Antibodies for immunohistochemistry and cell ELISA - Are they the same? (Nov/22/2007 )
Hello experts
Is it safe to say that when an antibody is applicable for immunohistochemistry (as informed in datasheet), it is also applicable for cell ELISA?
ps. by cell ELISA i mean the cells are grown in polylysine-treated 96 well plates and then subjected to ELISA (fixed with formaldehyde, permeabilized with Triton, blocked, incubated with 1st and 2nd antibody etc).
-chrismartin-
in general, maybe.
if the sheet specifies ihc but not elisa then it may not have the avidity necessary for elisa.
-mdfenko-
Depend on the antibody. You have to test to make sure.
-Almasy-