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Subculturing primary human fibroblasts - Seeding density? (Sep/12/2007 )

Hi there,

What is the common seeding density used for human primary fibroblasts?

Are the following numbers correct?

4.2 *10^4 cells / ml
1.25 * 10^5 cells / 25 cm2
3.75 * 10^5 cells / 75 cm2

Thanks!

-theOwl-

Anyone? How about other fibroblast cellines?

-theOwl-