Protocol Online logo
Top : Forum Archives: : Cell Biology

COS7 cells doesn't attach to the flask - (Sep/11/2005 )

I have a problem with COS-7 cells- after Defrosting Vail from a liquid nitrogen the cells doesn't attache to the flask, even after few days. Does anyone is familiar with this problem? What could be the reason?


This strongly suggests that you have a bad vial of cells. Probably frozen in the wrong way.