after a few hours of searching I found this:
http://www.divx.com/divx/windows/download/...CFQ9WtAodMXPEZA
I have downloaded it and since then I am able to play almost everything.
Maybe it works for you too?