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Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (Dec/10/2006 )

Hi guys!

I use competent Top10 E.coli cells in my Topo Ta cloning kit. I wonder if i can incubate these competent cells over night at 35 celcius degrees instead for 37celcius degrees?
Will the cells grow well?

Thanks for your help!

-seasons-

Yes, they will grow fine.

-phage434-

QUOTE (phage434 @ Dec 10 2006, 09:22 AM)
Yes, they will grow fine.


But i guess they will not grow optimally as at 37C ?

-seasons-

They will grow a little slower, probably not noticeably. Don't worry, be happy.

-phage434-

QUOTE (phage434 @ Dec 10 2006, 10:52 AM)
They will grow a little slower, probably not noticeably. Don't worry, be happy.


Thanks alot! laugh.gif

-seasons-