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Thawing of competent bacterial cells - (Mar/20/2005 )

How can I thaw competent bacterial cells for transformation? Can i thaw the tube within my hand or would this damage my cells?

-justwonder-

hi
it's better to thaw competent bacterias on ice then you strip it on a selective lb agar plate. Then pick one clone, and do the preculture, followed by culture smile.gif

-fred_33-

thawing the competent cells with hands will definitely harm them. its always better to thaw them by keeping them in ice.....

-dasgupta_arupratan-