tumoral cells against fibroblasts - (May/13/2005 )
I would like to know if there is another method (except buying the expensive Panomics isolation kit) to isolate cancer cells.
In fact, after collagenase digestion, even if I plate the digestion, let it settle for 1 hour to make fibroblast adhere and then pipet the supernatant into a new flask, I always obtain a mixed population.
Then, if with different times of trypsinisation I manage to separate the populations (fibroblasts detach before tumoral cells), the remaining tumoral cells grow very slowly and the very few remained fibrblasts overgrow in the following days!
can anybody help me?
at present i am trying to grow bladder tumor cells from the primary tumor or xenografts from the nude or NOD/SCID mice. the medium i used is RMPI based serum free medium, it works well for me. there is only a few or even no fibroblast in my flask. if you are interested in this method, i would tell you in detail.
yes I would like to know your protocol in detail if possible... I also use RPMI+ 10% FBS. Thank you again