I need help for my Raji cell culture - (Oct/23/2008 )

Hi, everybody. I'm a researcher from Thailand and I have to study in Japan for doctoral degree. But I have a problem because japanese students don't talk in english much. So, my research have to deal with the Raji-cell culture. I'm not sure the Raji cell is B-lymphoma, It's right or wrong.Thus, I would like to recieve the information such as , the protocol for culture Raji-cell, the suitable medium, the suitable time for do experiment with the raji-cell after we take them from the freezer. I have ever cultured a HL-60 cell for apoptosis activity assay. But in the Raji-cell I have to do the research about NF-kB assays. So I need some help about the basic to culture this cell. Thank you very much for your help and your time.

Hi,

Rajis are human B lymphocytes (isolated from 37-year-old male with Burkitt's lymphoma). I culture my Rajis in DMEM + GlutaMAX™ (Gibco, 31966) + !0% heat-inactivated foetal calf serum and 1% penicillin/streptomycin.

I find that Rajis grow really fast once they are up and running and so I usually do a 1:10 subculture every 2-3 days. I am not sure about the differences between passage number of these cells but I have used my cells at a fairly high passage and they seem fine.

The only other thing is that if you are using LPS as an activator, you may want to consider using endotoxin-low FCS as endotoxin can prime the cells and reduce their response to stimulation.