protein isolation from cell lines - membrane bound proteins (Dec/23/2007 )
hello every one,
i need to isolate membrane bound proteins from animal cells (using cancer cell line grown in lab condition)is any body have procedrue -how to isolate membrane bound proteins from cell lines.
thanks in advance,
What kind of membranes are you interested ? membrane-proteins in general or membranes of special organelles ? differential centrifugation should be the method of choice...
...in combination with sucrose gradients...
Hi, I'm interested to extract a transmembrane protein from a specific organelle in the cell line. What protocol can I use?