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mycoplasma contamination and MEFs - (Sep/04/2006 )

Hi everyone,
does someone know what mycoplasma does on MEFs cell line (mouse embrionic fibroblast)? (alter proliferation rate, dna sintesys or alter espression of some protein)...I can't find anything online...

thx

Mario

-mario77-

I don't know specifically what it does to MEFs ... but ...

Mycoplasmas Can Cause:

"Alterations in cell growth rate
Inhibited or stimulated cellular transformation
Morphological changes, potentially deleterious
Altered DNA, RNA, and protein synthesis
Altered enzyme actions
Chromosomal abnormalities
Reduced or increased virus yields
Depeleted nutrients from growth media
Altered cell surface antigenic characteristics
Decreased malignancy of tumor cells"

"Mycoplasma Contamination: A Critical Concern in Tissue Culture Applications" by Christina Liu

It's definately not a good thing for your cells.

-jamie419-

thx...I know that it's not a good thing, I was just wondering what does in the specific to MEFs cell...because I don't know if it's worthy keep on going with the experiment or it's better stop for a while and wait the treatment... ph34r.gif




QUOTE (jamie419 @ Sep 5 2006, 08:11 AM)
I don't know specifically what it does to MEFs ... but ...

Mycoplasmas Can Cause:

"Alterations in cell growth rate
Inhibited or stimulated cellular transformation
Morphological changes, potentially deleterious
Altered DNA, RNA, and protein synthesis
Altered enzyme actions
Chromosomal abnormalities
Reduced or increased virus yields
Depeleted nutrients from growth media
Altered cell surface antigenic characteristics
Decreased malignancy of tumor cells"

"Mycoplasma Contamination: A Critical Concern in Tissue Culture Applications" by Christina Liu

It's definately not a good thing for your cells.

-mario77-