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Arabidopsis cells suspension transformation - (Jan/18/2006 )

Hello everybody,
I want to transform an Arabidopsis cells suspensions via agrobacterium.
Does anybody have a nice protocol??

-Fredo-

Nobody have a protocol to do that??

-Fredo-

Hi,
I tried once a published method. It didn't work with the cells I was using, but the method itself seems fine to me.

Forreiter et. al, 1997. Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo. The Plant Cell, 9, 2171-2181.

Bye,
Andrea

-andrea massimo-

QUOTE (Fredo @ Jan 18 2006, 11:08 AM)
Hello everybody,
I want to transform an Arabidopsis cells suspensions via agrobacterium.
Does anybody have a nice protocol??



there's an article from Menges et al., published about transformation of Arbaidopsis cell suspension, I think plant journal

I don't know if they uses Agrobacterium tough
grtz

-Thomas.devijlder-