Cell Banking - cell splittting and banking (Oct/28/2006 )

Hi all,

Can we split the cells today and bank them the next day? Suppose, I had 8 flasks and thought that I might not get enough cells if I harvest them for banking, so can I do 1:2 split today and bank them the nest day? I am taking about adherent cells. Will this be ok or do you anticipate any problems?

thanks for your replies.

Scifi

Hi!

as far as I know, splitting cells too often means stressing them too much
cells could have problem in facing the banking process, they could die during freezing so when you unfreeze (correct word? ) you have less cells than you expected.
but it also depend on how "delicate" is your cell line! Hope your is the strongest one!