red blood cells and the HA Assay - differences between species (May/21/2008 )
I'm currently using RBC's for haemagglutination. Previously I have worked with goose rbc's and am now using chicken and guinea pig. during the wash process (mix with PBS and centrifuge) the goose and chicken behave nicely giving a nice clean pellet and clear supernatant. The guinea pig not so much - i can see a distinct pellet but the supernatant is still red. i've tried dividing my quantities so the concentration is lowered but still have a red haze. the blood is just as fresh as the chicken so not sure why.
does anyone else have experience with these that could help?
thanks in advance
does anyone else have experience with these that could help?
thanks in advance
Is there a difference in blood withdrawl techniques?. If you are using wider bore neele or canulation for chicken and narrow bore for guinea pig, this might damage some RBCs. Same anticoaggulant and same volume of blood withdreawn?
Someone had to remind me this one that I did not want to read then. 
Synder G, Sheafor B. Red Blood Cells: Centerpiece in the Evolution of the Vertebrate Circulatory System . Animal Zoologists. 1999 ;39(2):189-198.
does anyone else have experience with these that could help?
thanks in advance
Is there a difference in blood withdrawl techniques?. If you are using wider bore neele or canulation for chicken and narrow bore for guinea pig, this might damage some RBCs. Same anticoaggulant and same volume of blood withdreawn?
both stored in alsevers by the supplier and receive 25ml of each although this comes from a pool - more animals are in the guinea pig pool than the chicken pool. not sure about the withdrawal techniques but have asked the supplier for details. even today - a day after washing the chicken has settled nicely but the guines pig still has a hazy region above the pellet which to me seems like lysis but cells look fine. in addition when using in HA Assay the GP RBC's don't pellet fully but produce a ring-like structure rather than a full pellet. again i'm not sure why this is - this happens even if i do the assay on the day blood is received
Nothing to do with this post, but just a related incidence.
I wanted to buy some rat serum, one company charged 5 times more than the other. So I called them up and asked why?
They said they bleed the animals in a humane way (using venous canulation), whereas the other company just cuts the heads of off animals, so they do it faster and cheaper and get more blood/serum.
Both sera worked well ( I ordered both) in my embryo culture.
Why I shared it? I dunno. 
That's inhumane
That's inhumane
