How to separate the antigen presenting cell from spleen of rat - (Apr/18/2007 )

Hi
Can anybody help me to know-how I can separate the antigen presenting cell from spleen of rat.
Thanks
Tofael

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If by antigen presenting cells you mean only macs and dcs and not B cells then you can do a simple adherence step using plates. This allows antigen presenting cells to adhere to the plate and B cells, NK cells, and T cells to remain in the media. There are also several kits you can use to isolate macs and dcs through negative selection that are rat specific.

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