cell density - (May/29/2008 )

It's generally agreed that cells behavior differently at different densities. The same treatment may lead to different result on cells with different densities. It's usually suggested that not let cells reach confluence and split then around 80% confluence. However, when talking about 80% or 30% or 100% confluence, I really don't have a strong impression what they look like. What an appearance is 80% confluence? or 100%?...This is a very subject and empirical thing and obviously, different cell types will have different criteria. Therefore, I am thinking if we could put some pictures of different types of cells at different confluences here, so that people can look at those pictures and get a direct and concrete impression about 80% or 100% of a specific cell type?

Especially, I'm working with MEFs. Could anyone put a picture of MEFs at 50%, 80% and/or 100% confluence?

Thanks.

-wantz-

QUOTE (wantz @ May 29 2008, 07:58 PM)
It's generally agreed that cells behavior differently at different densities. The same treatment may lead to different result on cells with different densities. It's usually suggested that not let cells reach confluence and split then around 80% confluence. However, when talking about 80% or 30% or 100% confluence, I really don't have a strong impression what they look like. What an appearance is 80% confluence? or 100%?...This is a very subject and empirical thing and obviously, different cell types will have different criteria. Therefore, I am thinking if we could put some pictures of different types of cells at different confluences here, so that people can look at those pictures and get a direct and concrete impression about 80% or 100% of a specific cell type?

Especially, I'm working with MEFs. Could anyone put a picture of MEFs at 50%, 80% and/or 100% confluence?

Thanks.

Yeah. A picture is ok！

-goldideas-

QUOTE (wantz @ May 29 2008, 07:58 PM)
It's generally agreed that cells behavior differently at different densities. The same treatment may lead to different result on cells with different densities. It's usually suggested that not let cells reach confluence and split then around 80% confluence. However, when talking about 80% or 30% or 100% confluence, I really don't have a strong impression what they look like. What an appearance is 80% confluence? or 100%?...This is a very subject and empirical thing and obviously, different cell types will have different criteria. Therefore, I am thinking if we could put some pictures of different types of cells at different confluences here, so that people can look at those pictures and get a direct and concrete impression about 80% or 100% of a specific cell type?

Especially, I'm working with MEFs. Could anyone put a picture of MEFs at 50%, 80% and/or 100% confluence?

Thanks.

reported confluency is often the result of an experienced but rough estimation

-The Bearer-