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cell signaling & choice of the cell - (May/11/2007 )

To write the report about cell signaling, I have read many papers.
when they carry out the project of cell signaling, they choice NIH-3T3, HepG, HEK293 CELL.

WHY THEY USE THESE CELLS??

I want you to explain this reason~~^^*

thank you. rolleyes.gif

-shi21ne-

Those are cell lines that are used a lot, so that makes it easier to compare with others (and if you see a certain effect, it makes it easier to find out if others have seen similar stuff with these cells). They are easy to transfect/transduce (I know this to be true for 293, haven't used the others), and they are easy to culture.
(those are some reasons I can think of, there might be others too, but if I were to do research on cell signaling and wouldn't need a specific differentiated cell line, I would pick a cell line according to these criteria).

-vairus-

QUOTE (shi21ne @ May 12 2007, 02:09 AM)
To write the report about cell signaling, I have read many papers.
when they carry out the project of cell signaling, they choice NIH-3T3, HepG, HEK293 CELL.

WHY THEY USE THESE CELLS??

I want you to explain this reason~~^^*

thank you. rolleyes.gif


some faculties have their "pets": hepatologists like HepG2 a.o., gastroenterologists Caco2, AGS a.o., Nephrologists MDCK, HEK293 a.o., dermatologists A431 a.o.

some cell lines express pathways or proteins that others do not or rarely; so the choice of a cell line strongly depends on your molecular question

-The Bearer-

We worked with Calu-3 cells as a model for pulmonary epithelium. We also used acutely dissociated cells to check the Calu-3 work, though. Also, we checked the signalling pathways of the cells with a barage of agonists: methacholine, adrenaline etc.

-paraboxa-