How to grow adherent cells without attachment??? - (Aug/22/2005 )
Hiya!
Need help urgent...
I am looking for a way to grow U2OS cells (adherent) in a manner where they do not attach to the substrate base.
What flask materials or treatment can I use?
I have tried using the normal petri dishes for growing bacteria but the cells attached to grow! Short of using Falcon tubes, I am running out of ideas already!
Appreciate your inputs...
Cheers!
-MadScientist74-
QUOTE (MadScientist74 @ Aug 22 2005, 01:47 AM)
Hiya!
Need help urgent...
I am looking for a way to grow U2OS cells (adherent) in a manner where they do not attach to the substrate base.
What flask materials or treatment can I use?
I have tried using the normal petri dishes for growing bacteria but the cells attached to grow! Short of using Falcon tubes, I am running out of ideas already!
Appreciate your inputs...
Cheers!
Need help urgent...
I am looking for a way to grow U2OS cells (adherent) in a manner where they do not attach to the substrate base.
What flask materials or treatment can I use?
I have tried using the normal petri dishes for growing bacteria but the cells attached to grow! Short of using Falcon tubes, I am running out of ideas already!
Appreciate your inputs...
Cheers!
Have you tried glass? Cells don't stick to glass.
babynekko
-babynekko-
Many cells require attachment and cytoskelatel spreading in order to replicate. The cells may not grow without attachment and will almost certainly have different charcteristics to attched cells. Cells require a mechanical stress on the cytoskeleton to gorw and divide (Ingber 2003 J Cell Sci 116(8) 1397-1408 is an interesting article about this).
-ajames-