Separate mammal cell - (Aug/21/2001 )

How do I use proteinase K for separating mammal cell? please help me. Thanks in advance.

Hi, Le van BinhI can help you, but please, clarify me that you need using proteinase K for simply separation of a mammalian cells or for proteolysis the cell following DNA isolation.Why you using proteinase K?Please, don't hesitate to contact me.Best Regards,Dmitriy V. Volokhov, Ph.D.