lost contact inhibition=oncogenic cells? - (Jan/18/2008 )
My NMuMG cells grow very differently after being introduced with a gene, they not only form a monolayer in culture but also pile up on top of one another in foci.
This epithielial cell line is adherent, i.e. they do carry the feature of contact inhibition,right?
so are my cells oncogenic cells now???
-Cathleen-
Not necessarily. Sometimes normal cells pile up. You have to do more testing such as soft agar assay to see the cells form colonies in soft agar and xenograft tumor formation in nude mice.
-pcrman-
QUOTE (Cathleen @ Jan 18 2008, 11:06 AM)
My NMuMG cells grow very differently after being introduced with a gene, they not only form a monolayer in culture but also pile up on top of one another in foci.
This epithielial cell line is adherent, i.e. they do carry the feature of contact inhibition,right?
so are my cells oncogenic cells now???
This epithielial cell line is adherent, i.e. they do carry the feature of contact inhibition,right?
so are my cells oncogenic cells now???
do you transformed with a oncogene? then you may have oncogenic transformed cells; you may analyze cadheris, catenin, focal adhesion site, claudins, occludins...; in addition to pcrman, you may analyze migration...
-The Bearer-
QUOTE (pcrman @ Jan 18 2008, 02:13 PM)
Not necessarily. Sometimes normal cells pile up. You have to do more testing such as soft agar assay to see the cells form colonies in soft agar and xenograft tumor formation in nude mice.
how is a soft agar assay performed? what is the aim of this method?
-The Bearer-