Is there any destructive effect on nucleic acid when harvest the cell by scrapin - (Nov/13/2008 )
I have to extract cell RNA from a T25 flask, and i'm going to first harvest the cell with cell scraper as part of the cells are required for protein extraction with RIPA lysis buffer . Will it cause any damage to the cell's RNA by scraping?
Thanks in advance for any replys.
There shouldn't be much direct damage to the actual RNA, though you will burst/damage a large number of cells which may cause an up-regulation of some genes unless you are quick with your harvest.