Cell Culture protocol by Current Protocol - free online version from invitrogen (Aug/19/2008 )
Please check link for the Cell Culture protocol by Current Protocol
Thanx a lot it's been very helpful, but do you have any experience with cancer cell culture? How to make a primary culture, since my previous experience is only with fibroblast, which I have to use disect blade into the petridish from the skin biopsy.
I wonder if there is a CD5 negative cell lines and how can I get it. Please advice
Specific questions are best asked in their own topics, not this one.