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Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (Jan/18/2006 )

Hi there!!

I have a question regarding the culturing of Raji cells (B-cell lymphoma). Following one scientists recommendation, I'm currently culturing them in DMEM (high glucose, without pyruvate, with glutamine) supplemented with 10% FCS. However, all the paperwork and cell banking places suggest RPMI (+10% FCS) as the optimal growth medium. I've been growing them in DMEM and they're growing and viable, but they're just not fast growing - I've been told they grow like weeds, but at the moment they're lagging a bit...

Does anyone have any experience in culturing Raji cells, and what medium might be the most suitable?!

Thanks, guys!

Gayle

-GayleRitchie-

Hi,

we actually culture the Raji cell line in RPMI 1640 supplemented with 10% FBS and genta, nothing else, in 5% CO2 incubator in humidified atmosphere; the cells grow very well in these conditions

S├ębastien_

-tryptofan-

Thanks, Sebastien! That's really helpful! We may have to convert to the RPMI, then, if they don't perk up!!

Cheers,

Gx

-GayleRitchie-

Oooooh, Sebastien, just one more thought: does your RPMI medium contain any glutamine?? You didn't comment on it in your reply; I've been suggested to supplement Raji cell medium with stable forms of glutamine (the dipeptide L-ala-L-Gln), even if the medium comtains Gln already. Is this absolutely necessary, or can I proceed without this supplement?!

Thanks,

Gayle

-GayleRitchie-

oh yes, you are right!
our RPMI medium effectively contains ultraglutamine (the dipeptide L-Ala-L-Gln), so we don't add glutamine any more...
but i don't know if it's realy necessary, i haven't test RPMI w/o glutamine nor ultraglutamine...
i'm sorry wink.gif

S├ębastien_

-tryptofan-