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Imaging cells in matrigel - how to stain ? Prepare ? (Aug/08/2006 )


I am about to start 3d cultures of cells in Matrigel. I'd like to know how to image the cells after it, since I'd like to look at the 3d structures that these cells form. How do I prepare/stain the cells ?



May b u could take stacks in the z-plane and then reconstruct the whole image of the cell.

I am not sure what do u want to look at. I am afraid, if u prepare the cells, u might lose the 3D part of the cells.

Best would b to image them live and use dyes which can b used in living cells.