Arabidopsis cells suspension transformation - (Jan/18/2006 )
Hello everybody,
I want to transform an Arabidopsis cells suspensions via agrobacterium.
Does anybody have a nice protocol??
-Fredo-
Nobody have a protocol to do that??
-Fredo-
Hi,
I tried once a published method. It didn't work with the cells I was using, but the method itself seems fine to me.
Forreiter et. al, 1997. Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo. The Plant Cell, 9, 2171-2181.
Bye,
Andrea
-andrea massimo-
QUOTE (Fredo @ Jan 18 2006, 11:08 AM)
Hello everybody,
I want to transform an Arabidopsis cells suspensions via agrobacterium.
Does anybody have a nice protocol??
I want to transform an Arabidopsis cells suspensions via agrobacterium.
Does anybody have a nice protocol??
there's an article from Menges et al., published about transformation of Arbaidopsis cell suspension, I think plant journal
I don't know if they uses Agrobacterium tough
grtz
-Thomas.devijlder-