cell cultur medium - (Jul/30/2008 )

dear all,
i am in ruptur of minimum essential medium, now is it possible to prepar my complete medium without D-MEM?.With what can i replace the MEM which play the same role?
for 50ml of complet medium i use : 43,5 ml of RPMI + 5 ml of FBS + 0,5 ml MEM + 0,5 ml of L-GLU + 0,5 mL of STREP-PENICYLINE.
I prepar the complete medium to thaw PBMCs frozen in nitrogen liquid and to do three hours of cell cultur whit PMA + IONOMICIN + BREFELDIN A.
thanks for helps

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you try to improve your medium to kill your cells with the cocktail of toxins you describe?

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i just want to know if it is possible to prepar a complete medium without MINIMUM ESSENTIAL MEDIUM.

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Wow steady on partner.

MEM contains all the many Vitamins, Salts, Glucose etc required for long term maintenance and culture of cells. There are many different variations on the "Theme":
Waymouth's
Iscove's
Hams
MCDB 131
and RPMI etc etc etc


For short term work (3hours) you may get away with PBS + 10% FCS + 1000mg/L L-Glucose. Hope this calms you down.

Required reading: Nutrition Needs of Mammalian Cells in Tissue Culture.
Science, September 16, 1955, 122 (3168): 501–504.
By Harry Eagle....the Godfather of cell and tissue culture.

Kindest regards

Rhombus

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