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size of an endothelial cell - (Mar/12/2007 )

Hello all,

I want to isolate endothelial cells from the heart and the lung of mice.

I was curious to know what is the size of an endothelial cell??
Also, I have done the isolation procedure before, but I see my endothelial cell cultures contaminated with fibroblasts and smooth muscle cells.

The procedure for isolating the cells for using a cell strainer, having 70microm pore size.

If someone could give me suggetions, i would appreciate that.

thanks

Pooja

-p.maj-

QUOTE (p.maj @ Mar 12 2007, 09:26 AM)
Hello all,

I want to isolate endothelial cells from the heart and the lung of mice.

I was curious to know what is the size of an endothelial cell??
Also, I have done the isolation procedure before, but I see my endothelial cell cultures contaminated with fibroblasts and smooth muscle cells.

The procedure for isolating the cells for using a cell strainer, having 70microm pore size.

If someone could give me suggetions, i would appreciate that.

thanks

Pooja

the size of an lung mouse endothelial cell near 8um. May be you try to isolate your cells on immunoimmobilized magnetic beads. But you should carefully chose antibody to avoid cross reactivity with SMC.

-circlepoint-

Hi,

I use immunomagnetic beads and I coat them with anti-PECAM-1. is this anti-body the right one to use?

Thanks,

Pooja










QUOTE (circlepoint @ Mar 12 2007, 01:13 PM)
QUOTE (p.maj @ Mar 12 2007, 09:26 AM)
Hello all,

I want to isolate endothelial cells from the heart and the lung of mice.

I was curious to know what is the size of an endothelial cell??
Also, I have done the isolation procedure before, but I see my endothelial cell cultures contaminated with fibroblasts and smooth muscle cells.

The procedure for isolating the cells for using a cell strainer, having 70microm pore size.

If someone could give me suggetions, i would appreciate that.

thanks

Pooja

the size of an lung mouse endothelial cell near 8um. May be you try to isolate your cells on immunoimmobilized magnetic beads. But you should carefully chose antibody to avoid cross reactivity with SMC.

-p.maj-