FACSing of MCF-7 cells - (Sep/19/2006 )
I would like to FACS MCF-7 cells, but they come in clusters when I get them off the plate. I tried everything, triturating, digesting, the works. Any other idea how to get a single cell suspension with this cell line? Should I use a cell strainer to get rid of the big chunks?
check this thread.
I would suggest not to under or over trypsinize the cells.
try 26G syringe to break clumps