Cell clumping in culture: Folic acid or i-Inositol? - (May/15/2008 )
I am using a medium that I prepared by myself according to the description sheet of the producing company (being cheaper that way), but I am getting abnormal cell clumping on the very next day. The original medium is Myelocult, and it contains MEM, Folic Acid, i-Inositol, L-glutamine, FBS, Horse serum, 2-Mercaptoethanol. When I cultured same cells from same sample with RPMI medium, and the prepared medium, clumping occured in the prepared medium only. I am guessing it is either the Folic acid or the i-Inositol, being the 2 constituents supplied in powder, and the weighing was difficult.
Do you know which of these 2 would cause cell clumping if increased or decreased in the culture medium??
No Idea. I'd prepare two more sets of medium. Each without one the suspect constituents.
Actually, I'd probably just buy the media from the distributor .
True. Especially, if you are working in Japan
Would have done it gladly, but my professor says it is too expensive, we can prepare ourselves