why my IFN gamma treated cells still high in TER? - (Mar/25/2006 )
According to my knowledge, IFN gamma would disrupt tight junction and cause disease. So I cultured T84 mololayer cells on transwells and treated with IFN gamma (100ng/ml) after cells are confluence at day 12. The criteria of confluence is the transepithelial resistance (TER) reached stable around 12-13.
But, 24 hours continous exposure to IFN didn't result in tight juction disruption. The TER remains as high as 10. I would expect the TER dropping to at leat 5 if the tight juction is no longer contact.
(I used Corning transwells 3470 which has a 0.33cm2 area and I seeded cells at a density of 1*10^6 cells/cm2; IFN gamma is from sigma and is quite new, aliquoted at 17/11/05)
Can anybody see anything problem with my experiments? Is there any problem in cells or medium or contamination may affect IFN gamma effect? I have no idea what's going on. Any comments please???
from my experience it is difficult to see changes in low teer values.
if you can try another cell line