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Inturpreting cryopreservation data - I need help applying data I'm colleting with the freezing of E. coli cells t (Feb/18/2006 )

I need some help applying the data I found to the "real world", whether it be in just cryobiology or in science in general. The information can stretched and interrupted anyway.

I grew two cultures of E. coli in two levels of glycerol, 0.1% and 10%, as well as the culture medium.

I then monitored the growth rate of the two cultures.

Rather than adding a cryoprotectant before freezing, I just froze them.

There are 4 cultures and they are all being thawed at different times; one immediately, 72 hrs, 1 week, 2 weeks.

I have only thawed the ones up to 72hrs and so far the cultures that were grown with 10% glycerol do not have a lag when they are thawed but grew slower initially.

I'm not sure if I have provided enough information for you to help me out but my main question is can this information be used to determine anything. Thank you so much for taking the time to read this. If you need any more information or clarification on anything please ask.

Thanks again,

ph34r.gif 12345_

-12345_-

QUOTE (12345_ @ Feb 18 2006, 02:47 PM)
I need some help applying the data I found to the "real world", whether it be in just cryobiology or in science in general. The information can stretched and interrupted anyway.

I grew two cultures of E. coli in two levels of glycerol, 0.1% and 10%, as well as the culture medium.

I then monitored the growth rate of the two cultures.

Rather than adding a cryoprotectant before freezing, I just froze them.

There are 4 cultures and they are all being thawed at different times; one immediately, 72 hrs, 1 week, 2 weeks.

I have only thawed the ones up to 72hrs and so far the cultures that were grown with 10% glycerol do not have a lag when they are thawed but grew slower initially.

I'm not sure if I have provided enough information for you to help me out but my main question is can this information be used to determine anything. Thank you so much for taking the time to read this. If you need any more information or clarification on anything please ask.

Thanks again,

ph34r.gif 12345_


I am not sure if this is the answer you are looking, but in general,you can apply this data to E. coli . Also any culture will usually show little lag after thawing. For E.coli we use 10-25% glycerol For different celllines we use 5-10% DMSO.

-Soni-