Hi All,
I hope that anyone of you could indicate me whether and how is possible to label a single protein (such as a proteasomic subunit) in "living" cells and track the label by fluor. microscopy.
I would like to perform a time-laps experiment and follow the effect of the treatment on the re-comportamentalization of that target.

Thanks a lot in advance.

Davide
Temple Univ.
Philadelphia

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Hey Davide, you can definitely do that, provided you have a good fluorescent microscope and a good antibody to your protein, or some other way to tag your protein. There are actually a few ways to do it. I'm not an expert in this, but if you give me some more info I could probably help you in the right direction.

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@Jon Peterson

Thanks a lot for your help.
I would to follow the migration of one of the subunit of Proteasome in a primary neuronal culture under a drug-treatment.
I need to keep a single cell under analysis during a time-lapse with a quite fluorescent microscope.
I am wondering if there is some methods that allows to check that tracking without interferring (or minimiz. the interference) of the antibody or -tag or whatever I have to use for this purpose.

I will appreciate any helpful suggestion you would give me.

Davide

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Heres a link to a paper that might help you out, I only read the abstract but should help you with the easy part of your problem, which is the mater of having the right kind of scope. The harder part is going to be getting a trackable protein into your system.
http://www.ncbi.nlm.nih.gov/entrez/query.f...4&dopt=Abstract

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