single cell DNA isolation frommammalian cells - (Aug/03/2001 )
Does anyone have a reliable protocol for isolating DNA from a single mammalian cell?
What do you actually mean from a single mammalian cell?
Yes Dimitris...from one single cell.
What do you mean from a single mammalian cell? Just one cell?
I will suggest the protocol I know that can be used for single sperm cells. I believe it is easily adapted to somatic cells. PNAS 95:10791. The protocol involves embedding in agarose, scraping single cells off with a blade, lysing, neutralizing and then using the sample for PCR.