What cell lines are good for lymphoma research? - Please recommend lymphoma cell line. (May/13/2007 )
I need a lymphoma cell line, more specifically, diffuse large cell lymphoma. Is there any available human diffuse large cell lymphoma cell line? Anyway, please recommend one.
I need a human diffuse large cell lymphoma cell line (or human lymphoma cell line) that...
1. needs common media (RPMI+FBS)
2. is easy to transfect
3. grows fast/well
4. is not easily detached from the plastic bottom (Actually I dont know whether lymphoma cell lines are adherent or not)
5. is commonly used - used in a lot of published research papers on lymphoma study
Thanks in advance.
What kind of lymphoma are you interested in (B-cell, T-cell, NK-cell)? EBV researchers use various EBV associated B-cell lymphoma lines (Burkitt's lymphoma/BL which are EBV positive or negative). Jurkat, a T-cell leukaemia cell line, is commonly used by T-cell researchers and THP-1 is a monocytic leukaemia. These cell lines will grow in RPMI plus 10% FCS and Jurkat and BL cell lines are transfectable by electroporation. They grow fast but they're all suspension cells as far as I know.
Try searching for "lymphoma" in the ATCC or the European equivalent (ECAC?).
DLCL is a kind of B cell lymphoma. The best thing is to get this specific cell line, but I know it is not available in ATCC. Can you recommend some good B-cell lymphoma cell line? (Actually DLCL is also non-Hodgikin lymphoma)
Is "DLCL" diffuse large cell lymphoma or some kind of lympoblastoid cell line? Burkitt's lymphoma is also a non-Hodgkin's lymphoma but how related to your lymphoma of interest I'm not sure. Maybe try to get hold of some kind of clinical samples is probably the most relevant thing, but hard to get hold of and work routinely on depending on your circumstances.
If it is diffuse large cell lymphoma cell lines you're after for non-hodgkin's lymphoma research. Then there are 2 on the ATCC website and they're called Toledo and Pfeiffer. So you could purchase them from ATCC or write to ask the guy who deposited them originally.