Huvec cell number ? - (Jul/21/2005 )
I need some help on starting a Western blot.
Can anybody tell me what is the number of cells required to be able to detect your protein of interest when doing a Western blot or Immunoprecipitation ?
I work with cell cultures, specifically Huvec (human umbilical vein endothelial cells).
I am trying to detect for a protein by doing a Western, but don't know what number of cells to grow up to do it.
What would be enough cells ?
ex) grown in 24 or 12 or 6-well plate ?
or in a T-25 or T-75 or T150 flask ?
And what would be a good number of cells seeded in a specific plate ?
Huvec cells don't seem to proliferate very well and takes time to grow to sufficient number.
I would very much appreciate any input.
i use a 6well plate at 80% confluency to do a western.
but the proteins i study are good expressed in the cells.
For IP i use a 10cm plate.