Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (Dec/10/2006 )
I use competent Top10 E.coli cells in my Topo Ta cloning kit. I wonder if i can incubate these competent cells over night at 35 celcius degrees instead for 37celcius degrees?
Will the cells grow well?
Thanks for your help!
Yes, they will grow fine.
But i guess they will not grow optimally as at 37C ?
They will grow a little slower, probably not noticeably. Don't worry, be happy.