Measuring cells in micrographs - (Mar/23/2008 )
Dear all,
I have micrographs (from LM and TEM) and I need to measure the size of bacteria cells. Do I do this based on the bars given? Say, i have a bar that measures 1 cm as 10 um. So, if my cell is 0.5 cm, does that correspond to a width of 5 um?
Along the way, I got confused on how to use the bars to calculate magnification. I have a 2.5cm bar which measures 200 nm. Is it correct if I change 2.5 cm to nm and then dividing it by 200?
Thanks people.
-dreamchaser_jc-
QUOTE (dreamchaser_jc @ Mar 23 2008, 12:18 PM)
Dear all,
I have micrographs (from LM and TEM) and I need to measure the size of bacteria cells. Do I do this based on the bars given? Say, i have a bar that measures 1 cm as 10 um. So, if my cell is 0.5 cm, does that correspond to a width of 5 um?
Along the way, I got confused on how to use the bars to calculate magnification. I have a 2.5cm bar which measures 200 nm. Is it correct if I change 2.5 cm to nm and then dividing it by 200?
Thanks people.
I have micrographs (from LM and TEM) and I need to measure the size of bacteria cells. Do I do this based on the bars given? Say, i have a bar that measures 1 cm as 10 um. So, if my cell is 0.5 cm, does that correspond to a width of 5 um?
Along the way, I got confused on how to use the bars to calculate magnification. I have a 2.5cm bar which measures 200 nm. Is it correct if I change 2.5 cm to nm and then dividing it by 200?
Thanks people.
yes and yes. the bar is the scaling standard.
-mdfenko-