cos-7 cells transfection using calcium phosphate - (Nov/08/2007 )
I need to transfect cos-7 cells using calcium phosphate transfection,I tried it first yesterday w. standard protocol,however, the efficiency is really low
should I use glycerol shock or other treatment to enhance efficiency? much thanks!
Calcium phosphate transfection always needs optimization. Try it a couple of more times with different conditions. Also try HBS with different pH levels.
good luck !!