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Antibody with sodiem azide added to mammalian cells - (Jan/30/2006 )

Does anybody know up to what concentration of sodium azide would be toxic to the mammalian cells? The reason I am asking is I need to add antibody to the cultured cells and the antibody contains 0.02% of sodium azide. Should I make the antibody very very dilute the low the concentration of sodium azide? Thanks guys

-appleone-

Hi,

i know that traces of NaN3 are very effective, i have heard that 0.001% is still toxic for cells in culture...
i suggest you to pass you Ab on protein G (or L) column, to dialyse it or to pass it in concentrator (Centricon)

S├ębastien_

-tryptofan-

QUOTE (tryptofan @ Jan 30 2006, 02:01 PM)
Hi,

i know that traces of NaN3 are very effective, i have heard that 0.001% is still toxic for cells in culture...
i suggest you to pass you Ab on protein G (or L) column, to dialyse it or to pass it in concentrator (Centricon)

S├ębastien_


Hi Sebastien,
Thx for your suggestion--but how exactly to dialyze the Ab when passing on the protein G? Didn't quite get it.... Could you tell me some detail to do it? dry.gif Or will filter through the 0.2um filter help to get rid of the NaN3?
Thx a lot!!

-appleone-