Is my RNA dead? - freezing cells after adding RNA lysis buffer (Dec/01/2006 )
Hi!
I am new to this forum, but hoping it is the right one to pose my question in, which I haven't found the answer to anywhere:
I added RNA lysis buffer to my cultured cells and froze the mixture (-70). According to the Ambion protocol, one should not do this, but I am wondering why, and, is my RNA ruined now? Anybody know anything abt this?
/mia
-Miamia-
DO not worry. RNA lysis buffer lyses the cells and not RNA. So your RNA is safe at -80. You can follow the further protocol and get good RNA. All the best. Try searching for "RNA lysis buffer" and see what the search results say..
-Calvin*-
Great, thanks!
I can run RT-PCR today then, without fear.
-Miamia-