endothelial cells transfection - (Mar/06/2006 )
I'm trying to transfect porcine aortic endothelial cells. I've tried several methods including Lipofectin, Effectene, AMAXA Programs. All of them make my cells elongated, which is a big problem, because I need them for shear experiments, so I can't use them because they already changed shape.
Could anyone give me some input in this?
Our lab routinely uses gencarrier-1 for HUVEC and primary bone marrow endothelial cell transfection with great success. We did not notice any obvious cell loss or morphological change during and after transfection.
tahnk you for answer. do you mind if i ask you to give more details. I've sruggling with this problem for so long!!!