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freezing cells in log phase - (Jul/04/2007 )

Hi,

I am new to tissue cell culture work and I was wondering why is it important to freeze cells in log phase. All the protocols state that cells should be frozen 24hrs after passaging the cells. What happens if we freeze the cells immediately after passaging. By mistake I have frozen cells immediately after passaging ....now I am not sure if I can use these frozen cells again or no?

Any help will be appreciated.

Thanks for your input.

-baboon-

I would just thaw a vial and see if they grow!!

-lauralee-

QUOTE (baboon @ Jul 5 2007, 02:27 AM)
Hi,

I am new to tissue cell culture work and I was wondering why is it important to freeze cells in log phase. All the protocols state that cells should be frozen 24hrs after passaging the cells. What happens if we freeze the cells immediately after passaging. By mistake I have frozen cells immediately after passaging ....now I am not sure if I can use these frozen cells again or no?

Any help will be appreciated.

Thanks for your input.


the log growing phase is characterized by intense cell division, a feature that is highly useful after thawing and seeding out of freezed cells;

-The Bearer-