trypsin/EDTA passaging problem - (Feb/15/2006 )
Hello,
I ran into a little problem with passaging cells. I am working with a rapid growing breast cancer cell line. Normally, I split them with trypsin about every 2 weeks. However, I split them twice yesterday one right after another. How would this affect the cells? I never did this before and it was an accident. I did see some cells growing today in my flask, but they were very sparse. How will my cells be affected? Thanks.
-nk111-
QUOTE (nk111 @ Feb 15 2006, 01:25 PM)
Hello,
I ran into a little problem with passaging cells. I am working with a rapid growing breast cancer cell line. Normally, I split them with trypsin about every 2 weeks. However, I split them twice yesterday one right after another. How would this affect the cells? I never did this before and it was an accident. I did see some cells growing today in my flask, but they were very sparse. How will my cells be affected? Thanks.
I ran into a little problem with passaging cells. I am working with a rapid growing breast cancer cell line. Normally, I split them with trypsin about every 2 weeks. However, I split them twice yesterday one right after another. How would this affect the cells? I never did this before and it was an accident. I did see some cells growing today in my flask, but they were very sparse. How will my cells be affected? Thanks.
The simplest guess is that since they weren't adhered and you added trypsin for the amount of time you did the 2nd time, you had some loss. The ones that are growing are likely just fine.
-immuno9-
Hi,
Thanks for the reply. Do you think they will grow to confluency (or even semi confluent) by early next week (ie Monday)? That is when I will need the cells for an experiment I'm doing, but it has stalled because of this cell loss.
-nk111-
cancer cells support good the splitting (as they are from cancer...)
QUOTE (nk111 @ Feb 16 2006, 01:26 AM)
Hi,
Thanks for the reply. Do you think they will grow to confluency (or even semi confluent) by early next week (ie Monday)? That is when I will need the cells for an experiment I'm doing, but it has stalled because of this cell loss.
Thanks for the reply. Do you think they will grow to confluency (or even semi confluent) by early next week (ie Monday)? That is when I will need the cells for an experiment I'm doing, but it has stalled because of this cell loss.
depends of the growing rate of your cells in your standard conditions...
-fred_33-