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Why use PBS before trypsinizing cells - (Jan/26/2006 )


I have a question about PBS, what is the reason to use PBS before using trypsin? Is this for washing the old media from the cells?

-Roy van Heesbeen-

Wash away medium because medium contains serum which inhibitis trypsin.


Thank you for the fast replay!

-Roy van Heesbeen-

we use serum-free medium; we still wash with PBS prior to trypsinization to make sure cell wastes and spent media are removed


i did heard that medium contains too much salts for trypsin activity. (trypsin is generally in a EDTA solution)